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991.
Ahn A Yang C Wax A Popescu G Fang-Yen C Badizadegan K Dasari RR Feld MS 《Applied optics》2005,44(7):1188-1190
Harmonic phase-dispersion microscopy (PDM) is a new imaging technique in which contrast is provided by differences in refractive index at two harmonically related wavelengths. We report a new configuration of the harmonic phase-dispersion microscope in a Mach-Zehnder geometry as an instrument for imaging biological samples. Several improvements on the earlier design are demonstrated, including a single-pass configuration and acousto-optic modulators for generating the heterodyne signals without mechanical arm scanning. We demonstrate quantitative phase-dispersion images of test structures and biological samples. 相似文献
992.
Array detector-based instruments are now fundamental to measurements of ozone and other atmospheric trace gases from space in the ultraviolet, visible, and infrared. The present generation of such instruments suffers, to a greater or lesser degree, from undersampling of the spectra, leading to difficulties in the analysis of atmospheric radiances. We provide extended analysis of the undersampling suffered by modern satellite spectrometers, which include the Global Ozone Monitoring Experiment, Scanning Imaging Absorption Spectrometer for Atmospheric Chartography, Ozone Monitoring Instrument, and Ozone Mapping and Profiler Suite. The analysis includes basic undersampling, the effects of binning into separate detector pixels, and the application of high-resolution Fraunhofer spectral data to correct for undersampling in many useful cases. 相似文献
993.
Dammika?P.?Manage Yao?Zheng Martin?J.?Somerville Christopher?J.?BackhouseEmail author 《Microfluidics and nanofluidics》2005,1(4):364-372
Microfluidics provides a promising tool for meeting the growing demand for high-throughput and low-cost mutation detection technology. With conventional instrumentation, this need is often addressed by the combination of the single-strand conformation polymorphism (SSCP) and heteroduplex analysis (HA) methods. This paper describes an effective microchip-based method to analyse the three most commonly tested gene mutations (C282Y, H63D, and S65C) associated with hereditary haemochromatosis by simultaneously performing microchip-based SSCP and HA, directly upon samples of polymerase chain reaction (PCR) product. We have increased the sensitivity of mutation detection considerably by adapting and combining SSCP with HA. We are able to perform the analysis within several minutes by avoiding off-chip sample preparation steps for SSCP and HA (apart from the PCR). The most important mutation in the screening of populations for this disease is the C282Y mutation and this mutation has not previously been detected with methods of HA/SSCP suitable for microchip implementation. This is, to the best of our knowledge, the first microchip-based test applying SSCP and HA for all three of the most common HFE mutations. 相似文献
994.
Christopher T 《IEEE transactions on ultrasonics, ferroelectrics, and frequency control》2005,52(9):1523-1533
A measure of focusing efficiency is introduced for high-intensity, focused ultrasound (HIFU). The measure consists of the fraction of the total acoustic power emitted that linearly propagates through a circle located at the focus. The medium is absorption-free water, and power is computed using pressure and the normal component of velocity. 3 MHz phased-array designs involving different element layouts and curvatures are placed in square apertures of length 2.2 cm. The acoustic fields of these devices then are propagated to on-axis foci. The resulting focal efficiencies then are calculated using a two wavelength (0.1 cm) radius circle. Among these array designs, an annular array with 27 wavelength-wide rings then is extended to be the basis of a twin phased-array device for prostate hyperthermia treatment. The two annular arrays are attached to door-like hinges to allow for joint two-dimensional focusing. The focusing efficiency of this device then is compared to rectangular element-array devices with the same 5.4 by 2.2 cm source extent. With the addition of absorption and finite-amplitude distortion, the heating rate and temperature rise produced by the twin annular device in prostate tissue is considered. As a final look at the potential of annular array-based designs, three larger 2 MHz devices are briefly considered for abdominal treatment. 相似文献
995.
Hughes MS Marsh JN Hall CS Savéry D Lanza GM Wickline SA 《IEEE transactions on ultrasonics, ferroelectrics, and frequency control》2005,52(9):1555-1564
We describe characterization of digital signals using analogs of thermodynamic quantities: the topological entropy, Shannon entropy, thermodynamic energy, partition function, specific heat at constant volume, and an idealized version of Shannon entropy in the limit of digitizing with infinite dynamic range and sampling rate. We show that analysis based on these quantities is capable of detecting differences between digital signals that are undetectable by conventional methods of characterization based on peak-to-peak amplitude or signal energy. We report the results of applying thermodynamic quantities to a problem from nondestructive materials evaluation: detection of foreign objects (FO) embedded near the surface of thin graphite/epoxy laminates using backscattered waveforms obtained by C-scanning the laminate. The characterization problem was to distinguish waveforms acquired from the region containing the FO from those acquired outside. In all cases the thermodynamic analogs exhibit significant increases (up to 20-fold) in contrast and for certain types of FO materials permit detection when energy or amplitude methods fail altogether. 相似文献
996.
Hampson KM Munro I Paterson C Dainty C 《Journal of the Optical Society of America. A, Optics, image science, and vision》2005,22(7):1241-1250
It is fairly well established that the higher-order aberrations of the eye fluctuate over relatively short time periods, but as yet there is no conclusive evidence regarding the origin of these fluctuations. We measured the aberrations and the pulse pressure wave simultaneously for five subjects. The aberrations were measured by using a Shack-Hartmann sensor sampling at 21.2 Hz. We decomposed the aberration data into Zernike coefficients up to and including fifth order and also calculated the rms wave-front error. From the pulse data the heart rate variability signal was also derived. Coherence function analysis showed that for all subjects there was a weak correlation between many of the aberrations and the pulse and the derived heart rate variability. The pulse and the heart rate variability can account for only 11% +/- 2% and 20% +/- 2%, respectively, of the aberration dynamics. 相似文献
997.
Davies C 《Philosophical transactions. Series A, Mathematical, physical, and engineering sciences》2005,363(1830):1109-1118
The use of numerical simulations to study the development of boundary-layer disturbances is illustrated for a number of different incompressible flow configurations. These include cases where the disturbances are generated by, or interact with, flow-control devices in the form of compliant panels, suction slots and microelectromechanical systems actuators. The velocity-vorticity system of governing equations used for the simulations is reviewed, along with the numerical discretization. 相似文献
998.
Toward rapid, "green", predictable microwave-assisted synthesis 总被引:6,自引:0,他引:6
Continuous and batch microwave reactors were constructed for efficient, "green" synthesis with low-boiling solvents at high temperature in closed vessels. Capabilities for rapid heating and cooling, concurrent heating and cooling, and differential heating facilitated novel chemical reactions and processes. Commercial microwave systems based on these developments are available. Times required for conventional reactions typically are decreased by 2-3 orders of magnitude. Green processes also have resulted through use of less or no catalyst, readily recyclable solvents, or media and yields that are often higher than normal. Complementary interactive software for calculating optimal conditions was developed. 相似文献
999.
Lake IR Foxall CD Lovett AA Fernandes A Dowding A White S Rose M 《Environmental science & technology》2005,39(23):9033-9038
This paper presents the results of a study examining whether the flooding of pasture by rivers gives rise to higher PCDD/F and PCB concentrations in cows' milk. Over 180 milk, soil, and grass samples, taken from 38 farms across 3 different river systems (River Dee, Trent, and Doe Lea/Rother/Don) in the United Kingdom, were analyzed for PCDD/Fs and PCBs. The concentrations were compared between flood-prone farms, where the animals had access to pasture that is often flooded, and control farms where the land does not flood. The results indicated that concentrations of PCDD/Fs and PCBs in cows' milk were higher in samples taken from farms prone to flooding, but only from the river systems flowing through industrial and urban areas. Raised levels of PCDD/F and PCBs were also found in soil and grass from farms prone to flooding providing strong corroborative evidence that the higher concentrations in cows' milk from such areas is likely to be due to the ingestion of contaminated grass and soil. Overall, the results provide strong evidence that flooding of pastureland can indeed result in elevated concentrations of PCDD/Fs and PCBs in milk from the farms so affected. 相似文献
1000.
To provide a new perspective on the fate of petroleum in the marine environment, we utilized variations in the natural abundance of radiocarbon (14C) to detect and quantify petroleum residues that have persisted in Wild Harbor sediments, West Falmouth, MA, for more than 30 years. The 5730-yr half-life of 14C makes this isotope ideal for the detection of fossil-fuel-derived contaminants (14C free) within different fractions of natural organic matter (modern 14C content) in environmental matrixes. Samples of both contaminated and uncontaminated sediments were sequentially treated, first by solvent extraction, followed by saponification, and then acid hydrolysis. Radiocarbon analysis of the sediment residues and select extracts was performed to probe for the presence of fossil fuel contaminants and/or their metabolites in different pools of sedimentary organic matter. Our results indicate that the majority of fossil carbon is solvent-extractable and has not been incorporated in the insoluble organic matter in sediment. Unextracted sediments contaminated with petroleum contain significantly less 14C than extracted sediments, and isotope mass balance calculations suggest that up to approximately 9% of the total organic carbon (TOC) in the petroleum contaminated sediment horizons is derived from solvent-extractable petroleum. These estimates are similar to values calculated when the total quantities of oil (measured by gas chromatography with flame ionization detector (GC-FID)) are compared to TOC content (determined by elemental analysis). These results pave the way for applications of this isotopic approach to more complex environmental systems where the fate of contaminants is less certain. 相似文献