Analysis of miRNA Expression Profiling of RIP2 Knockdown in Chicken HD11 Cells When Infected with Avian Pathogenic E. coli (APEC)

Colibacillosis is an acute and chronic avian disease caused by avian pathogenic E. coli (APEC). Previous studies have demonstrated that RIP2 plays a significant role in APEC infection. Moreover, increasing evidence indicates that microRNAs (miRNAs) are involved in host–pathogen interactions and the immune response. However, the role of miRNAs in the host against APEC infection remains unclear. Herein, we attempted to reveal new miRNAs potentially involved in the regulation of the immune and inflammatory response against APEC infection, with a particular focus on those possibly correlated with RIP2 expression, via miRNA-seq, RT-qPCR, Western blotting, dual-luciferase reporter assay, and CCK-8. The results showed that a total of 93 and 148 differentially expressed (DE) miRNAs were identified in the knockdown of RIP2 cells following APEC infection (shRIP2+APEC) vs. knockdown of RIP2 cells (shRIP2) and shRIP2 vs. wild-type cells (WT), respectively. Among those identified DE miRNAs, the biological function of gga-miR-455-5p was investigated. It was found that gga-miR-455-5p regulated by RIP2 was involved in the immune and inflammatory response against APEC infection via targeting of IRF2 to modulate the expression of type I interferons. Additionally, RIP2 could directly regulate the production of the type I interferons. Altogether, these findings highlighted the crucial role of miRNAs, especially gga-miR-455-5p, in host defense against APEC infection.     

Identification and Functional Analysis of the Caffeic Acid O-Methyltransferase (COMT) Gene Family in Rice (Oryza sativa L.)

Caffeic acid O-methyltransferase (COMT) is one of the core enzymes involved in lignin synthesis. However, there is no systematic study on the rice COMT gene family. We identified 33 COMT genes containing the methyltransferase-2 domain in the rice genome using bioinformatic methods and divided them into Group I (a and b) and Group II. Motifs, conserved domains, gene structure and SNPs density are related to the classification of OsCOMTs. The tandem phenomenon plays a key role in the expansion of OsCOMTs. The expression levels of fourteen and thirteen OsCOMTs increased or decreased under salt stress and drought stress, respectively. OsCOMTs showed higher expression levels in the stem. The lignin content of rice was measured in five stages; combined with the expression analysis of OsCOMTs and multiple sequence alignment, we found that OsCOMT8, OsCOMT9 and OsCOMT15 play a key role in the synthesis of lignin. Targeted miRNAs and gene ontology annotation revealed that OsCOMTs were involved in abiotic stress responses. Our study contributes to the analysis of the biological function of OsCOMTs, which may provide information for future rice breeding and editing of the rice genome.     

PGL-CIMS的设计与开发

本文介绍了平果铝业公司CIMS的系统结构、网络结构、软件结构、关键技术等一系列技术问题。     

Headquarter-centered common sourcing management through order coordination and consolidation

It is common for subsidiaries of a group company to use the same types of components for producing similar products. Different subsidiary companies may well procure such components from the same suppliers. This paper studies two sourcing management models. One is the Subsidiary-Autonomous Sourcing Management (SD-ASM) where subsidiaries manage their inventories and place purchasing orders independent of each other. The other is the Headquarter-centered Common Sourcing Management (HQ-CSM) where purchasing orders of subsidiaries are processed centrally through some kind of headquarter coordination. In the SD-ASM model, each subsidiary places replenishment orders at a time interval corresponding to their economic order quantity (EOQ). In the HQ-CSM model, two purchasing order management policies are examined. One is the Order Coordination policy in which common replenishment epochs or time periods are proposed by the headquarter and the subsidiaries are encouraged to coordinate the timing of their orders based on the common replenishment epochs. The other is the Order Consolidation policy in which the subsidiaries combine the quantity of their orders and the headquarter places a combined order with the supplier. In the Order Coordination policy, classic RAND heuristic is used to find the best common replenishment epoch and the best replenishment timing of each subsidiary. In the Order Consolidation policy, the optimal order quantity of the combined order is obtained from a mathematical model. The combined order is then allocated to the subsidiaries according to a proportional allocation rule. A series of numerical studies is conducted to compare the costs of the SD-ASM and HQ-CSM policies. The results show that HQ-CSM outperforms SD-ASM in terms of cost and robustness against demand uncertainties. This achievement is largely due to the economies of process (synergistic ordering process), the economies of scale (large order quantity with price discount) and risk pooling effect (transshipments). The results also reveal that the Order Consolidation policy with a combined order always performs better than the Order Coordination policy with common replenishment epochs especially in face of high demand uncertainties and high service level in the global market.     

一致性劈绕射理论在无源雷达绕射损耗中的应用研究

介绍了一致性劈绕射(UTD)的基本原理,推导了该理论下的一致性劈绕射场的分布表达式.以传统的劈绕射模型为基本参照,结合无源雷达电波传播特性,提出了以一致性劈绕射理论为基础的无源雷达绕射损耗模型,研究了有耗介质的无源雷达电波传播绕射损耗问题,并对该模型进行了仿真分析,为无源雷达电波传播遇到障碍物时电波的绕射损耗计算、分析、评估及应用提供了参考.     

浅述数据库的现状与未来

本文介绍了当前数据库技术的发展现状和流行的主流数据库,对数据库发展的新技术和未来的发展趋势也作了浅述.     

超声马达各向异性摩擦材料性能的试验研究

根据超声马达对摩擦材料切向和纵向性能的不同要求,采用在酚醛树脂胶中添加玻璃丝布和玻璃纤维增强的方法制备出不同弹性模量匹配的各向异性摩擦材料,并对其性能进行了测试。结果表明:当摩擦材料垂直方向与水平方向的弹性模量的比值为0.71~1.42时,摩擦材料受振动头的正压力和驱动力先增大后减小,比值为1.24时,马达的正压力和驱动力最大,驱动效果最好。     

服装材料悬垂伸长量与成衣样板修正量的研究

服装材料是成衣生产与制作的基本要素,其特性将直接影响成衣穿着的美观和舒适度,也影响成衣生产中工业样板的准确性.通过自制的模具(180°半圆模型)测量服装材料在悬垂状态下不同纱向、不同基础长度的悬垂伸长量,进行科学合理的量化分析,总结出服装材料悬垂伸长的规律.通过函数关系建立了服装材料悬垂伸长量和成衣样板修正量间的关系,为成衣样板的修正提供了科学的、可靠的依据,提高了成衣工业样板的制作效率和结构、规格的精度.     

面向生态的太子河流域水质水量联合调控研究

基于辽河流域河流生态需水量估算研究成果,提出了农业和生态用水耦合利用的河段水库群与闸坝联合调度模型,并制定了研究河段水质水量联合调控方案;调度方案在研究河段的有效实施,使干流水质得到改善,达到Ⅳ类水质标准;通过分析计算,当环境可利用水量为水4倍时,水质改善最为明显。