Measurement of caffeine in coffee beans with UV/vis spectrometer

In this research work using UV/vis spectrophotometer the molar decadic absorption coefficients and transitional dipole moment of pure caffeine in water and dichloromethane were obtained at 272 and 274.7 nm. The molar decadic absorption coefficients of caffeine in water and dichloromethane at these wavelengths are 1115 and 1010 m² mol⁻¹, respectively. The calculated values for the transitional dipole moment of caffeine in water and in dichloromethane are 10.40 × 10⁻³⁰ and 10.80 × 10⁻³⁰ C m, respectively. After characterizing caffeine in water and dichloromethane, fast and simple methods were developed that enable to quantify the content of caffeine in coffee beans. The methods helped in extracting caffeine from coffee dissolved in water by dichloromethane, and Gaussian fit was applied to eliminate the possible interference with the caffeine spectra.     

Studies on antigenic relatedness of classic and variant strains of infectious bursal disease viruses

Antigenic relatedness of six classic and variant strains of serotype 1 infectious bursal disease virus (IBDV) and one serotype 2 IBDV was investigated by Western blotting and enzyme-linked immunosorbent assay (ELISA) using polyclonal, monoclonal, and monospecific antibodies to single viral proteins (VP2 and VP3). All virus strains cross-reacted similarly, and the viruses were not distinguishable from each other by ELISA or Western blot analysis performed with polyclonal or non-neutralizing monoclonal and monospecific antibodies. Non-neutralizing antibodies against the VP2 (40 kilodaltons) reacted strongly with VP2 of classic and variant strains of serotype 1 and reacted weakly with VP2 of serotype 2 OH strain. This indicated that common antigens were recognized and that these epitopes were not strictly dependent on the native structure of the virus.     

Investigation of proper specimen geometry for mode I fracture toughness testing with flattened Brazilian disc method

Investigation of geometrical parameters for flattened Brazilian disc method is important, since this is a simple and attractive method for mode I fracture toughness testing on rock cores. Evaluating numerical modeling results, a parametric equation in terms of principal stresses at the center of the disc and the loading angle of the flattened end was developed. An equation was proposed for maximum stress intensity factors at critical crack lengths around stable to unstable crack propagation. Comparing fracture toughness results of flattened Brazilian disc method to the results of the suggested cracked chevron notched Brazilian disc method, geometrical parameters for flattened Brazilian discs were investigated. Diameter, loading angle of flattened ends, and thickness of andesite rock core specimens were changed to obtain comparable results to the suggested method. The closest results to the suggested method were obtained by 54 mm diameter discs with loading angles larger than 32°, and thicknesses between 19 and 34 mm. Results were confirmed by the flattened Brazilian disc tests on a marble rock. In flattened Brazilian disc tests with smaller loading angles and larger diameters, larger fracture toughness values than the results of the suggested cracked chevron notched were obtained. However, excluding tests with large loading angles over 27°; specimen size was less effective on the results of these tests. Critical crack length parameters computed from modeling and experiments were close to each other for the flattened Brazilian disc specimens with smaller loading angles around 20° and thickness/radius ratio equal or less than 1.1.     

Experimental Investigation of Aluminum Alloy and Steel Core Buckling Restrained Braces (BRBs)

International Journal of Steel Structures - Buckling restrained braces (BRBs) display balanced hysteretic behavior under reversed cyclic tension and compression forces and dissipate a significant...     

The impact of applying natural clinoptilolite (zeolite) on the chemical,sensory and microbiological changes of vacuum packed sardine fillets

Different doses (1% and 5%) of natural zeolite were applied to determine quality changes in vacuum packaged sardine fillets during 19 days at 4 ± 1 °C. Zeolite had an effect to improve sensory quality of sardine especially for removing off‐odour. The acceptable shelf life of vacuum packaged sardine was 8 days for control and 12 days for groups treated with 1% and 5% zeolite. The zeolite application resulted in significant reduction in total volatile basic nitrogen values, except for group treated with 5% zeolite at 15 days. Although the effect of zeolite depended on dose and specific storage days, application of zeolite had no effect on free fatty acid analysis. The use of zeolite significantly reduced ammonia and biogenic amine accumulation, especially for histamine and tyramine. The result of the study showed that the efficacy of zeolite as natural antimicrobials was high and lower dose of zeolite has to be applied to get maximum preservation effect.     

Experimental testing of a new solar dryer

Drying of agricultural products is an energy-intensive operation. High prices and shortages in fossil fuels increase the emphasis on using solar energy as an alternative energy source, especially in developing countries. In this respect, a new solar dryer, which consisted of a solar air heater and a drying chamber, was developed and used for drying various fruits and vegetables, i.e., sultana grapes, green beans, sweet peppers and chilli peppers and was successfully tested. In order to make a comparison, the traditional sun-drying experiments were employed. Results showed that the solar dryer reduced the drying time significantly and prevented mass losses, and essentially provided better product quality.     

Modification of polycaprolactone-styrene-vinyl trimethoxysilane terpolymer with sunflower oil for coating purposes

Polycaprolactone-styrene-vinyl trimethoxysilane terpolymer was prepared and then modified with sunflower oil partial glyceride (SFOPG) via a sol–gel method for use as a binder in coating applications. Therefore, a vinyl-functionalized polyester-based macromonomer (HPCL) was prepared via ring-opening polymerization of ?-caprolactone (CL) using 2-hydroxyethyl methacrylate (HEMA) followed by copolymerization with styrene (St) and vinyl trimethoxysilane (VTMS) to yield a terpolymer (HPCL-St-VTMS). The terpolymer was characterized by FTIR, GPC and ¹H NMR. To overcome the fact that the cured film of HPCL-St-VTMS was slightly soft, SFOPG was used as a modifier and was inserted to the structure via a sol–gel reaction between the hydroxyl groups and methoxy silane moieties of the terpolymer. After this modification, the softness disappeared. Nanosize inorganic domains were observed in the SEM image. Thermal analyses of the resulting cross-linked film of SFOM-HPCL-St-VTMS were performed with DSC and TGA. SFOM-HPCL-St-VTMS, which does not melt, resulted in a higher char yield and decomposition temperature at 5% weight loss compared to the unmodified HPCL-St-VTMS film. An evaluation of the film properties indicated that SFOM-HPCL-St-VTMS can be used as a coating material.