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991.
Araz Taeihagh Ren Baares-Alcntara Claire Millican 《Computers & Chemical Engineering》2009,33(10):1531
The formulation of policies requires the selection and configuration of effective and acceptable courses of action to reach explicit goals. A one-size-fits-all policy is unlikely to achieve the desired goals; as a result, the identification of a suite of alternative policies, together with clear indications of their trade-offs, is crucial to accommodate the diversity of stakeholders’ preferences. At present, the formulation of transport policies is done manually; this fact, together with the size of the space of possible policies, results in a large part of that space being left unexplored. A six-step framework to explore the space of alternative transport policies in order to achieve environmental targets is proposed. The process starts with a user-defined set of specific policy measures, using them as building blocks in the generation of alternative policy packages, clusters and future images according to the user's preferences and goals.The analysis framework is based on the visioning and backcasting approach used in the VIBAT report [Banister, D., & Hickman, R. (2006a). Visioning and backcasting for UK transport policy (VIBAT) project. Department for Transport's Horizons Research Programme 2004/06. The Bartlett school of planning and Halcrow Group Ltd. Retrieved 1/18/2008 http://www.ucl.ac.uk/ucft696/vibat2.html]. The framework is being implemented as a prototype decision support system around a case study: the formulation and analysis of policies required to achieve CO2 emission targets for the transport sector in the UK. Important insights on how to develop the framework have also been elicited from engineering design. The goal is to accelerate the task of policy-making and improve the effectiveness of the resulting policies.The proposed method and computer implementation is fundamentally different from the tools commonly used in the transport sector and is intended to assist (not replace) transport policy makers, and complement (not substitute nor compete with) existing mathematical modelling tools. This research constitutes the first step towards the development of a general family of computer-based systems that support the design of policies to achieve environmental targets—not only for transport, but also for other sectors such as energy and water. 相似文献
992.
Lin-Cereghino J Hashimoto MD Moy A Castelo J Orazem CC Kuo P Xiong S Gandhi V Hatae CT Chan A Lin-Cereghino GP 《Yeast (Chichester, England)》2008,25(4):293-299
The methylotrophic yeast, Pichia pastoris, is widely used as a host organism for the expression of heterologous proteins. Currently, the Zeocin and blasticidin resistance genes are the only dominant selectable markers that can be used for primary selection of transformants. In this report we describe new expression vectors that can be used to select directly for P. pastoris transformants using G418 resistance conferred by a modified Tn903kan(r) gene. Compared to other dominant markers, this system is more economical and offers a higher transformation efficiency, due to the small sizes of the cloning vectors, pKAN B and pKANalpha B (GenBank Accession Nos EU285585 and EU285586, respectively). Additionally, multicopy transformants can be generated using these new vectors. 相似文献
993.
This paper presents the analysis and design of a simple and effective switching frequency limitation process with sliding mode synthesis. The control scheme is based on self-oscillating phenomenon which naturally appears in relay feedback systems. Thanks to an analytical study, it shows that the maximum switching frequency of a sliding mode structure can always be limited by adding a simple second order filter in the feedback loop. To illustrate the implementation and the validity of this process, it is applied to a high dynamic voltage supply with a bandwidth of 2000 Hz and with a maximum switching frequency of 15 kHz. According to the experimental results, the switching frequency limitation process shows similar robustness properties to those of sliding mode control when the load and voltage supply change. 相似文献
994.
995.
Anna-Lena Neuheuser Claire Belo Rupert M. Bruckmaier 《Journal of dairy science》2017,100(10):8609-8613
In the present study we investigated the milking characteristics and the oxytocin release in dairy cows milked after either manual prestimulation or a premilking period with pulsating liners at normal pulsation rate (60) and ratio (60:40) while the pulsation chamber vacuum (PCV) was reduced to 20 kPa to prevent the opening of the liners. During the milking trial with 8 cows the PCV reduction was started either before attachment (PCV-1) or immediately after attachment (PCV-2) of the teat cups. Milk yields, total milking times, average milk flows, peak flow rates, the duration of milk flow plateaus, and the duration of milk flow declines did not differ among the 3 treatments. Only the time to reach peak milk flow was prolonged when the vacuum reduction was started after teat cup attachment (PCV-2). In this treatment, milk flow >200 g/min already occurred during the premilking period, resulting in bimodal milk flow curves. In 5 of the 8 cows, plasma oxytocin (OT) concentrations were measured from ?2 min before the start of milking until 3 min of milking to compare the OT release in response to manual prestimulation and during PCV-1. In both treatments, OT increased similarly and remained elevated until the end of measurements. Consequently, the areas under the curve of OT concentrations did not differ between treatments. In conclusion, milking performance is similar if milking is performed after manual prestimulation or after normal pulsation at reduced PCV. To prevent milk flow during the prestimulation period, it is of crucial importance to start the reduction of the PCV before cluster attachment. 相似文献
996.
There is an increasing need both to understand the translation of biomedical research into improved healthcare and to assess the range of wider impacts from health research such as improved health policies, health practices and healthcare. Conducting such assessments is complex and new methods are being sought. Our new approach involves several steps. First, we developed a qualitative citation analysis technique to apply to biomedical research in order to assess the contribution that individual papers made to further research. Second, using this method, we then proposed to trace the citations to the original research through a series of generations of citing papers. Third, we aimed eventually to assess the wider impacts of the various generations. This article describes our comprehensive literature search to inform the new technique. We searched various databases, specific bibliometrics journals and the bibliographies of key papers. After excluding irrelevant papers we reviewed those remaining for either general or specific details that could inform development of our new technique. Various characteristics of citations were identified that had been found to predict their importance to the citing paper including the citation??s location; number of citation occasions and whether the author(s) of the cited paper were named within the citing paper. We combined these objective characteristics with subjective approaches also identified from the literature search to develop a citation categorisation technique that would allow us to achieve the first of the steps above, i.e., being able routinely to assess the contribution that individual papers make to further research. 相似文献
997.
Lock CM Brust H van Breukelen M Dalmolen J Koeberg M Stoker DA 《Analytical chemistry》2012,84(11):4984-4992
The results of isotope ratio mass spectrometry (IRMS) on hexamethylene triperoxide diamine (HMTD) and its precursor hexamethylenetetramine (hexamine) is presented. HMTD was prepared from hexamine using several different sources of hexamine under both controlled laboratory conditions and in field experiments that represent the less controlled conditions that are likely to be observed in forensic casework scenarios. Precursor and product carbon isotope δ values consistently fit a linear relationship regardless of precursor or conditions. The magnitude of the isotope fractionation observed is affected by the efficiency of the reaction, with greater yielding reactions giving rise to HMTD with δ values more similar to the precursor material than lower yielding reactions. Nitrogen isotope δ values comparing precursor with product show some linearity when the reaction conditions are carefully controlled; however, results indicate a poor fit with linearity when synthesis conditions are more variable. Despite the greater variation, the HMTD product consistently has a more positive δ value compared with the hexamine precursor. The results observed from these experiments suggest hexamine reacts to form HMTD in a 1:1 ratio. Having prepared multiple HMTD samples from various precursors using a range of experimental conditions, we have observed results that may be useful in forensic investigations of improvised explosive materials. 相似文献
998.
Lorena Costa Garcia Leila Mendes Pereira Claire I. G. de Luca Sarantópoulos Miriam Dupas Hubinger 《Packaging Technology and Science》2012,25(7):413-425
Strawberry is a very sensitive fruit and presents a short post‐harvest shelf‐life. Among the factors responsible for strawberries' quality loss are high metabolic activities and mold decay. To increase product shelf‐life, cassava starch–based edible coatings (3%), added or not with potassium sorbate (0.05%), were applied on minimally processed strawberries. Uncoated minimally processed strawberries were used as control samples. Physical and chemical properties, respiration rate and sensorial acceptance of uncoated and coated strawberries stored up to 15 days at 5°C were monitored. The results showed that coatings had no significant effect on soluble solids, titrable acidity, pH and colour of strawberries. Besides reducing the respiration rate of samples, cassava starch edible coatings were efficient in delaying weight and firmness loss of strawberries during storage. In the studied conditions, potassium sorbate was not efficient in controlling microbial growth. Strawberries coated with cassava starch without the antimicrobial agent showed good conditions for consumption and good sensorial acceptance up to 12 days of storage, whereas the control samples and the samples treated with the coating containing potassium sorbate achieved a shelf‐life of 9 days because of microbial spoilage. Copyright © 2011 John Wiley & Sons, Ltd. 相似文献
999.
Yang X Tanaka Z Newhouse R Xu Q Chen B Chen S Zhang JZ Gu C 《The Review of scientific instruments》2010,81(12):123103
Two portable molecular sensing systems based on surface-enhanced Raman scattering (SERS) have been experimentally demonstrated using either a tip-coated multimode fiber (TCMMF) or a liquid core photonic crystal fiber (LCPCF) as the SERS probe. With Rhodamine 6G as a test molecule, the TCMMF-portable SERS system achieved 2-3 times better sensitivity than direct sampling (focusing the laser light directly into the sample without the fiber probe), and a highly sensitive LCPCF-portable SERS system reached a sensitivity up to 59 times that of direct sampling, comparable to the sensitivity enhancement achieved using fiber probes in the bulky Renishaw system. These fiber SERS probes integrated with a portable Raman spectrometer provide a promising scheme for a compact and flexible molecular sensing system with high sensitivity and portability. 相似文献
1000.
Joanne R. Hale Matthew J. Brookes Emma L. Hall Johanna M. Zumer Claire M. Stevenson Susan T. Francis Peter G. Morris 《Magma (New York, N.Y.)》2010,23(5-6):339-349