Evolution of carbohydrate fraction in carbonated fermented milks as affected by beta-galactosidase activity of starter strains

The influence of carbonation on the evolution of lactose, galactose and glucose in fermented milks with added probiotic bacteria (Lactobacillus casei, Lactobacillus acidophilus and/or Bifidobacterium bifidum) was evaluated and related to beta-galactosidase activity of starter strains. During incubation and first days of refrigeration, lactose hydrolysis resulting in the liberation of galactose and glucose occurred in CT (Streptococcus thermophilus/Lb. casei), AT (Str. thermophilus/Lb. acidophilus) and ABT fermented milks (Str. thermophilus/Lb. acidophilus/Bifid. bifidum). Levels of galactose were higher than those of glucose and could be related to the preferential consumption of glucose by actively growing bacteria. Through the incubation, lactose and monosaccharide levels were not affected by milk carbonation. However, during refrigerated storage the presence of this gas was associated with slightly lower content of lactose and higher levels of galactose and glucose in AT and ABT products but not in CT fermented milks. Through the refrigeration galactose was moderately utilised by Lb. acidophilus in AT products whereas the presence of Bifid. bifidum seems to prevent the consumption of this sugar in ABT fermented milks. Glucose remained constant, with minor variations in CT products but a continuous increase of this sugar occurred in carbonated AT and ABT fermented milks during storage. Beta-galactosidase activity displayed by Str. thermophilus strains was similar at pH 6.5 (initial pH of non-carbonated samples) and pH 6.3 (initial pH of carbonated samples) whereas Lb. acidophilus LaA3 showed greater beta-galactosidase activity at pH 6.3 than at higher pH values. Thus, the enhanced metabolic activity of Lb. acidophilus caused by the low initial pH of carbonated milk also promoted higher cellular beta-galactosidase activity that could have released greater amounts of galactose and glucose from lactose in AT and ABT fermented milks through the refrigerated period. In CT fermented milks, similar beta-galactosidase activity levels of Str. thermophilus at pH 6.5 and 6.3 together with the absence of beta-galactosidase activity in Lb. casei could explain the lack of differences on glucose and galactose content between carbonated and non-carbonated samples.     

Preferences of therapists-in-training for client characteristics.

The preferences of 132 therapists-in-training for 16 client characteristics were investigated using a paired comparison methodology. Therapists most preferred to work with clients who were psychologically minded, shared similar attitudes and values, and had dissimilar personally troubling problems. They least preferred to work with clients who were not psychologically minded, had dissimilar attitudes and values, and were older. Order of preferences was not influenced by therapist sex, race, clinical experience, socioeconomic status, sexual orientation, or theoretical orientation. Limitations of this study as well as implications for further research and training are discussed. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

Tagged Systems: A Framework for the Specification of History-dependent Properties

We propose a general method for the treatment of history-dependent runtime errors. When one has to control this kind of errors, a tagged version of the language is usually defined, in which tags capture only the necessary information of the history of processes. We will characterize such tagged languages as being quotients of the reachability tree defined by the computations of the original language. From this fact we can conclude that the property characterized by each tagged language is indeed a property of the original one. In this way, we can work in a common framework, instead of defining an ad hoc semantics for each property. In particular, we could still use the analysis machinery existing in the calculus in order to prove that or other related properties. We have applied this methodology to the study of resource access control in a distributed π-calculus, called Dπ. In particular, we have proved that the tagged version of Dπ is indeed a tagging according to our definition.     

Comparison of systematic search and database methods for constructing segments of protein structure

Two principal methods of determining the conformation of shortpieces of polypeptide backbone in proteins have been developed:using a database of known structures and systematicallygeneratingall conformations. In this paper, we compare the effectivenessof these two techniques. The completeness of the database forsegments of different lengths is examined and it is found tocontain most conformations for segments seven residues long,but to deteriorate rapidly for longer regions. When the databasesegment is to be incorporated into the rest of a structure,at least seven residues are required to build four new residues,because of the need to positionthe segment relative to the restof the structure.It is found that such positioning using flankingresidues results in large errors in the inserted region. Weconclude that the database method is currently not effectivefor comparative modeling, even for short segments. The systematicsearchprocedure is found to generate almost all structures of shortsegments found in proteinsIn contrast to the database method,low root mean square error structures are obtained for a setof trial segments embedded in the rest of a protein structure.Thus, it should be considered the method of choice.     

Life span changes in visual enumeration: The number discrimination task.

Participants from 5 groups with mean ages of 6, 8, 10, 22, and 72 yrs were tested on a series of speeded number discriminations: 1 vs 2, 3 vs 4, 6 vs 7, and 8 vs 9. The primary measure of interest (response time slope as a function of number size) decreased with age for numbers in the 1–4 range. However, a U-shaped age function emerged in the 6–9 range, with larger slopes for children and senior adults, and the smallest slopes for young adults. These data suggest that different processes are involved in enumerating small and large numbers of items. It is argued that subitizing, the process for small numbers, makes only minimal demands on spatial attention and thus shows developmental improvements without any decline in old age. In contrast, counting, the process for large numbers, requires sophisticated coordination of spatial attention, which has previously been shown first to improve and then decline over the life span. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Development of a system for categorizing client reactions to therapist interventions.

We developed a measure of client reactions to therapist interventions. The 21 categories of the measure were divided into 14 positive and 7 negative reactions, which differed significantly from each other on client helpfulness ratings. Preliminary validity data indicated that therapist intentions were related to client reactions more for successful cases than unsuccessful cases, pretreatment symptomatology was highly predictive of which reactions the clients reported, there were some predictable changes in reactions across time in treatment, and within-case correlations of reactions with client-rated session depth and smoothness indicated some similarities across cases. We discuss the case-specific nature of client reactions, methodological issues, and the need for greater therapist awareness of client reactions. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Beneath the surface of long-term therapy: Therapist and client report of their own and each other's covert processes.

Therapists and clients reported on their own and their perceptions of each other's covert processes in long-term therapy. Therapists had a match rate of .45 for client reactions, with therapists rating match on therapeutic work reactions as helpful. Although 65% of clients left somethimg unsaid (primarily because of avoidance), only 27% of therapists were able to match what clients left unsaid. Of the clients, 46% had secrets (often sexual), primarily because of shame or insecurity. Clients had a match rate of .50 for therapist intentions, with therapists rating match on exploratory and restructuring intentions as helpful and both clients and therapists rating match on assessment intentions as not helpful. Thus, awareness of the other's covert processes had an effect on therapy. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Therapist perspectives on countertransference: Qualitative data in search of a theory.

A consensual qualitative research strategy was used to examine data from 127 interviews conducted with 8 psychologists immediately following their sessions of brief therapy with 8 clients. Analyses revealed 3 domains relevant to countertransference: origins (including categories of family issues, needs and values, therapy specific issues, and cultural issues), triggers (including categories of content of client material, therapist comparing client with others, change in therapy structure or procedures, therapist assessing progress of therapy, therapist perception of client, and emotions), and manifestations (including categories of approach, avoidance, negative feelings, and treatment planning). The frequency of categories within and across cases was classified, and relationships among categories from the 3 domains were detected, generating hypotheses for future empirical research. Implications for practice, training, and continued research are discussed. (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Therapist retrospective recall impasses in long-term psychotherapy: A qualitative analysis.

Twelve experienced therapists completed a questionnaire, and 8 of the 12 were then interviewed about their experiences with a therapeutic impasse that ended in the termination of therapy. Data were analyzed using a qualitative methodology. Results indicated that most of the clients were anxious and depressed with personality disorders and interpersonal problems. Therapists perceived impasses as having a profound negative impact on both clients and therapists. Variables associated with impasses in a majority of the cases were a client history of interpersonal problems, a lack of agreement between therapists and clients about the tasks and goals of therapy, interference in the therapy by others, transference, possible therapist mistakes, and therapist personal issues. Implications for training, practice, and research are provided. (PsycINFO Database Record (c) 2010 APA, all rights reserved)