Experimental and model investigations of bleaching and saturation of fluorescence in flow cytometry

We investigated the fluorescence emission from three fluorophores commonly used for labeling cells in flow cytometry. We have demonstrated that the fluorescence emission from cells labeled with fluorescein-isothiocyanate (FITC), phycoerythrin (PE), and allophycocyanin (APC) is considerably saturated and bleached in standard flow cytometric conditions. Therefore, for optimization of fluorescence detection in a flow cytometer, it is important to know the emission kinetics in detail. We made a mathematical model of the optical processes involved: absorption, fluorescence emission, nonradiative decay, photodestruction, and triplet state occupation. The validity of the model was experimentally tested with a set of averaged fluorescence pulses, measured in a large range of intensities and illumination times. The fluorescence of APC could be completely described by the model and produced the following rate constants: photodestruction rate kb1 = 6 x 10(3) s(-1), triplet state population rate k12 = 2 x 10(5) s(-1), and depopulation rate k20 = 5 x 10(4) s(-1). The fluorescence kinetics of FITC- and PE-labeled cells could not be fitted with only three parameters over the entire range, indicating that other optical processes are involved. We used the model to determine the sensitivity of our flow cytometer and to calculate the optimum conditions for the detection of APC. The results show that in principle a single APC molecule on a cell can be detected in the presence of background, i.e., autofluorescence and Raman scattering by water.     

Mixture-proportioning of high-performance concrete

The paper presents a new approach to design concrete mixtures. It is based upon a set of models relating composition and engineering properties of concrete, to be implemented into software, linked with a material database. The principles underlying the various models are summarized, most of which focus on the granular structure of fresh/hardened concrete. A global approach to concrete is promoted, where performance specifications can be formulated in terms of fresh concrete (yield stress, plastic viscosity, slump and air content), hardening concrete (adiabatic temperature rise and autogenous shrinkage) and hardened concrete (compressive strength at any age, tensile strength, elastic modulus, creep and shrinkage). This approach is illustrated through the design of a special high-shrinkage high-performance concrete (HPC) for road application. To date, durability is lacking in the model and requires further research.     

Magnetic field effects on g-jitter induced flow and solute transport

Numerical modeling and analyses are presented of magnetic damping of g-jitter driven fluid flow and its effect on the solutal striation in a simplified Bridgman-Stockbarger crystal growth system under microgravity. The model development is based on the finite element solution of the momentum, energy and solute transport equations under g-jitter conditions in the presence of an external magnetic field. The numerical model is verified by comparison with analytical solutions obtained for a simple parallel plate channel flow driven by g-jitter in a transverse magnetic field. Simulations are carried out to study the behavior of convective flow and solutal transport induced by the combined g-jitter and magnetohydrodynamic forces. Both the idealized single frequency g-jitter force and the real g-jitter perturbation taken during space flight are considered. Results indicate that an applied magnetic field can effectively damp the velocity caused by g-jitter and help to reduce the time variation of solute redistribution. A stronger applied field is more effective in suppressing the convective flows and hence reducing concentration variation. It is found that g-jitter driven flows have the same oscillation period as the driving force with or without the applied field. However, an applied magnetic field shortens the transient period over which the flow field evolves into a quasi-steady state time harmonic oscillation after g-jitter sets in. The flow intensity increases with an increase in g-jitter magnitude but decreases with an increase in the applied field strength. The reduced convection in the liquid pool by the applied magnetic field results in a reduction of the concentration oscillation. The magnetic field is very useful in suppressing the spiking velocities that are induced by the spikes in the real g-jitter data. The damping effect is more pronounced if the magnetic field is switched on before the onset of g-jitter disturbances.     

Trypanosoma cruzi: nitrogenous-base-containing phosphatides in trypomastigote forms--isolation and chemical analysis

Increasingly clinicians attempt to base decisions regarding patient management on the results of clinical studies in addition to expert opinion and their own practical experience. In this article, the author reviews the published studies available to assist clinicians to make evidence-based decisions in three topics related to small volume red blood cell (RBC) transfusions for preterm infants; namely, studies examining the effects of RBC transfusions on possible symptoms of anemia such as tachypnea, apnea or other cardiorespiratory irregularities, studies investigating the collection and transfusion of umbilical cord blood and finally studies addressing the duration of storage and use of additive solutions for RBCs for transfusion to neonates. Based on the review of these studies, guidelines for small volume RBC transfusions in preterm infants are suggested.     

Bone marrow biopsy in the diagnosis of fever of unknown origin in patients with acquired immunodeficiency syndrome

BACKGROUND: Fever is commonly observed in patients with human immunodeficiency virus (HIV) disease and frequently eludes diagnosis. The role of bone marrow biopsy in the diagnosis of fever of unknown origin in patients infected with HIV remains controversial. PATIENTS AND METHODS: One hundred twenty-three consecutive patients with 137 episodes of fever lasting 10 or more days without diagnosis after 1 week of hospitalization were evaluated by bone marrow biopsy. RESULTS: Overall, a specific diagnosis was achieved in 52 episodes by means of culture and histopathological examination (diagnostic yield, 37.9%). Three types of disease were found: mycobacterial infections (n = 36, 69% of documented episodes), including 18 patients with disseminated tuberculosis and 14 with Mycobacterium avium-intracellulare complex infections; non-Hodgkin lymphomas (n = 12, 23%); and visceral leishmaniasis (n = 4, 8%). Although bone marrow cultures were more sensitive than microscopic examination with special stains for the diagnosis of mycobacterial infections, the pathological examination of bone marrow led to a more rapid diagnosis of disease. In addition, the histopathological examination of bone marrow alone led to the diagnosis of a specific condition in 43 episodes (31.3% of all episodes). CONCLUSIONS: Bone marrow biopsy is a useful procedure for the diagnosis of fever in patients with advanced HIV disease, particularly in areas where tuberculosis and leishmaniasis are prevalent. Involvement of the marrow may be the first indication of the existence of extranodal non-Hodgkin lymphoma. For Mycobacterium avium-intracellulare complex infection, blood cultures were more sensitive than bone marrow biopsy.     

Pseudo-cubic thin-plate-type spline method for analysing experimental data

This paper recalls the statistical origins of the minimum departure from nucleate boiling ratio (MDNBR) concept and Owen's coefficients, as used in CHF studies. The way in which they are currently used may be incorrect, and we shall present a new approach in which the lower tolerance interval limit depends on a number of parameters. The use of least-square regressions and smoothing splines is explained. We also include an example, based on a CHF EPRI test, from the Columbia University data bank.     

Using artificial intelligence to model heavy plate mill rolling

This research summary discusses the application of neural networks, evolutionary strategies, and other artificial intelligence techniques in the modeling and optimization of the models used for temperature, rolling force, and torque calculation in heavy plate milling.     

Psoriasis of the nails associated with disability in a large number of patients: results of a recent interview with 1,728 patients

We present the first case of esophagogastric devascularization and esophagogastric transection using a stapler through laparoscopic surgery. The procedure was performed in a 71-year-old diabetic woman with alcoholic liver cirrhosis (Child-Pugh B class), portal hypertension, bleeding grade III esophageal varices, and a previous bleeding episode. The surgical technique was carried out without problems, and the patient had an excellent postoperative condition. Esophagogastric devascularization with esophageal transection using a stapler through laparoscopic surgery is a feasible technique that accomplishes the same and all objectives of the open procedure. Operative time in both methods is the same, whereas surgical trauma, inmunologic depletion, amount of transfused blood, pain, use of analgesics, and hospital stay are reduced in the laparoscopic technique.