A decade of science and engineering of composite materials at the North West Composites Centre,University of Manchester,UK

The University of Manchester, School of Materials has a large multidisciplinary research programme on polymers, composites and carbon-based materials. This takes place through fundamental studies of structure-property relationships for these materials, including controlled synthesis and processing, and effects of structure andnano-, meso- and macro-scale morphology on physical properties and engineering applications.     

Nash equilibria: Complexity,symmetries, and approximation

We survey recent joint work with Christos Papadimitriou and Paul Goldberg on the computational complexity of Nash equilibria. We show that finding a Nash equilibrium in normal form games is computationally intractable, but in an unusual way. It does belong to the class NP; but Nash’s theorem, showing that a Nash equilibrium always exists, makes the possibility that it is also NP-complete rather unlikely. We show instead that the problem is as hard computationally as finding Brouwer fixed points, in a precise technical sense, giving rise to a new complexity class called PPAD. The existence of the Nash equilibrium was established via Brouwer’s fixed-point theorem; hence, we provide a computational converse to Nash’s theorem.To alleviate the negative implications of this result for the predictive power of the Nash equilibrium, it seems natural to study the complexity of approximate equilibria: an efficient approximation scheme would imply that players could in principle come arbitrarily close to a Nash equilibrium given enough time. We review recent work on computing approximate equilibria and conclude by studying how symmetries may affect the structure and approximation of Nash equilibria. Nash showed that every symmetric game has a symmetric equilibrium. We complement this theorem with a rich set of structural results for a broader, and more interesting class of games with symmetries, called anonymous games.     

Proof explanation for a nonmonotonic Semantic Web rules language

In this work, we present the design and implementation of a system for proof explanation in the Semantic Web, based on defeasible reasoning. Trust is a vital feature for Semantic Web. If users (humans and agents) are to use and integrate system answers, they must trust them. Thus, systems should be able to explain their actions, sources, and beliefs. Our system produces automatically proof explanations using a popular logic programming system (XSB), by interpreting the output from the proof’s trace and converting it into a meaningful representation. It also supports an XML representation for agent communication, which is a common scenario in the Semantic Web. In this paper, we present the design and implementation of the system, a RuleML language extension for the representation of a proof explanation, and we give some examples of the system. The system in essence implements a proof layer for nonmonotonic rules on the Semantic Web.     

A generalized Takagi–Sugeno–Kang recurrent fuzzy-neural filter for adaptive noise cancelation

This paper presents a recurrent fuzzy-neural filter for adaptive noise cancelation. The cancelation task is transformed to a system-identification problem, which is tackled by use of the dynamic neuron-based fuzzy neural network (DN-FNN). The fuzzy model is based on Takagi–Sugeno–Kang fuzzy rules, whose consequent parts consist of linear combinations of dynamic neurons. The orthogonal least squares method is employed to select the number of rules, along with the number and kind of dynamic neurons that participate in each rule. Extensive simulation results are given and performance comparison with a series of other dynamic fuzzy and neural models is conducted, underlining the effectiveness of the proposed filter and its superior performance over its competing rivals.     

Evaluation of the Role of p53 Tumour Suppressor Posttranslational Modifications and TTC5 Cofactor in Lung Cancer

Mutations in the p53 tumor suppressor are found in over 50% of cancers. p53 function is controlled through posttranslational modifications and cofactor interactions. In this study, we investigated the posttranslationally modified p53, including p53 acetylated at lysine 382 (K382), p53 phosphorylated at serine 46 (S46), and the p53 cofactor TTC5/STRAP (Tetratricopeptide repeat domain 5/ Stress-responsive activator of p300-TTC5) proteins in lung cancer. Immunohistochemical (IHC) analysis of lung cancer tissues from 250 patients was carried out and the results were correlated with clinicopathological features. Significant associations between total or modified p53 with a higher grade of the tumour and shorter overall survival (OS) probability were detected, suggesting that mutant and/or modified p53 acts as an oncoprotein in these patients. Acetylated at K382 p53 was predominantly nuclear in some samples and cytoplasmic in others. The localization of the K382 acetylated p53 was significantly associated with the gender and grade of the disease. The TTC5 protein levels were significantly associated with the grade, tumor size, and node involvement in a complex manner. SIRT1 expression was evaluated in 50 lung cancer patients and significant positive correlation was found with p53 S46 intensity, whereas negative TTC5 staining was associated with SIRT1 expression. Furthermore, p53 protein levels showed positive association with poor OS, whereas TTC5 protein levels showed positive association with better OS outcome. Overall, our results indicate that an analysis of p53 modified versions together with TTC5 expression, upon testing on a larger sample size of patients, could serve as useful prognostic factors or drug targets for lung cancer treatment.     

Effects of olive pomace and olive pomace oil on growth performance,fatty acid composition and cardio protective properties of gilthead sea bream (Sparus aurata) and sea bass (Dicentrarchus labrax)

Gilthead sea bream (Sparus aurata) and sea bass (Dicentrarchus labrax) were fed with two experimental diets: olive pomace diet and olive pomace oil diet, in order to examine whether fish oil substitution in fish feeds by olive oil production wastes, such as olive pomace and olive pomace oil, has an influence on growth performance, fatty acid composition and cardio protective properties of fish. Gilthead sea bream fed with both experimental diets exhibited satisfactory growth performance factors while sea bass did not. The total lipids of gilthead sea bream fed with olive pomace diet contained statistically decreased levels of fatty acids, while exhibited the most potent biological activity against platelet aggregation induced by Platelet Activating Factor. These data indicate that olive pomace can be used as a partial substitute of fish oil in fish feed improving its cardio protective properties.     

Dispersive liquid–liquid microextraction for the determination of organochlorine pesticides residues in honey by gas chromatography-electron capture and ion trap mass spectrometric detection

A simple dispersive liquid–liquid microextraction (DLLME) protocol for the determination of 15 organochlorine pesticides residues in honey is proposed. The selected pesticides were separated using gas chromatography and detected by electron capture (ECD) or ion trap mass spectrometry (GC-IT/MS). Several parameters affecting the extraction efficiency namely type and volume of organic extraction solvent, type and volume of disperser solvent, sample pH, ionic strength, extraction time and centrifugation speed were systematically investigated. The final DLLME protocol involved the addition of 750 μL acetonitrile (disperser) and 50 μL chloroform (extraction solvent) into a 5 mL aqueous honey solution followed by centrifugation. The sedimented organic phase (chloroform) were analysed directly by GC-IT/MS or evaporated and reconstituted in acetonitrile prior to the GC-ECD analysis. The analytical performance of the GC-ECD and GC-IT/MS methods was compared and discussed. Under the selected experimental conditions, the enrichment factors varied between of 36 and 114. The limits of detection (LOD) were in the range of 0.02–0.15 μg L⁻¹ (0.4–3 ng g⁻¹) for GC-ECD and 0.01–0.2 μg L⁻¹ (0.2–4 ng g⁻¹) for GC-IT/MS which is adequate to verify compliance of products to legal tolerances. The proposed method was applied to the analysis of the selected organochlorine pesticides residues in various honey samples obtained from Greek region. Mean recoveries were ranged from 75% to 119% while the precision was better than 20% in both methodologies.     

Charge transport mechanisms and memory effects in amorphous TaNx thin films

Amorphous semiconducting materials have unique electrical properties that may be beneficial in nanoelectronics, such as low leakage current, charge memory effects, and hysteresis functionality. However, electrical characteristics between different or neighboring regions in the same amorphous nanostructure may differ greatly. In this work, the bulk and surface local charge carrier transport properties of a-TaN_x amorphous thin films deposited in two different substrates are investigated by conductive atomic force microscopy. The nitride films are grown either on Au (100) or Si [100] substrates by pulsed laser deposition at 157 nm in nitrogen environment. For the a-TaN_x films deposited on Au, it is found that they display a negligible leakage current until a high bias voltage is reached. On the contrary, a much lower threshold voltage for the leakage current and a lower total resistance is observed for the a-TaN_x film deposited on the Si substrate. Furthermore, I-V characteristics of the a-TaN_x film deposited on Au show significant hysteresis effects for both polarities of bias voltage, while for the film deposited on Si hysteresis, effects appear only for positive bias voltage, suggesting that with the usage of the appropriate substrate, the a-TaN_x nanodomains may have potential use as charge memory devices.     

A structure-function-control paradigm for knowledge-based modeling and design of manufacturing workcells

This paper discusses the integration of structural, functional and control knowledge in manufacturing workcell modeling, simulation and design. After an overview of applications of semantic and object-oriented data models in the manufacturing domain, issues relating to the control synthesis for manufacturing workcells are presented. In particular, a data model encompassing functional and control features, along with application domain structural knowledge, is developed. This model assists in explicitly representing the control aspects of engineering design within an object-oriented database and supports a task-level, functionality-driven, manufacturing workcell design. Since manufacturing workcells consist of a number of elements interacting in a complex manner, workcell control design is one of the most difficult steps in the workcell design procedure. Message passage, commonly used in object-oriented databases, provides no explicit modeling of the database behavior. Hence, it can not serve as a tool for the design of system control. On the other hand, Petrinets (PN) have proven successful in describing complex interaction among active agents. This paper will explore the incorporation of Petri nets as a basis for describing application control knowledge within a structure-function-control data model.     

Development of a flow amperometric enzymatic method for the determination of total glucosinolates in real samples

The first amperometric flow analyzer, based on the biosensor concept, capable of determining total glucosinolates in real samples, is described. Myrosinase was immobilized on aminopropyl-modified controlled pore glass, which was then used for the construction of a packed-bed reactor. Myrosinase catalyzes the hydrolysis of glucosinolates (sinigrin) to glucose (among the other products), which is then oxidized by the action of glucose oxidase to produce hydrogen peroxide. The glucose enzyme electrode is based on a multimembrane architecture and was mounted on an amperometric flow cell (hydrogen peroxide detection at a platinum anode poised at +0.65 V vs Ag/AgCl/3M KCl). Different membrane types and different activation procedures were tested. The system was optimized to various working parameters, either as a glucose electrode or as a glucosinolate analyzer. The interference effect of various compounds was also investigated. Application of the method to real samples was carried out using glucose/glucose, hydrolyzed sinigrin and glucose/sinigrin solution as calibrators of the glucose electrode and the glucosinolate analyzer. Deviations due to the enantioselectivity of glucose oxidase to the beta-glucose anomer were observed, and a data elaboration protocol is proposed. The possibility of the simultaneous determination of glucose and glucosinolates is also demonstrated.