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排序方式: 共有202条查询结果,搜索用时 15 毫秒
21.
M Pintor JA Gómez L Ferrón CM Ferreirós MT Criado 《Canadian Metallurgical Quarterly》1998,47(9):757-760
Iron uptake analysis suggested that the Neisseria meningitidis transferrin (Tf) binding proteins, TbpA and TbpB, form only one type of receptor complex. Mutants defective in the synthesis of either TbpA or TbpB, but not defective in both proteins, can bind Tf, suggesting that both proteins are surface exposed and function in Tf binding. Also, iron uptake from Tf into the meningococci did not require the presence of both Tbps. The TbpB-defective mutant incorporated c. 37% of the iron taken up by the wild-type strain, but this was insufficient for bacterial growth. The TbpA-defective mutant incorporated c. 50% of the iron taken up by the wild-type strain and was able to grow with Tf as the only iron source. Mouse antibodies specific for TbpA were able to block c. 70% of the iron uptake from Tf in the wild-type strain, whereas they blocked only 22% of iron uptake in the TbpB-defective mutant and did not block uptake in the TbpA-defective strain. These results emphasise that TbpA should be considered in future vaccine trials in which iron-restricted proteins are to be included in the vaccine formulation. 相似文献
22.
Alessandro Luzio Francisco García Ferré Fabio Di Fonzo Mario Caironi 《Advanced functional materials》2014,24(12):1790-1798
Nanoscale hybrid dielectrics composed of an ultra‐thin polymeric low‐κ bottom layer and an ultra‐thin high‐κ oxide top layer, with high dielectric strength and capacitances up to 0.25 μFcm?2, compatible with low‐voltage, low‐power, organic electronic circuits are demonstrated. An efficient and reliable fabrication process, with 100% yield achieved on lab‐scale arrays, is demonstrated by means of pulsed laser deposition (PLD) for the fast growth of the oxide layer. With this strategy, high capacitance top gate (TG), n‐type and p‐type organic field effect transistors (OFETs) with high mobility, low leakage currents, and low subthreshold slopes are realized and employed in complementary‐like inverters, exhibiting ideal switching for supply voltages as low as 2 V. Importantly, the hybrid double‐layer allows for a neat decoupling between the need for a high capacitance, guaranteed by the nanoscale thickness of the double layer, and for an optimized semiconductor–dielectric interface, a crucial point in enabling high mobility OFETs, thanks to the low‐κ polymeric dielectric layer in direct contact with the polymer semiconductor. It is shown that such decoupling can be achieved already with a polymer dielectric as thin as 10 nm when the top oxide is deposited by PLD. This paves the way for a very versatile implementation of the proposed approach for the scaling of the operating voltages of TG OFETs with very low level of dielectric leakage currents to the fabrication of low‐voltage organic electronics with drastically reduced power consumption. 相似文献
23.
Mite species adapted to warm, humid climates are commonly found in house dust in the tropics. In Cuba, Dermatophagoides pteronyssinus, D. siboney, and Blomia tropicalis are the most common and abundant mite species in house dust. To investigate the pattern of sensitization of Cuban asthmatic patients to common mite species, we skin-prick-tested (SPT) 148 patients with a clinical history of asthma and possible mite allergy, and determined specific IgE antibodies against mite allergens (D. pteronyssinus, D. farinae, D. siboney, B. tropicalis, Acarus siro, Lepidoglyphus destructor, Tyrophagus putrescentiae, and Glycyphagus domesticus). The prevalence of positive SPT was high to D. siboney (88%), D. pteronyssinus (87%), A. siro (85%), B. tropicalis (85%), and D. farinae (83%). The largest skin reactions were obtained with D. siboney and B. tropicalis extracts. The skin test response to the D. siboney extract correlated to those of D. farinae, D. pteronyssinus, B. tropicalis, and A. siro. The highest IgE levels were found to Dermatophagoides species and B. tropicalis. IgE to D. siboney and B. tropicalis were found in 97% and 96% of the patients, respectively. The prevalence of specific IgE to the other mites studied varied from 46 to 65%. D. siboney and B. tropicalis are important sensitizers among asthmatic patients in Cuba. 相似文献
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25.
We have recently proposed a method, based on artificial neural networks (ANNs) to cluster protein sequences into families according to their degree of sequence similarity. The network was trained with an unsupervised learning algorithm, using, as inputs, matrix patterns derived from the bipeptide composition of the protein sequences. We describe here some further improvements to that approach. First, we propose a statistical method to cluster a set of bipeptidic matrices into families. It consists of three stages: (i) principal component analysis, (ii) determination of the optimal number M of clusters and (iii) final classification of the bipeptidic matrices into M clusters. Using a set of 444 protein sequences, we show that the classification given by the statistical method is in agreement with biological knowledge. We also show that the resulting classification is very similar to the one previously obtained with the ANN approach. Finally, we propose a new hybrid method of the statistical and ANN approaches, in which the results of the statistical method are used to choose the number of neurons and inputs of the network. We show that a network built in this way, and fed with a few principal components of the set of bipeptidic matrices as input signals, can be trained in an extremely short computing time. The resulting topological maps do not essentially differ from the ones obtained with the initial ANN approach. 相似文献
26.
27.
F Herencia A Ubeda ML Ferrándiz MC Terencio MJ Alcaraz M García-Carrascosa R Capaccioni M Payá 《Canadian Metallurgical Quarterly》1998,62(9):PL115-PL120
The effects of dichloromethane and methanol extracts from the marine invertebrates Leptogorgia ceratophyta, Holothuria tubulosa, Coscinasterias tenuispina and Phallusia fumigata on carrageenan-induced paw oedema in mice were investigated. The dichloromethane extract of Coscinasterias tenuispina and the methanol extract of Holothuria tubulosa administered p.o. at 50, 100 and 150 mg/kg, inhibited oedema in a dose-dependent manner 3 h after administration of carrageenan. Both extracts partially decreased elastase activity and PGE2 levels measured in homogenates from inflamed paws, without affecting the levels of this prostanoid present in stomach homogenates. As observed with the selective inhibitor NS398, both extracts can decrease cyclo-oxygenase activity in inflamed tissues but do not modify the constitutive cyclo-oxygenase enzyme. Therefore, these extracts represent new marine resources for the isolation of novel agents active on inflammatory conditions. 相似文献
28.
MT Delgado R Altisent C Gómez A Vitoria C Ferrández F Bielsa 《Canadian Metallurgical Quarterly》1993,11(4):170-173
OBJECTIVE: To assess the Primary Care doctor's performance when faced with his/her patients' alcohol consumption. DESIGN: Retrospective evaluation of data on procedures by means of explicit normative criteria and implicit check-up of those not complying. SETTING: Urban health centre. PATIENTS: A random and institutionally based sample, over a week, of patients of both sexes who came for a general medical consultation (eleven blocks) without prior appointment. RESULTS: 110 clinical histories were evaluated. 60% were not asked about alcohol consumption. In the cases of those who were asked, the amount consumed was specified. Excessive consumption was recorded in the notes as a health risk factor in 87%. A liver profile was requested for 62%. Advice to give up or reduce alcohol consumption was given in all cases. CONCLUSIONS: Poor compliance with the assessment criteria chosen justifies the implementation of the corrective measures proper to quality control methodology. We underline the importance of generalizing the implementation of the Programme of Preventive Activities in Primary Care and the need to improve health professionals' training and motivation on the question. 相似文献
29.
Manuel Acosta Daniel Coronado Esther Ferrándiz M. Dolores León Pedro J. Moreno 《Scientometrics》2017,112(1):215-240
This paper contributes to the literature on the factors explaining the regional university production of science and its quality in the field of Food Science and Technology (FS&T). We hypothesized that the regional quantity of science generated by universities is shaped not only by the amount of research and development (R&D) funds, as the main mainstream literature suggests, but also by the demand for science at the regional level. Furthermore, given the evolutionary nature of knowledge production, we suggest that the number of publications has a significant effect on the quality of scientific research at the regional level. Drawing on a sample of 48,207 scientific papers in FS&T over the period 1998–2010, we first map and examine the regional distribution of science and its quality across Europe-15. Second, we address our hypotheses by specifying several econometric models to identify the factors affecting the quantity and quality of scientific production. Our results show that the regional demand for FS&T—captured by the regional employment in the food and beverage industry—matters for the generation of science. Additionally, our findings support the hypothesis of a positive and significant effect of the production of papers on the scientific quality at the regional level. 相似文献
30.
Soulez F Denis L Fournier C Thiébaut E Goepfert C 《Journal of the Optical Society of America. A, Optics, image science, and vision》2007,24(4):1164-1171
We propose a microparticle localization scheme in digital holography. Most conventional digital holography methods are based on Fresnel transform and present several problems such as twin-image noise, border effects, and other effects. To avoid these difficulties, we propose an inverse-problem approach, which yields the optimal particle set that best models the observed hologram image. We resolve this global optimization problem by conventional particle detection followed by a local refinement for each particle. Results for both simulated and real digital holograms show strong improvement in the localization of the particles, particularly along the depth dimension. In our simulations, the position precision is > or =1 microm rms. Our results also show that the localization precision does not deteriorate for particles near the edge of the field of view. 相似文献