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221.
    
Advanced maternal age and obesity are the main risk factors to develop gestational diabetes mellitus (GDM). Obesity is a consequence of the increased storage of triacylglycerol (TG). Cytosolic and lysosomal lipid hydrolases break down TG and cholesteryl esters (CE) to release fatty acids (FA), free cholesterol, and glycerol. We have recently shown that intracellular lipases are present and active in the mouse placenta and that deficiency of lysosomal acid lipase alters placental and fetal lipid homeostasis. To date, intracellular lipid hydrolysis in GDM has been poorly studied despite the important role of FA in this condition. Therefore, we hypothesized that intracellular lipases are dysregulated in pregnancies complicated by maternal high-fat/high-cholesterol (HF/HCD) feeding with and without GDM. Placentae of HF/HCD-fed mice with and without GDM were more efficient, indicating increased nutrient transfer to the fetus. The increased activity of placental CE but not TG hydrolases in placentae of dams fed HF/HCD with or without GDM resulted in upregulated cholesterol export to the fetus and placental TG accumulation. Our results indicate that HF/HCD-induced dysregulation of placental lipid hydrolysis contributes to fetal hepatic lipid accumulation and possibly to fetal overgrowth, at least in mice.  相似文献   
222.
    
Hypothyroidism has been shown to reduce infarct size in rats, but the underlying mechanisms are unclear. We used isolated pressure-constant perfused hearts of control, hypothyroid and hyperthyroid mice and measured infarct size, functional parameters and phosphorylation of key molecules in cardioprotective signaling with matched heart rate. Compared with controls, hypothyroidism was cardioprotective, while hyperthyroidism was detrimental with enlarged infarct size. Next, we asked how thyroid hormone receptor α (TRα) affects ischemia/reperfusion (IR) injury. Thus, canonical and noncanonical TRα signaling was investigated in the hearts of (i) mice lacking TRα (TRα0), (ii) with a mutation in TRα DNA-binding domain (TRαGS) and (iii) in hyperthyroid TRα0 (TRα0hyper) and TRαGS mice (TRαGShyper). TRα0 mouse hearts were protected against IR injury. Furthermore, infarct size was reduced in the hearts of TRαGS mice that lack canonical TRα signaling but maintain noncanonical TRα action. Hyperthyroidism did not increase infarct size in TRα0 and TRαGS mouse hearts. These cardioprotective effects were not associated with increased phosphorylation of key proteins of RISK, SAFE and eNOS pathways. In summary, chronic hypothyroidism and the lack of canonical TRα signaling are cardioprotective in IR injury and protection is not due to favorable changes in hemodynamics.  相似文献   
223.
    
Cost-efficient deterministic method of moments solvers, as widely used to calculate average characteristics of chemical processes driven by population variations (e.g., average chain lengths), can be a posteriori extended with approximated solutions delivering distributed properties (e.g., chain length distributions). However, these solutions are rarely verified, specifically for complex systems with many population members and strong coupling, as is the case for industrially relevant free-radical-induced grafting (FRIG) toward graft copolymer (GC) synthesis with monomer unit dependent reactions. FRIG, as studied in the present work with polybutadiene at low styrene conversions, is an important chemical process, for example, the production of compatibilizers and high-impact materials. Deterministic model validation is uniquely performed by benchmarking the low to medium molar mass (MM) results (29 topologies) in the log-molar mass distribution with detailed matrix-based kinetic Monte Carlo simulation output, inherently capable of mapping distributions. The GC product is identified to be a heterogeneous mixture in MM, chemical composition, and molecular topology at any styrene conversion. The molecular structural evolution during GC synthesis is further theoretically related to both one-dimensional size-exclusion chromatography (1D-SEC) and two-dimensional liquid chromatography (2D-LC) analysis. It is shown that conventional SEC—even in the absence of broadening—is insufficient for GC separation, mainly due to the unavoidable coelution of topologically different GC species. In any case, the parallel running of advanced modeling tools allows for detailed molecular interpretation.  相似文献   
224.
225.
    
Although glass recycling is considered to be a default method for glass waste management, fine fractions of container soda-lime glass, or cullet of other compositions are still landfilled. This happens despite existing alternatives. Success could lie in advanced upcycled products that bring higher economic motivation for the implementation in industry, but these are often connected to alternative ways of product synthesis. We provide an example of waste glass upcycling by the preparation of glass microspheres (GM) from specialty low-sodium alumino borosilicate-based glasses via flame synthesis (FS). GM and the precursors, either from colorless medical vials or glass fibers, were characterized by scanning electron microscopy (SEM), simultaneous thermal analysis coupled with differential thermal analysis (STA-DTA), and image analysis. A dynamic corrosion test was performed and evaluated via ion-coupled plasma with optical emission spectroscopy (ICP-OES) to observe corrosion kinetics products. FS has proved to be a fast method of waste glass processing into GM. This article, besides the characterization of the starting material and final products, also suggests the possibility of processing for other landfilled waste glasses and also discusses the manufacturing of GM for water filters and fillers for polymers.  相似文献   
226.
The development of light-weight climbing robots capable of operating in various environments is a growing area in robotics. That is why there is a strong need for new tribologically-optimised materials at their gripping devices that may lead to the enhancement of the attachment force while reducing energy expenditure. Since insects are equipped with a set of very efficient attachment systems, enabling them to grip a variety of substrates, they may provide approaches for the innovation of climbing robots’ grippers. The goal of our study was a broad screening of polymeric materials to find out those with similar structure and functional principles to biological examples known from insect smooth attachment systems. Friction, adhesion and stiffness measurements were carried out. Rubber foamy materials covered with thin polymeric films and sandwich-like materials provided excellent compliant properties and the best performance in experiments (friction coefficients up to 3.2).  相似文献   
227.
Übersicht über die im Schrifttum aufgeführten Verfahren zur Zirkoniumbestimmung und deren Schwierigkeiten. Nacharbeitung der photometrischen Bestimmung mit Arsenazo III und Einsatz bei hohen Legierungsgehalten. Anwendung des Verfahrens auf geringere Zirkoniumgehalte ohne Vortrennung.  相似文献   
228.
The design of fiber-reinforced polymer reinforced concrete (FRP-RC) is typically governed by serviceability limit state requirements rather than ultimate limit state requirements as conventional reinforced concrete is. Thus, a method is needed that can predict the expected service load deflections of fiber-reinforced polymer (FRP) reinforced members with a reasonably high degree of accuracy. Nine methods of deflection calculation, including methods used in ACI 440.1R-03, and a proposed new formula in the next issue of this design guide, CSA S806-02 and ISIS M03-01, are compared to the experimental deflection of 197 beams and slabs tested by other investigators. These members are reinforced with aramid FRP, glass FRP, or carbon FRP bars, have different reinforcement ratios, geometric and material properties. All members were tested under monotonically applied load in four point bending configuration. The objective of the analysis in this paper is to determine a method of deflection calculation for FRP RC members, which is the most suitable for serviceability criteria. The analysis revealed that both the modulus of elasticity of FRP and the relative reinforcement ratio play an important role in the accuracy of the formulas.  相似文献   
229.
This paper describes the behavior of carbon fiber-reinforced polymer (CFRP) prestressed high-strength concrete prisms under direct tension. Seven prestressed concrete prisms with different levels of prestressing were cast and tested. Prisms were 50×50?mm in cross section and their lengths varied between 1,400 and 2,000?mm. Concrete compressive strength was as high as 147?MPa. Tension stiffening, crack width, and crack spacing in prisms were investigated. Concrete properties, such as the stress–strain relationship under direct tension and bond strength, were also determined. Test results revealed that tension stiffening in CFRP prestressed high-strength concrete is significant when higher concrete strength and higher prestressing level are applied. Tension stiffening factors are proposed based on the postcracking behavior of concrete. Experimental results also showed that increasing the prestressing level increases the amount of tension stiffening and reduces the number of cracks, which delays their appearance. However, cracks widened at a faster rate in the prisms with higher prestressing levels. Experimental results were compared with Comite Euro-International du Beton and American Concrete Institute proposed equations. Modifications were suggested for the above-mentioned equations to account for use of CFRP bars in prestressed sections.  相似文献   
230.
The effect of age and peroxidative stress on the concentration of a deoxyguanosine malondialdehyde adduct (dG-MDA) in rat tissues was investigated. Vitamin E deficiency had not effect on the dG-MDA content of liver DNA in rats fed a diet containing 10% corn oil. When 2% cod liver oil was added to this diet, the dG-MDA content of liver DNA doubled in the positive controls fed a high level of vitamin E (100 ppm dl-α-tocopherol), and there was a further increase when vitamin E was deleted. Neither iron nitrilotriacetate administration nor choline deficiency had any effect on the dG-MDA content of liver DNA. Carbon tetrachloride had a lowering effect. The failure of iron or carbon tetrachloride administration and of vitamin E deficiency to increase liver dG-MDA is consistent with their failure in previous experiments to affect the urinary excretion of dG-MDA. In contrast, these forms of peroxidative stress produce large increments in the urinary excretion of MDA adducts with lysine, reflecting increased formation and degradation of MDA-modified proteins. DNA appears to be protected from modification by MDA produced at extranuclear sites. The frequency of dG-MDA in different tissues of 4-month-old rats varied markedly: brain ≫ liver > kidneys and testes. Higher concentrations of dG-MDA were found in the liver and kidneys, but not the testes, of 25-month-old rats. The determinants of the concentration of dG-MDA in DNA merit further investigation.  相似文献   
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