How penultimate monomer unit effects and initiator influence ICAR ATRP of n‐butyl acrylate and methyl methacrylate

The relevance of penultimate monomer unit (PMU) effects and the selection of the correct initiator species under typical reversible deactivation radical copolymerization conditions is illustrated, using matrix‐based kinetic Monte Carlo simulations allowing the visualization of all monomer sequences along individual chains. Initiators for continuous activator regeneration atom transfer radical polymerization (ICAR ATRP) is selected as illustrative polymerization technique with n‐butyl acrylate and methyl methacrylate as comonomers, aiming at the synthesis of well‐defined gradient copolymers. Using literature based model parameters, in particular temperature dependent monomer and radical reactivity ratios, it is demonstrated that PMU effects on propagation and ATRP (de)activation cannot be ignored to identify the most suited ICAR ATRP reactants (e.g., tertiary ATRP initiator) and reaction conditions (e.g., feeding rates under fed‐batch conditions). The formulated insights highlight the need for further research on PMU effects on all reaction steps in radical polymerization. © 2017 American Institute of Chemical Engineers AIChE J, 2017     

Gewinnung von Antik?rpern gegen Sojaprotein aus Eiern immunisierter Legehennen

Zusammenfassung Die Autoren berichten über Erfahrungen mit der Immunisierung von Legehennen mit -, - und -Conglycinin aus Soja, und der Isolierung von spezifischen Antikörpern aus Eidottern. Die Aktivität und Spezifität der Immunglobuline wurde mittels Enzyme-linked immunosorbent assay (ELISA) untersucht. Besonders in Hinblick auf Einfachheit der Tierhaltung und Ausbeuten an Antikörpern bewährte sich diese Art der Antikörper-Gewinnung ausgesprochen gut. Auf die Notwendigkeit spezifischer Antiseren für die quantitative immunologische Bestimmung wird hingewiesen.

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Isolation of specific antibodies against soybean protein from the eggs of immunized laying hens

Summary The authors report on their experiences with immunization of laying hens with alpha-, alpha-and beta-conglycinin, and on the isolation of specific antibodies from the yolks. Activity and specifity of the immunoglobulins were tested by an enzyme-linked immuno sorbent assay (ELISA). This method of antibody production proved to be very convenient, especially in regard to the high yield of antibodies and the easy handling of the animals. The necessity of using specific antisera for quantitative immunoassays is pointed out.

     

Authenticity and Respect: Leading Creative Teams in the Performing Arts

Although leadership continues to play an important role in team effectiveness, how to lead teams of creative experts effectively remains a largely unanswered question. Within a performing arts context, this paper integrates the expert team and leadership literatures with the specific exigencies of the creative environment, particularly institutionalized theatre, and sheds new light on leadership and leadership behaviour in creative expert teams. The content analysis of semi‐structured narrative interviews from two theatre organizations in Austria and Germany reveals five leadership discourses not only on success and leadership, authenticity, respect, autonomy and freedom, but also on the dark side of leadership. The paper concludes by discussing findings and implications on leadership research with respect to the sensitivity of the creative context.     

Production of biogenic amines by lactic acid bacteria and bifidobacteria isolated from dairy products and beer

The aim was to monitor production of eight biogenic amines (BAs) (histamine, tyramine (TYR), tryptamine, putrescine, cadaverine (CAD), phenylethylamine, spermine and spermidine) by selected 81 lactic acid bacteria (LAB) strains: Lactobacillus, Lactococcus, Leuconostoc, Enterococcus, Pediococcus, Tetragenococcus and Bifidobacterium. The tested LAB and bifidobacteria were isolated from dairy products and beer. The decarboxylase activity of the micro‐organisms was studied in growth medium after cultivation. The activity was monitored by HPLC after the pre‐column derivatisation with dansylchloride. Fifty LAB showed decarboxylase activity. Thirty‐one strains produced low concentrations of CAD (≤10 mg L^?1). Almost 70% of beer isolates generated higher amounts of TYR (≤3000 mg L^?1). Most of the tested LAB demonstrated decarboxylase activity. The above micro‐organisms can contribute to the increase of content of BAs in dairy products or beer and thereby threaten food safety and health of consumers. Production of BAs even by the representatives of some probiotic strains (Bifidobacterium and Lactobacillus rhamnosus) was detected in this research. This study has also proved that contaminating LAB can act as sources of higher amounts of CAD and TYR in beer.     

On the benign qualities of behavioral disinhibition: Because of the prosocial nature of people, behavioral disinhibition can weaken pleasure with getting more than you deserve.

This article focuses on social situations in which people are surprised about what is happening and inhibited about how to respond to the situation at hand. We study these situations by examining a classic topic in social psychology: how people respond to receiving better outcomes than are deserved. In these situations, the actions of an authority or a coworker push in the direction of accepting and enjoying the unfair outcome, whereas personal values for most people push in the direction of rejecting or being displeased with the outcome. This conflict may inhibit people's response to the advantageous but unfair outcomes. If people are indeed inhibited about how to respond to these kinds of outcomes, then lowering behavioral inhibition by reminding people of having acted in the past without inhibitions (in a manner that is unrelated to the outcomes participants subsequently receive) should affect reactions to the outcomes. Specifically, we hypothesize that because many people are prosocial and want to adhere to principles of fairness, reminders of behavioral disinhibition will lead to less pleasure with the unfairly obtained outcomes. The results of 8 experiments (conducted both inside and outside the psychology laboratory) revealed evidence for this benign disinhibition effect on various reactions to outcomes that are better than deserved. In further accordance with our line of reasoning, the effect is particularly pronounced among those who adhere to a prosocial orientation or who have adopted a prosocial mindset and is not observed among those with proself orientations or mindsets. (PsycINFO Database Record (c) 2011 APA, all rights reserved)     

Microstructure-induced thermal stresses in pyrolytic carbon matrices at temperatures up to 2900 °C

Carbon/carbon composites produced by chemical vapor infiltration consist of carbon fibers embedded in a pyrolytic carbon matrix with a cylindrically layered structure at the microscale. Each coating layer has a different texture and different mechanical properties that depend on temperature. Stress distributions in such carbon matrices subjected to thermal loading and their possible failure scenarios are analyzed. A two-scale numerical model is developed. At the nanoscale, material properties of each layer are determined using a methodology based on the Eshelby's theory for continuously distributed inclusions. The resulting material parameters for each layer are then used in the finite element modeling at the microscale. Calculations are conducted for composites with different matrix structures for several cases of thermal loading. Calculated stress distributions show zones of maximal stress concentration and provide information on possible failure regions which correspond well with experimentally identified failure regions.     

MUG Mel3 Cell Lines Reflect Heterogeneity in Melanoma and Represent a Robust Model for Melanoma in Pregnancy

Melanomas are aggressive tumors with a high metastatic potential and an increasing incidence rate. They are known for their heterogeneity and propensity to easily develop therapy-resistance. Nowadays they are one of the most common cancers diagnosed during pregnancy. Due to the difficulty in balancing maternal needs and foetal safety, melanoma is challenging to treat. The aim of this study was to provide a potential model system for the study of melanoma in pregnancy and to illustrate melanoma heterogeneity. For this purpose, a pigmented and a non-pigmented section of a lymph node metastasis from a pregnant patient were cultured under different conditions and characterized in detail. All four culture conditions exhibited different phenotypic, genotypic as well as tumorigenic properties, and resulted in four newly established melanoma cell lines. To address treatment issues, especially in pregnant patients, the effect of synthetic human lactoferricin-derived peptides was tested successfully. These new BRAF-mutated MUG Mel3 cell lines represent a valuable model in melanoma heterogeneity and melanoma pregnancy research. Furthermore, treatment with anti-tumor peptides offers an alternative to conventionally used therapeutic options—especially during pregnancy.