Cyprinus carpio Skeleton Byproduct as a Source of Collagen for Gelatin Preparation

Byproducts obtained from fish processing account for up to 70% of their live weight and represent a large amount of unused raw materials rich in proteins, fats, minerals, and vitamins. Recently, the management of the use of predominantly cold-water fish byproducts has become a priority for many processing companies. This paper describes the biotechnological processing of byproducts of warm-water Cyprinus carpio skeletons into gelatins. A Taguchi experimental design with two process factors (HCl concentration during demineralization of the starting material and the amount of enzyme during enzyme conditioning of the collagen) examined at three levels (0.5, 1.0 and 2.0 wt%; 0.0, 0.1 and 0.2 wt% respectively) was used to optimize the processing of fish tissue into gelatin. Depending on the preparation conditions, four gelatin fractions were prepared by multi-stage extraction from the starting material with a total yield of 18.7–55.7%. Extensive characterization of the gel-forming and surface properties of the prepared gelatins was performed. Gelatins belong to the group of zero–low-medium Bloom value (0–170 Bloom) and low–medium viscosity (1.1–4.9 mPa·s) gelatins and are suitable for some food, pharmaceutical, and cosmetic applications. During processing, the pigment can be isolated; the remaining solid product can then be used in agriculture, and H₃PO₄Ca can be precipitated from the liquid byproduct after demineralization. The carp byproduct processing technology is environmentally friendly and meets the requirements of zero-waste technology.     

Replacement of Highly Conserved E222 by the Photostable Non‐photoconvertible Histidine in GFP

The widely used green fluorescent protein (GFP) decarboxylates upon irradiation; this involves removal of the acidic function of the glutamic acid at position 222, thereby resulting in the irreversible photoconversion of GFP. To suppress this phenomenon, the photostable, non‐photoconvertible histidine was introduced at position 222 in GFP. The variant E222H shows negligible photodynamic processes and high expression yield. In addition, the stable and bright fluorescence over a wide pH range makes the E222H protein an alternative for GFP in fluorescence imaging and spectroscopy. Other fluorescent proteins are predicted to benefit from replacement of the catalytic glutamic acid by histidine.     

New Approach for Estimating the Deflection of Beams Reinforced with FRP Reinforcement

Due to concerns with corrosion, the use of fiber-reinforced polymer (FRP) as a replacement to conventional steel reinforcement has greatly increased over the last decade. Researchers have identified the distinctive mechanical and bond properties of FRP reinforcement that prevent the use of existing relationships to establish serviceability of concrete structures reinforced with such products. Although studies have modified these empirical relationships to describe the behavior of structures reinforced with FRP reinforcement, this paper will provide a new approach to estimate deflection of concrete beams by considering material properties of the reinforcement and incorporating the effects of tension stiffening. Accuracy and precision of the approach was established by performing a statistical analysis on a database containing 171 FRP-reinforced concrete beams. Results were compared to those from existing proposed relationships and indicate the potential of the method to estimate deflection at various service conditions.     

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Localization of phosphatidylserine in boar sperm cell membranes during capacitation and acrosome reaction

One of the essential properties of mammalian, including sperm, plasma membranes is a stable transversal lipid asymmetry with the aminophospholipids, phosphatidylserine (PS) and phosphatidylethanolamine (PE), typically in the inner, cytoplasmic leaflet. The maintenance of this nonrandom lipid distribution is important for the homeostasis of the cell. To clarify the relevance of lipid asymmetry to sperm function, we have studied the localization of PS in boar sperm cell membranes. By using labeled annexin V as a marker for PS and propidium iodide (PI) as a stain for nonviable cells in conjunction with different methods (flow cytometry, fluorescence and electron microscopy), we have assessed the surface exposure of PS in viable cells during sperm genesis, that is, before and during capacitation as well as after acrosome reaction. An approach was set up to address also the presence of PS in the outer acrosome membrane. The results show that PS is localized in the cytoplasmic leaflet of the plasma membrane as well as on the outer acrosome membrane. Our results further indicate the cytoplasmic localization of PS in the postacrosomal region. During capacitation and acrosome reaction of spermatozoa, PS does not become exposed on the outer surface of the viable cells. Only in a subpopulation of PI-positive sperm cells does PS became accessible upon capacitation. The stable cytoplasmic localization of PS in the plasma membrane, as well as in the outer acrosome membrane, is assumed to be essential for a proper genesis of sperm cells during capacitation and acrosome reaction.     

Dynamic pull-in instability of a prestretched viscous dielectric elastomer under electric loading

The dynamic response of a pre-stretched elastomer membrane under electric loading was analysed. Thereby, two cases of voltage application, constant voltage and an incrementally increased voltage were regarded. The equation of motion (EOM) was derived from the Euler–Lagrange equation and the Rayleigh dissipation function. This allowed to include the influence of prestretch into the evolution equation of the viscous stretch. The critical values of pull-in instability under dynamic assumptions were determined at the initial state by an analytical model derived from the classical approaches of stability theory regarding geometric instabilities by using an energy criterion and were used to validate the numerical solution of the EOM. The results showed that both inertia and viscous effects have a notable influence on the pull-in stability behavior. In particular, at applied electric fields below the critical electric field the viscous behavior can still induce failure, which occurs time delayed. A non-monotonic dependence of the failure stretch on the magnitude of the applied electric fields below the critical value could be observed.     

Covalently Interlinked Graphene Sheets with Sulfur-Chains Enable Superior Lithium–Sulfur Battery Cathodes at Full-Mass Level

Sulfur represents a low‑cost, sustainable, and high theoretical capacity cathode material for lithium–sulfur batteries, which can meet the growing demand in portable power sources, such as in electric vehicles and mobile information technologies. However, the shuttling effect of the formed lithium polysulfides, as well as their low conductivity, compromise the electrochemical performance of lithium–sulfur cells. To tackle this challenge, a so far unexplored cathode, composed of sulfur covalently bonded directly on graphene is developed. This is achieved by leveraging the nucleophilicity of polysulfide chains, which react readily with the electrophilic centers in fluorographene, as experimental and theoretical data unveil. The reaction leads to the formation of carbon–sulfur covalent bonds and a particularly high sulfur content of 80 mass%. Owing to these features, the developed cathode exhibits excellent performance with only 5 mass% of conductive carbon additive, delivering very high full‑cathode‑mass capacities and rate capability, combined with superior cycling stability. In combination with a fluorinated ether as electrolyte additive, the capacity persists at ≈700 mAh g⁻¹ after 100 cycles at 0.1 C, and at ≈644 mAh g⁻¹ after 250 cycles at 0.2 C, keeping ≈470 mAh g⁻¹ even after 500 cycles.     

Cuticular Hydrocarbon Trails Released by Host Larvae Lose their Kairomonal Activity for Parasitoids by Solidification

Journal of Chemical Ecology - Successful host search by parasitic wasps is often mediated by host-associated chemical cues. The ectoparasitoid Holepyris sylvanidis is known to follow chemical...