Development of a Multi-Epitope Vaccine for Mycoplasma hyopneumoniae and Evaluation of Its Immune Responses in Mice and Piglets

Mycoplasma hyopneumoniae (Mhp), the primary pathogen causing Mycoplasma pneumonia of swine (MPS), brings massive economic losses worldwide. Genomic variability and post-translational protein modification can enhance the immune evasion of Mhp, which makes MPS prone to recurrent outbreaks on farms, even with vaccination or other treatments. The reverse vaccinology pipeline has been developed as an attractive potential method for vaccine development due to its high efficiency and applicability. In this study, a multi-epitope vaccine for Mhp was developed, and its immune responses were evaluated in mice and piglets. Genomic core proteins of Mhp were retrieved through pan-genome analysis, and four immunodominant antigens were screened by host homologous protein removal, membrane protein screening, and virulence factor identification. One immunodominant antigen, {"type":"entrez-protein","attrs":{"text":"AAV27984.1","term_id":"53987783","term_text":"AAV27984.1"}}AAV27984.1 (membrane nuclease), was expressed by E. coli and named rMhp597. For epitope prioritization, 35 B-cell-derived epitopes were identified from the four immunodominant antigens, and 10 MHC-I and 6 MHC-II binding epitopes were further identified. The MHC-I/II binding epitopes were merged and combined to produce recombinant proteins MhpMEV and MhpMEVC6His, which were used for animal immunization and structural analysis, respectively. Immunization of mice and piglets demonstrated that MhpMEV could induce humoral and cellular immune responses. The mouse serum antibodies could detect all 11 synthetic epitopes, and the piglet antiserum suppressed the nuclease activity of rMhp597. Moreover, piglet serum antibodies could also detect cultured Mhp strain 168. In summary, this study provides immunoassay results for a multi-epitope vaccine derived from the reverse vaccinology pipeline, and offers an alternative vaccine for MPS.     

装配夹具的设计及应用

设计一种能够使工件按一定的技术要求准确定位和牢固夹紧的杠杆夹具,提高产品质量和生产效率。     

对抗震钢框架板件宽厚比限值与相应的地震作用设计取值的细化和完善

在文献[1]的基本思路和初步结果的基础上,对钢结构中按弹性准则设计的截面,根据腹板屈曲与否对其进行分级;改进、完善和细化了框架截面等级与地震作用取值的对应关系,使之更趋合理;给出了各等级截面的框架以众值烈度地震作用效应表达的地震组合设计式,使实际应用更为方便。     

凯氏定氮仪在复混肥料总氮含量测定中的运用

我国是一个农业大国。农业的发展是国民经济和社会发展的重要因素。复混肥料在农业中的运用也日益广泛。文章研究的凯氏定氮仪法的检测方法与国标方法GB/T8572-2001的原理相同。与国标法比较,总氮含量的绝对误差在0.01%～0.29%之间,极差在0.07%～0.18%之间,检测准确度和精密度都符合国家标准的要求。此方法操作简便快速高效、安全可靠,效果令人满意。     

磷酸酯化淀粉的高效湿法合成研究

本研究选用磷酸盐组合物,进行在低温下的淀粉高效磷酸酯化反应。结果表明,在湿法的低温条件下,采用适当的复合磷酸盐及反应促进体系,体系中淀粉浓度在40%～42%,磷酸氢二钠和磷酸二氢钠的比例为0.5,复合盐用量15%,pH保持在5～6之间,50℃反应5 h,Gx16在1%～5%的用量,可以显著提高淀粉的磷酸酯化程度(以结合磷含量表示),同时由于反应效率的提高,使湿法的反应均匀性,残留磷酸盐含量可控性,流程简洁、方便等优势得以突出,对比现实的生产工艺,该方法在稳定质量、节能降耗、减少污染排放等方面都极具优势,有重要的推广价值。     

化工原理及其实验教学改革

为了适应化学工业新技术日新月异的发展,根据化工原理及化工原理实验课程的特点以及化工学科今后的发展趋势对化工原理及其实验课程教学进行教学改革,以提高学生对该课程的理解、独立实验操作能力和实验创新能力,促进课程教学质量的提高。     

新型耐盐耐溶剂水包油型高分子乳化剂C-5的合成及应用研究

以失水山梨醇单油酸酯(Span-80)作为乳化剂,偶氮二异丁基脒盐酸盐作为聚合引发剂,以丙烯酸(AA)、丙烯酰胺(AM)、二甲基二烯丙基氯化铵(DADMAC)和特殊单体为反应物,通过反相乳液聚合法合成了一种新型水包油型高分子乳化剂C-5,并考察了这种乳化剂的耐盐耐溶剂性能.