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61.
Watanabe T. Sakaida N. Yasaka H. Kano F. Koga M. 《Lightwave Technology, Journal of》2000,18(8):1069-1077
We examine the fiber transmission performance of the optical signal whose chirp is controlled by utilizing phase modulation in semiconductor optical amplifier (SOA) with both simulations and experiments. This chirp control technique converts a positive chirp created by electroabsorption (EA) modulator into negative chirp, which reduces the waveform degradation due to the chromatic dispersion in transmission over standard single-mode fiber (SMF). It also provides an optical gain that is sufficient to compensate the insertion loss of the EA modulator. We investigate how the chirp control is affected by the input power to the SOA and the carrier lifetime of the SOA. As the SOA input power increases, the negative chirp becomes large, while the waveform is largely distorted due to gain saturation. However, the waveform distortion at high SOA input powers can be shaped by using a frequency discriminator. The acceleration of the carrier lifetime also reduces the waveform distortion due to gain saturation. We demonstrate that the chirp control technique is effective even for a high bit rate optical signal up to 10 Gb/s, when the carrier lifetime is expedited by optical pumping 相似文献
62.
We established a label-free method of measuring proteins in crude cell lysate using antibody arrays and surface plasmon resonance (SPR) imaging. The refractivity of the running buffer was adjusted with that of the lysate to overcome the bulk effect. The chemistries of the fabricated arrays were investigated to reduce nonspecific adsorption on the array surface. We found that the hydrophilicity of the poly(ethylene glycol) moiety and lower electrostatic charge on the surface provided a specific measurement of antigen-antibody interaction. We validated the system by measuring the expression of eight proteins in the mouse brain and comparing the results to those by conventional Western blotting. The detection limit of the antibody array was approximately 30 ng/mL in crude cell lysate, on the same order as that of previous SPR research. The system enabled quick, label-free, and high-throughput analysis of abundant proteins with minimal sample volume ( approximately 200 muL). It is expected that our SPR antibody array will be applicable for direct protein expression profiling of cell lysate, as well as for cell phenotyping, food analysis, discovery of new biomarkers, and immunological disease diagnostics. 相似文献
63.
Sato H. Wada T. Ohbayashi S. Kozaru K. Okamoto Y. Higashide Y. Shimizu T. Maki Y. Morimoto R. Otoi H. Koga T. Honda H. Taniguchi M. Arita Y. Shiomi T. 《Solid-State Circuits, IEEE Journal of》1999,34(11):1571-1579
This paper describes a 0.25-μm, 64 K×36 bit pipelined burst SRAM using a 6.156-μm2 cell. It realizes over 500-MHz operation using a lower cost double metal process, Internal 16 K×144 organization by T-shaped bit line array reduces 20% of latency, 20% of active power, and 8.5% of die size. The low power also enables us to use lower cost thin quad flat type packages. Our solution to the soft error problem, a shallow triple well structure and four-transistor cell with stacked capacitor, improved soft error rate by 3.5 orders of magnitude compared with the conventional SRAM cell 相似文献
64.
65.
Oligodeoxynucleotides possessing a shortened internucleotidic linkage have been prepared using carbocyclic nucleosides lacking the 5'-methylene as the monomeric units. Specifically, the oligomers derived from carbocyclic 5'-n or thymidine and 2'-hydroxy-3'-deoxythymidine will be described. 相似文献
66.
Aisawa S. Watanabe A. Goh T. Takigawa Y. Koga M. Takahashi H. 《Communications Magazine, IEEE》2003,41(9):54-57
This article highlights advances in NTT's optical path crossconnect systems that use planar-lightwave circuit switches. A photonic MPLS router that can handle up to 256 optical label switched paths is developed as one result of our R&D activities; mature OPXC technologies are adopted to create a practical OPXC system. 相似文献
67.
68.
In this study, the theoretical worst and the average interchannel crosstalk are shown as a function of normalized input power. The validity of normalization by saturation input power is confirmed by experimental data. This normalized input power must be set below -20 and -10 dB for multiwavelength numbers of 20 and 4, respectively, to attain an average interchannel crosstalk of under -10 dB. The optical loss, including interchannel crosstalk, that can be compensated by a traveling-wave-type semiconductor laser amplifier (TWA) is analyzed. The experimental results indicate that the compensable optical loss could be evaluated by using the average interchannel crosstalk in multiwavelength transmission system design. Applying a TWA as a preamplifier is more effective in systems that include excess optical loss in the receiver 相似文献
69.
1. The in vitro and in vivo metabolism of 2,4,5,2',4',5'-hexachlorobiphenyl (PCB153) in guinea pig has been studied. 2. Seven metabolites were detected in the faeces of PCB153-treated animals and three were identical to those produced by dog liver microsomes. The detection of a metabolite where a chlorine atom was shifted from the 2- to 3-position strongly suggested the involvement of 2,3-arene oxide intermediate, and evidence for the concomitant formation of a 3,4-arene oxide intermediate was provided by identifying other two minor metabolites which were dechlorinated at the 4-position. 3. In vitro studies using liver microsomes from guinea pigs revealed that the 2,3-arene oxide and 3-hydroxylation pathways are the predominant metabolic routes compared with the 3,4-arene oxide pathway. Although the guinea pig is an another species that can metabolize PCB153 mainly to the 2,3-arene oxide intermediate, the rate of formation was only about one-tenth of the dog. 4. These results indicate that the ability to form this unusual 2,3-arene oxide intermediate may not be responsible for high excretion rate of this congener. Our data also suggest that the cytochrome P450-catalysed metabolism of PCB153 in the guinea pig and dog are similar, whereas for post-cytochrome P450 metabolism, the guinea pig resembles the rabbit. 相似文献
70.
This paper presents a control design for the one‐phase Stefan problem under actuator delay via a backstepping method. The Stefan problem represents a liquid‐solid phase change phenomenon which describes the time evolution of a material's temperature profile and the interface position. The actuator delay is modeled by a first‐order hyperbolic partial differential equation (PDE), resulting in a cascaded transport‐diffusion PDE system defined on a time‐varying spatial domain described by an ordinary differential equation (ODE). Two nonlinear backstepping transformations are utilized for the control design. The setpoint restriction is given to guarantee a physical constraint on the proposed controller for the melting process. This constraint ensures the exponential convergence of the moving interface to a setpoint and the exponential stability of the temperature equilibrium profile and the delayed controller in the norm. Furthermore, robustness analysis with respect to the delay mismatch between the plant and the controller is studied, which provides analogous results to the exact compensation by restricting the control gain. 相似文献