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Konecni Vladimir; Ebbeson Ebbe B.; Konecni Daiva K. 《Canadian Metallurgical Quarterly》1976,61(3):359
Examined the relationship between the drivers' distance from an intersection (0-100 yds) when the light changed from green to yellow and the probability that the drivers would proceed through the intersection. 100 men and 44 women comprised the experiment sample; 77 men and 29 women formed a control group. The function relating the 2 variables approximated a normal ogive, but there was additional evidence that drivers took both distance and speed into account in deciding whether to proceed or stop. Among the drivers who were at intermediate distances (40-60 yds) when the light changed, younger males (estimated to be under 30) were more likely both to proceed and to violate the red light than were other drivers. The latter finding could be attributed to the younger males' faster driving and the related tendency to ignore the consequences of the decision conflict induced by the yellow light. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
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Jevgenija Jakubovska Dr. Daiva Tauraitė Prof. Dr. Rolandas Meškys 《Chembiochem : a European journal of chemical biology》2019,20(19):2504-2512
A set of five N4-acyl-modified 2′-deoxycytidine 5′-triphosphates were incorporated into modified DNA by using phi29 DNA polymerase, and cleavage by selected restriction endonucleases was studied. Modified DNA containing N4-acyl functional groups in either one or both strands of a DNA molecule was resistant to the majority of restriction enzymes tested, whereas modifications outside of the recognition sequences were well tolerated. The N4-acylated cytidine derivatives were subjected to competitive nucleotide incorporation by using phi29 DNA polymerase, showing that a high-fidelity phi29 DNA polymerase efficiently used the modified analogues in the presence of its natural counterpart. These N4 modifications were also demonstrated to be easily removed in an aqueous ethanolamine solution, in which all steps, including primer extension, demodification, and cleavage by restriction endonuclease, could be performed in a one-pot procedure that eliminated additional purification stages. It is suggested that N4-modified nucleotides are promising building blocks for a programmable; transient; and, most importantly, straightforward DNA protection against specific endonucleases. 相似文献
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Greta Švermickaitė Viktorija Eisinaitė Rimantė Vinauskienė Ina Jasutienė Daiva Leskauskaitė 《International Journal of Food Science & Technology》2022,57(7):4441-4450
In this study, aerogels were prepared from kognac glucomannan (KG) or whey proteins loaded with bioactive sea buckthorn pomace extract. KG was diacetylated with Na2CO3 (0.1; 0.2; 0.3; 0.4 m ) resulting hydrogel formation that were further freeze-dried to obtain an aerogel structure. Whey protein aerogels were prepared by removing pore fluid from alcogels using supercritical CO2 drying. Produced aerogels evaluated for microstructure, porosity, specific surface area, absorption capacity, encapsulation efficiency and antioxidant capacity of the extract. It was found that higher concentration of alkali induced higher hardness, resilience and elastic modulus values. It was also obtained that pores in the konjac glucomannan aerogels were irregular in shape and a decrease in total pore volume (0.026 to 0.019 cc/g) and surface area (12.39 to 11.40 m2/g) after increasing the carbonate concentration was observed. These aerogels were found to have better encapsulation efficiency properties for sea buckthorn pomace extract (17 to 20%) in comparison to whey protein aerogels (0.05 to 0.36%). Overall, the KG aerogels show potential for applications in the food industry as a carrier of bioactive sea buckthorn pomace extract, while whey proteins must be used in combination with other biopolymers to enhance their bioactive compound loading capacity. 相似文献
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Rokas Miksiunas Ruta Aldonyte Agne Vailionyte Tadas Jelinskas Romuald Eimont Gintare Stankeviciene Vytautas Cepla Ramunas Valiokas Kestutis Rucinskas Vilius Janusauskas Siegfried Labeit Daiva Bironaite 《International journal of molecular sciences》2021,22(23)
Dilated cardiomyopathy (DCM) is the most common type of nonischemic cardiomyopathy characterized by left ventricular or biventricular dilation and impaired contraction leading to heart failure and even patients’ death. Therefore, it is important to search for new cardiac tissue regenerating tools. Human mesenchymal stem/stromal cells (hmMSCs) were isolated from post-surgery healthy and DCM myocardial biopsies and their differentiation to the cardiomyogenic direction has been investigated in vitro. Dilated hmMSCs were slightly bigger in size, grew slower, but had almost the same levels of MSC-typical surface markers as healthy hmMSCs. Histone deacetylase (HDAC) activity in dilated hmMSCs was 1.5-fold higher than in healthy ones, which was suppressed by class I and II HDAC inhibitor suberoylanilide hydroxamic acid (SAHA) showing activation of cardiomyogenic differentiation-related genes alpha-cardiac actin (ACTC1) and cardiac troponin T (TNNT2). Both types of hmMSCs cultivated on collagen I hydrogels with hyaluronic acid (HA) or 2-methacryloyloxyethyl phosphorylcholine (MPC) and exposed to SAHA significantly downregulated focal adhesion kinase (PTK2) and activated ACTC1 and TNNT2. Longitudinal cultivation of dilated hmMSC also upregulated alpha-cardiac actin. Thus, HDAC inhibitor SAHA, in combination with collagen I-based hydrogels, can tilt the dilated myocardium hmMSC toward cardiomyogenic direction in vitro with further possible therapeutic application in vivo. 相似文献