Activity of caprylic acid, carvacrol, ε-polylysine and their combinations against Salmonella in not-ready-to-eat surface-browned, frozen, breaded chicken products

Caprylic acid (CAA), carvacrol (CAR), ε-polylysine (POL), and their combinations were evaluated for reduction of Salmonella contamination in not-ready-to-eat surface-browned, frozen, breaded chicken products. Fresh chicken breast meat pieces (5 × 5 × 5 cm) were inoculated with Salmonella (7-strain mixture; 4-5 log CFU/g) and mixed with distilled water (control) or with CAA, CAR, and POL as single or combination treatments of 2 or 3 ingredients. Sodium chloride (1.2%) and sodium tripolyphosphate (0.3%) were added to all formulations, followed by grinding of the mixtures and forming into 9 × 5 × 3 cm portions. Sample surfaces were brushed with egg whites, coated with breadcrumbs, surface-browned in an oven (208 °C, 15 min), packaged, and stored at -20 °C (7 d). Total reductions of inoculated Salmonella in untreated (control) surface-browned, breaded products after frozen storage were 0.8 to 1.4 log CFU/g. In comparison, single treatments of CAA (0.25% to 1.0%), CAR (0.3% to 0.5%), and POL (0.125% to 1.0%) reduced counts by 2.9 to at least 4.5, 3.4 to at least 4.4, and 1.4 to 2.3 log CFU/g, respectively, depending on concentration. Pathogen counts of products treated with 2- or 3-ingredient combination treatments (0.03125% to 0.25% CAA, 0.0375% to 0.3% CAR, and/or 0.5% POL) were 0.4 to at least 3.3 log CFU/g lower (depending on treatment) than those of the untreated controls. The antimicrobial activity of 2-ingredient combinations comprised of 0.125% CAA, 0.15% CAR, or 0.5% POL was enhanced (P < 0.05) when applied as a 3-ingredient combination (that is, 0.125% CAA + 0.15% CAR + 0.5% POL). These data may be useful for the selection of antimicrobial treatments to reduce Salmonella contamination in not-ready-to-eat processed chicken products. PRACTICAL APPLICATION: Findings from the study may be useful for the selection of suitable antimicrobials, concentrations, and combinations to reduce Salmonella contamination in not-ready-to-eat surface-browned, frozen, breaded chicken products.     

Mating sequence, dominance and paternity success in captive male tammar wallabies

The tammar wallaby (Macropus eugenii) is a small, promiscuous, macropodid marsupial. Females usually produce a single young each year and there is a clear dominance hierarchy between adult males. The dominant male usually mates first and then guards the female to prevent access to her by other males. In this study, agonistic encounters and mating behaviour were observed to determine male dominance hierarchies in six groups of captive tammars consisting of a total of 23 males and 50 females. Mating behaviour was observed immediately post-partum when females were in oestrus and was correlated with plasma testosterone concentrations. Male mating sequences were recorded, and the paternity of offspring was determined by using seven macropodid marsupial microsatellites. Rates of sexual checking and aggression by males housed with females in oestrus in the non-breeding season were lower than in the breeding season. These males also had lower concentrations of testosterone, but were still able to sire young. High testosterone concentrations neither ensured dominance nor appeared to control directly the level of sexual activity. Females usually mated with more than one male. The dominant male most often secured the initial copulation (60%), but the first-mating male did not always secure parentage, with second and third matings resulting in as many young as first matings. Using these data, we were unable to discount first sire, last sire or equal chance models of paternity in this species. Half the young (50%) were sired by the dominant alpha male, but of the remaining progeny, the beta male sired more (35%) than gamma and delta males (15%). Dominance therefore is only a moderately effective predictor of paternity in the tammar. Although the dominant males gained most first matings and individually sired half of the offspring, the subdominant males still contributed significantly to the population, at least in captivity.     

Pulsed field gel electrophoresis typing of human and retail foodstuff Campylobacters: an Irish perspective

Campylobacter enteritis is a zoonosis, an infectious disease transmissible under normal conditions from vertebrate animals to man, presenting a major global public health burden. In this study, Pulsed Field Gel Electrophoresis (PFGE) was employed to identify common genotypes in a collection of 600 Campylobacter isolates in order to investigate if profiles obtained from retail samples of foodstuffs matched genotypes causing illness in the community in Ireland. The Campylobacters were isolated from retail foodstuffs, and cases of gastroenteritis, over the same 20-month period in three population centres in Ireland. The major observation made was of a high level of PFGE-genotype heterogeneity; 236 SmaI discrete genotypes were found in 507 strains successfully analysed. Analysis of the PFGE profiles revealed 22 common profiles amongst food isolates and those causing enteritis in humans. These cojoint PFGE genotypes indicate that 56 (38%) of the human clinical isolates are genetically related to 129 (36%) of the food isolates. The identification of these recurrent PFGE types, in the sampled Campylobacter coli and Campylobacter jejuni populations, indicates that a high proportion of Campylobacter isolates found in foods of animal origin also occur in patients with symptoms of enteritis. This data adds weight to the epidemiological hypothesis that a high proportion of human Campylobacter cases are contracted via the handling and consumption of contaminated foodstuffs, in particular poultry.     

Visualizing biochemical activities in living cells through chemistry

The development of molecular probes to visualize cellular processes is an important challenge in chemical biology. One possibility to create such cellular indicators is based on the selective labeling of proteins with synthetic probes in living cells. Over the last years, our laboratory has developed different labeling approaches for monitoring protein activity and for localizing synthetic probes inside living cells. In this article, we review two of these labeling approaches, the SNAP-tag and CLIP-tag technologies, and their use for studying cellular processes.     

Object Touch by a Humanoid Robot Avatar Induces Haptic Sensation in the Real Hand

Humanoid robot embodiment is a recently developed form of mediated embodiment. In 2 studies, we report and quantify a new haptic (touch) illusion during embodiment of a humanoid robot. Around 60% of the users in our studies reported haptic sensations in their real hand when they observed their robot avatar touching a curtain with its hand. Critically, our study shows for the first time that users can experience haptic sensations from a nonanthropomorphic embodied limb/agent with visual feedback alone (i.e. no haptic feedback provided). The results have important implications for the understanding of the cognitive processes governing mediated embodiment and the design of avatar scenarios.     

Simulation of hydrogen adsorption systems adopting the flow through cooling concept

Hydrogen storage systems based on adsorbent materials have the potential of achieving the U.S. Department of Energy (DOE) targets, especially in terms of gravimetric capacity (the 2017 DOE target for the system weight capacity is 5.5%). This paper deals with analysis of adsorption storage systems adopting the flow through cooling concept. By this approach the feeding hydrogen provides the needed cold to maintain the tank at low temperatures. Two adsorption systems have been examined and modeled adopting the Dubinin–Astakhov model, to see their performance under selected operating conditions. A first case has been analyzed, modeling a storage tank filled with carbon based material (namely MaxSorb^®) and comparing the numerical outcomes with the available experimental results for a 2.5 L tank. Under selected operating conditions (minimum inlet hydrogen temperature of approximately 100 K and maximum pressure on the order of 8.5 MPa) and adopting the flow through cooling concept the material shows a gravimetric capacity of about 5.7%. A second case has been modeled, examining the same tank filled with metal organic framework material (MOF5^®) under approximately the same conditions. The model shows that the latter material can achieve a (material) gravimetric capacity on the order of 11%, making the system potentially able to achieve the DOE 2017 target.     

Teaching uncertainty propagation as a core component in process engineering statistics

Propagation of uncertainty refers to evaluation of uncertainty in output(s) given uncertainty in input(s). This can be across a physical process, or can be predicted based on a process model. Uncertainty can be propagated analytically, by application of Taylor series variance propagation, or numerically, through repeated Monte-Carlo simulations. Propagation of uncertainty is an important concept in process engineering statistics, which is not currently widely taught. In this paper, an approach is provided for teaching uncertainty propagation as part of a larger process engineering statistics course, applying analytical and numerical propagation principles, including consideration of correlation in inputs. A saline blending practical is used as a case study, with experimental and theoretical determination of how variability in feed pump flows determines variability in outlet conductivity. Based on a class of 132 2nd year Chemical Engineering students, learning outcomes in analytical and numerical linear and non-linear propagation models can be attained and enhanced applicability and engagement within the core statistics course. An engagement survey particularly noted that the students recognised the importance of propagation as a technical capability, but noted difficulties in linking the experimental work to theory of propagation. Overall, propagation of uncertainty allows educators to increase the direct relevance of statistics to process engineering and engage with students through their existing analytical capabilities.     

GM food and neophobia: connecting with the gatekeepers of consumer choice

Resistance to importation of genetically modified (GM) foods in rich countries has deterred governments in many food‐exporting countries from approving the planting of GM food crops for fear of damaging export markets for conventional food. Apart from governments that have actively imposed barriers to entry, another level of resistance can arise from food distribution channel members deciding not to import foods which they believe consumers in their markets will not want. GM foods fall in this category in some markets, particularly in Europe. In China and India, the two most populous consumer markets, pragmatic considerations appear likely to overcome neophobia regarding this technology, provided that benefits are adequately communicated to consumers. Choice‐modelling experiments show that the same may well be true in Europe. Copyright © 2008 Society of Chemical Industry