A Novel Nanoparticle‐Based Disposable Electrochemical Immunosensor for Diagnosis of Exposure to Toxic Organophosphorus Agents

A novel disposable electrochemical immunosensor for highly selective and sensitive detection of organophosphorylated butyrylcholinesterase (OP‐BChE), a specific biomarker for exposure to toxic organophosphorus agents, is presented. In this new approach, zirconia nanoparticles (ZrO₂) were employed to selectively capture the OP moiety of OP‐BChE adducts, followed by quantum dot (QD)‐tagged anti‐BChE antibodies for amplified quantification. The captured CdSe‐QD tags can be sensitively detected by stripping voltammetry using an in situ bismuth‐plating method. The OP agent, diisopropylfluorophosphate (DFP), was selected to prepare OP‐BChE adducts in various matrices. The formation of OP‐BChE adducts in plasma sample was confirmed using mass spectroscopy. The developed electrochemical immunosensor demonstrates a highly linear voltammetric response over the range of 0.1 to 30 nM OP‐BChE, with a detection limit of 0.03 nM (based on signal/noise = 3), coupled with a good reproducibility (relative standard deviation 4.5%). Moreover, the immunosensor has been validated with biomonitoring of OP‐BChE adducts in the plasma samples. This novel nanoparticle‐based electrochemical immunosensor thus provides an alternative way for designing a sensitive and cost‐effective sensing platform for on‐site screening/evaluating exposure to a variety of OP agents.     

巯基乙酰壳聚糖制备条件的优化及其絮凝性能

通过正交试验研究了巯基乙酰壳聚糖(MAC)的最佳制备条件,探讨了MAC捕集重金属离子的机理,研究了pH、浊度对MAC除铜效果的影响,并对MAC去除重金属离子Cu2+、Cd2+、Ni2+的效果进行了对比。结果表明:制备MAC的最佳条件为:m(壳聚糖)∶m(巯基乙酸)为1∶2,pH为5.0,反应时间为3.5 h;水样浊度对铜离子的去除有促进作用;优化条件下,MAC对重金属离子的选择性顺序为Cd2+Cu2+Ni2+。     

The effect of roll-processing orientation on stress corrosion cracking of warm-rolled 304L stainless steel in oxygenated and deoxygenated high temperature pure water

Stress corrosion crack growth rates of 304L stainless steel after one-directionally warm-rolled along the longitudinal (L) direction were measured in oxygenated and deoxygenated pure water at 288 °C. The fracture mode is mainly intergranular in the Transverse-longitudinal (T-L) orientation specimen, while it is occasionally intergranular in the Longitudinal-transverse (L-T) orientation specimen. The crack growth rates in the T-L orientation are higher than those in the L-T orientation in both oxygenated and deoxygenated environments, which is related to the effective cracking growth path as the result of microstructural anisotropy and applied loading direction.     

中海壳牌公司在检修工作中的HSE管理

介绍壳牌公司在HSE管理上所做的工作、管理水平、对承包商的要求及认识到清洗公司今后在清洗施工及HSE管理上要努力的方向。     

Experimental study on high viscosity fluid micro-droplet jetting system

Fluid dispensing is a method by which fluid materials(such as epoxy,adhesive,and encapsulant) are delivered in a controlled manner in electronics packaging.Fluid jetting,derived from inkjet technology,is a noncontact,data-driven fluid dispensing technology.But ideal fluid materials for packaging are usually high viscous,which is difficult to realize by traditional inkjet technology.In this paper,a mechanical micro-droplet jetting system for high viscosity fluid was proposed.It consists of dispensing valve,m...     

强碱性阴离子交换树脂污染原因分析及复苏工艺研究

对神华国华神木发电有限公司1号、2号阴床用强碱性阴离子交换树脂进行检测分析,指出其污染物主要为有机物和三价铁离子,通过实验筛选了经济合理的复苏方案。结果证明:采用压缩空气擦洗、反洗除去杂质,分离破碎树脂,用10%盐酸溶液浸泡除铁,用8%NaCl+4%NaOH+0.1 mL/L乳化剂op-10配制复苏溶液加热浸泡除有机物,可获得较好的复苏效果。     

一种新型光标记交换技术

光交换技术是光网络中的一项重要技术。文章介绍了一种新的光标记交换技术,利用光载波的抑制来产生光标记和载荷,并对新旧标记替换方法进行了对比,采用新技术所产生的标记和载荷具有很高的消光比,相互之间没有串扰,在接收端容易分离,且标记和载荷部分可以具有不同码型和协议,灵活性更强。     

基于AlGaN/GaN HEMT的C波段混合集成功率合成放大器的设计

研制了一种基于AlGaN/GaN HEMT的功率合成技术的混合集成放大器电路.该电路包含4个10×120 μm的HEMT晶体管以及一个Wilkinson功率合成器和分配器.在偏置条件为VDS=40V,IDS=0.9A时,输出连续波饱和功率在5.4GHz达到41.4dBm,最大的PAE为32.54%,并且功率合成效率达到69%.     

温差电致冷元件损伤层的测试方案

提出一种间接测量损伤层厚度的方法,此方法具有较高的精度。     

Image aesthetic quality evaluation using convolution neural network embedded learning

A way of embedded learning convolution neural network (ELCNN) based on the image content is proposed to evaluate the image aesthetic quality in this paper. Our approach can not only solve the problem of small-scale data but also score the image aesthetic quality. First, we chose Alexnet and VGG_S to compare for confirming which is more suitable for this image aesthetic quality evaluation task. Second, to further boost the image aesthetic quality classification performance, we employ the image content to train aesthetic quality classification models. But the training samples become smaller and only using once fine-tuning cannot make full use of the small-scale data set. Third, to solve the problem in second step, a way of using twice fine-tuning continually based on the aesthetic quality label and content label respective is proposed, the classification probability of the trained CNN models is used to evaluate the image aesthetic quality. The experiments are carried on the small-scale data set of Photo Quality. The experiment results show that the classification accuracy rates of our approach are higher than the existing image aesthetic quality evaluation approaches.