The furanosesterterpene variabilin was identified in an unpalatable crude extract and proved to be a feeding deterrent when offered at 0.23% of artificial diet dry weight to reef fishes in field assays. The icthyodeterrent property of variabilin was expressed when food pellets for the palatability assays were made with calcium alginate, but not carrageenan. Variabilin probably failed to show deterrent activity in the carageenan matrix because of decomposition during the necessary heat treatment. 相似文献
This paper considers the relationship between the adoption of electronic traceability systems (ETSs) and the organization of firms. More precisely, it analyzes the respective roles of a firm's organizational structure, and organizational changes, in the process of ETS adoption in agribusiness. We use data from the French “Organizational Changes and Computerization” survey from 2006. We test a probit model to demonstrate the organizational structure and organizational changes underlying the firm's ETS adoption choice. Results show that ETS adoption is strongly favored by organizations with heavy hierarchical structures, standardized managerial practices and contractual mechanisms with external partners. This adoption process seems to coevolve with the organization: firms that implemented an ETS during the observed period (2003–2006) have experienced the most important organizational changes in terms of managerial practices, information systems and contractual relations, as well as the strengthening of the intermediate levels in the hierarchy. 相似文献
Many enzymes involved in xenobiotic metabolism, including cytochrome P450 (CYP) 1A1, are regulated by the aryl hydrocarbon receptor (AhR). 3,3'',4,4'',5-Pentachlorobiphenyl (PCB 126) is a potent ligand for AhR and can thus induce the expression of CYP1A1. Interestingly, we observed that human carcinoma cell lines derived from different types of epithelial cells displayed divergent degrees of CYP1A1 induction after exposure to PCB 126. Since epigenetic mechanisms are known to be involved in cell type-specific gene expression, we sought to assess the epigenetic determinants of CYP1A1 induction in these carcinoma cell lines. In contrast to HepG2 hepatocarcinoma cells, HeLa cervical carcinoma cells showed significantly lower levels of CYP1A1 mRNA expression following PCB 126 exposure. Our results show that the two cell lines maintained differences in the chromatin architecture along the CYP1A1 promoter region. Furthermore, treatment with the epigenetic modifiers, trichostatin A (TSA) and 5-aza-2''-deoxycytidine (5-Aza-dC), significantly increased the expression of CYP1A1 after PCB 126 treatment in HeLa cells. However, we did not observe apparent differences in methylation levels or specific location of CpG DNA methylation between the two cell lines in the analyzed CYP1A1 promoter region. Taken together, our findings suggest that the differences in CYP1A1 expression between HepG2 and HeLa cells are due to differences in the chromatin architecture of the CYP1A1 promoter and thus establish a role of epigenetic regulation in cell-specific CYP1A1 expression. 相似文献
As a consequence of organophosphorus (OP) toxins posing a threat to human life globally, organophosphorus hydrolase (OPH) has become the enzyme of choice to detoxify such compounds. Organophosphorus hydrolase was covalently immobilized onto a quartz substrate for utilization in paraoxon detection. The substrate was cleaned and modified prior to chemical attachment. Each modification step was monitored by imaging ellipsometry as the thickness increased with each modification step. The chemically attached OPH was labeled with a fluorescent dye (7-isothiocyanato-4-methylcoumarin) for the detection of paraoxon in aqueous solution, ranging from 10(-9) to 10(-5) M. UV-visible spectra were also acquired for the determination of the hydrolysis product of para-oxon, namely p-nitrophenol. 相似文献
A study of inclusion complexation of liquid non-ionic surfactants, nonylphenol (NP) and nonylphenol 9 mole ethoxylate (NP9EO), with β-cyclodextrin (β-CD), was carried out by mass spectrometry, surface tension, and ultraviolet-visible (UV-VIS) and Fourier transform infrared (FTIR) spectroscopies. The inclusion complexation was effectuated by heating at 80 °C and filtration of aqueous NP + β-CD and NP9EO + β-CD suspensions. The mass spectrometry and surface tension measurements revealed that NP and NP9EO form inclusion complexes with β-CD and β-CD possesses a higher affinity for NP. These results are supported by the data from UV-VIS spectroscopic analyses that have indicated that a three times greater amount of NP is entrapped into β-CD than NP9EO. This phenomenon has been associated with the smaller size and a higher degree of hydrophobicity of NP that favours its entrapment into β-CD as compared to that of NP9EO. At the structural level, the data from FTIR spectroscopic study have indicated that alkyl chains of NP and NP9EO can form van der Waals interactions with the cavity of β-CD. Moreover, NP and NP9EO seem to cause a reorganization of the intramolecular hydrogen bonds and change of the hydration of β-CD, but did not appear to strongly interact with C-C, C-O-C, and OH groups of β-CD. Together these results suggest that the formation of inclusion complexes by NP and NP9EO with β-CD molecules could constitute an effective and advantageous technique to remove liquid non-ionic surfactants from wastewater due to the non-toxic character of β-CD to humans and the environment. 相似文献
Artificial synapses for femtomolar detection : Amperometry at platinized carbon fibre electrodes has been used to unravel the complexity of β‐lapachone's effects on cellular oxidative stress. α‐Lapachone, the pharmacologically inactive para‐quinone isomer, did not display such characteristics, but over longer incubation periods both quinones induced apoptosis. The observed effects were interpreted in terms of two mechanisms involving opposite reactivities of quinones in living cells.
Brettanomyces spp. is an indigenous yeast that can grow during wine fermentation and impact its flavor. The characteristic flavor is often described as phenolic/medicinal, spices, cloves, earthy, barnyard, and horsy. The acceptability of Brettanoymces-induced flavors depends on the flavor intensity, personal preference, and consumer expectations. The yeast has been isolated from grapes, barrels, and winery equipment. It impacts wines from all the top producing regions in the world, with associated flavors most often found in red wines. Prevention of growth of this yeast in wine involves attention to fruit quality and winery sanitation, control of sulfite and oxygen levels, and the use uncontaminated barrels. Greater understanding of how Brettanomyces and its metabolites contribute to wine flavor is required to recognize its impact on consumer acceptability. 相似文献