Kinetics and design relation for enzymatic conversion of lactose into galacto-oligosaccharides using commercial grade β-galactosidase

The enzymatic synthesis of galacto-oligosaccharides (GOS) from lactose was studied using commercial grade β-galactosidase (Biolacta FN5) from Bacillus circulans. The reaction was carried out under free enzyme condition varying initial lactose concentration (ILC: 55-525?g/L), enzyme concentration (0.05-1.575?g/L), temperature (30-50°C) and pH (5.0-6.0). Reaction mixture compositions were analyzed utilizing high performance liquid chromatography (HPLC). A?maximum GOS formation of 39% (dry basis) was achieved at an ILC of 525?g/L converting 60% of the lactose fed. Tri-saccharides were the major types of GOS formed, accounting approximately 24%; whereas, tetra-saccharides and penta-saccharides account approximately 12% and 3%, respectively. Design correlation was developed in order to observe the quantitative effect of operating parameters on GOS yield. Further, based on Michaelis-Menten model, four-step reaction pathways were considered for simplistic understanding of the kinetics. Apart from predicting the reaction mixture composition, the approach also provided kinetic parameters though simulation using COPASI 4.7(?). Excellent agreements were observed between simulated and experimental results.     

Antibacterial activities of polyethylene glycol, tween 80 and sodium dodecyl sulphate coated silver nanoparticles in normal and multi-drug resistant bacteria

Antibacterial activity of silver nanoparticles coated with different functionalizing agents i.e., polyethylene glycol, tween 80 and sodium dodecyl sulphate were evaluated on both normal and multi-drug resistant strains of bacteria. Under the same reaction conditions, these functionalizing agents were added separately to coat silver nanoparticles. Among these, polyethylene glycol coated nanoparticles were most effective in killing all the bacterial strains which includes Escherichia coli DH5a, Bacillus subtilis, Micrococcus luteus, Staphylococcus aureus and multi-drug resistant clinical isolates of Shigella spp. (flexneri, boydii, sohnea) and Vibrio cholerae. The minimum inhibitory concentration of polyethylene glycol coated silver nanoparticles was also less compared to the other two sets of nanoparticles. Consistence with that polyethylene glycol coated nanoparticles produced more intracellular reactive oxygen species in bacteria. Moreover, when human cell lines MCF7 and Chang Liver were incubated in presence of these nanoparticles for 18 h with same concentrations as used for bacteria, no toxicity was observed. But significant increase in cell killing was observed with longer incubation time. Thus our present investigation implicates the potential therapeutic use of silver nanoparticles as antibacterial agent particularly the polyethylene glycol coated one.     

Graphene as a nanocarrier for tamoxifen induces apoptosis in transformed cancer cell lines of different origins

A cationic amphiphile, cholest-5en-3β-oxyethyl pyridinium bromide (PY(+) -Chol), is able to efficiently disperse exfoliated graphene (GR) in water by the physical adsorption of PY(+) -Chol on the surface of GR to form stable, dark aqueous suspensions at room temperature. The GR-PY(+) -Chol suspension can then be used to solubilize Tamoxifen Citrate (TmC), a breast cancer drug, in water. The resulting TmC-GR-PY(+) -Chol is stable for a long time without any precipitation. Fluorescence emission and UV absorption spectra indicate the existence of noncovalent interactions between TmC, GR, and PY(+) -Chol in these suspensions. Electron microscopy shows the existence of segregated GR sheets and TmC 'ribbons' in the composite suspensions. Atomic force microscopy indicates the presence of 'extended' structures of GR-PY(+) -Chol, which grows wider in the presence of TmC. The slow time-dependent release of TmC is noticed in a reconstituted cell culture medium, a property useful as a drug carrier. TmC-GR-PY(+) -Chol selectively enhanced the cell death (apoptosis) of the transformed cancer cells compared to normal cells. This potency is found to be true for a wide range of transformed cancer cells viz. HeLa, A549, ras oncogene-transformed NIH3T3, HepG2, MDA-MB231, MCF-7, and HEK293T compared to the normal cell HEK293 in vitro. Confocal microscopy confirmed the high efficiency of TmC-GR-PY(+) -Chol in delivering the drug to the cells, compared to the suspensions devoid of GR.     

Design, fabrication, and evaluation of a multilevel spiral-phase Fresnel zone plate for optical trapping

A compact optics configuration for the generation of donut beams for trapping atoms at the micrometer scale using a multilevel spiral-phase Fresnel zone plate (FZP) and a semiconductor laser is proposed. A FZP is designed and a multilevel spiral phase is integrated into it. A spiral-phase FZP with a radius of 1?mm and with more than 1300 half-period zones is designed with multiple angular levels for integer and fractional topological charges, and the device is fabricated using electron-beam lithography direct writing. The performance of the device is evaluated, and the generation of symmetric and asymmetric donut beams is successfully demonstrated.     

Multiphase Bidirectional Flyback Converter Topology for Hybrid Electric Vehicles

For hybrid electric vehicles, the batteries and the drive dc link may be at different voltages. The batteries are at low voltage to obtain higher volumetric efficiencies, and the dc link is at higher voltage to have higher efficiency on the motor side. Therefore, a power interface between the batteries and the drive's dc link is essential. This power interface should handle power flow from battery to motor, motor to battery, external genset to battery, and grid to battery. This paper proposes a multi-power-port topology which is capable of handling multiple power sources and still maintains simplicity and features like obtaining high gain, wide load variations, lower output-current ripple, and capability of parallel-battery energy due to the modular structure. The scheme incorporates a transformer winding technique which drastically reduces the leakage inductance of the coupled inductor. The development and testing of a bidirectional flyback dc-dc converter for hybrid electric vehicle is described in this paper. Simple hysteresis voltage control is used for dc-link voltage regulation. The experimental results are presented to show the working of the proposed converter.     

Video watermarking using wavelet transform and tensor algebra

We present a robust, hybrid non-blind MPEG video watermarking technique based on a high-order tensor singular value decomposition and the discrete wavelet transform (DWT). The core idea behind our proposed technique is to use the scene change analysis to embed the watermark repeatedly into the singular values of high-order tensors computed form the DWT coefficients of selected frames of each scene. Experimental results on video sequences are presented to illustrate the effectiveness of the proposed approach in terms of perceptual invisibility and robustness against attacks.     

Plasmonic Lipid Bilayer Membranes for Enhanced Detection Sensitivity of Biolabeling Fluorophores

Plasmonics based sensing, using the surface plasmon resonance of metal nanoparticles, has been effectively demonstrated in various applications. Extending this methodology to cell and artificial lipid bilayer membranes is extremely beneficial in enhancing the sensitivity of the detection of binding and cellular transport of molecules across such membranes. Here, the creation of an artificial plasmonic biomembrane template is demonstrated and used to show the enhanced detection sensitivity of certain widely used biomarker molecules. The efficacy of these templates is explained in terms of the ability of the hydrophobic polymer grafted gold nanoparticles used to organize, penetrate, and fluidize the membranes. The enhancement of photoluminescence of the dye molecules used occurs over a reasonably large spectral range as compared to the plasmon resonance of gold nanoparticles. The results could, possibly, be extended to cellular membranes with relevant modifications, as well as to the detection of any other biological molecule appropriately labeled with fluorescent dye molecules, and demonstrate the versatility of these plasmonic bioinspired platforms as potential biochemical sensors.     

Microstructural Characterization of Laser-Deposited Al 4047 Alloy

Direct metal deposition (DMD) technology is a laser-aided rapid prototyping method that can be used to fabricate near net shape components from their CAD files. In the present study, a series of Al-Si samples have been deposited by DMD in order to optimize the laser deposition parameters to produce high quality deposit with minimum porosity and maximum deposition rate. This paper presents the microstructural evolution of the as-deposited Al 4047 sample produced with optimized process parameters. Optical, scanning, and transmission electron microscopes have been employed to characterize the microstructure of the deposit. The electron backscattered diffraction method was used to investigate the grain size distribution, grain boundary misorientation, and texture of the deposits. Metallographic investigation revealed that the microstructural morphology strongly varies with the location of the deposit. The layer boundaries consist of equiaxed Si particles distributed in the Al matrix. However, a systematic transition from columnar Al dendrites to equiaxed dendrites has been observed in each layer. The observed variation of the microstructure was correlated with the thermal history and local cooling rate of the melt pool.