Ice slurry: Pressure drop and deposition velocity

Ice slurries are used in indirect refrigeration systems. The storage tank of ice slurry systems decreases the maximum required cooling capacity of the equipment and smooths down the installation consumption. An advantage of ice slurry systems compared to chilled water systems is the higher cooling energy that can be delivered to customers using the same installed pipes. This paper gives a complete investigation of ice slurry pressure drops and deposition velocity. A model for the friction factor, obtained by empirical and semi-empirical approximation, is discussed. A new simple and efficient method is introduced to find the deposition velocity in an ice slurry fluid flow. Finally a variety of experimental results and some theoretical calculations of ice slurry flow patterns are shown.     

Maternal morbidity and mortality related to delivery

OBJECTIVES: To describe the patterns of growth, nutritional status, body composition, and resting energy expenditure (REE) in prepubertal children with Alagille syndrome (AGS) before the onset of end-stage liver disease. STUDY DESIGN: Thirteen prepubertal subjects with AGS (8 male; mean age, 6.8 2.8 years) were evaluated for growth parameters, body composition by skinfolds and by dual-energy x-ray absorptiometry, and REE by indirect calorimetry. The children with AGS were compared with a healthy, age-matched reference group of 37 prepubertal children. RESULTS: Compared with healthy children, children with AGS had significantly reduced (P <. 05) growth (weight, weight z score, height, height z score), nutritional status (midarm circumference, triceps skinfold, and midarm muscle area), and body composition (fat mass and fat-free mass). Subscapular thickness, percent body fat, and REE were not different. The AGS subgroup (n = 4) with REE greater than 110% predicted value had a reduced percent body fat (P <.02). CONCLUSIONS: Growth and body composition abnormalities are common in prepubertal children with AGS.     

Molecular tools for identification of Penicillium starter cultures used in the food industry.

The main goal of this work was to develop rapid and accurate molecular tools to discriminate species of white industrial Penicillia. We applied three different polymerase chain reaction (PCR) based techniques. Sequences of the ITS region of the rRNA gene unit and of the 5' end of the beta tubulin gene yielded 1.2% and 5.8% nucleotide variability respectively, between Penicillium camembertii and Penicillium nalgiovense. Polymorphic restriction sites were found in both sequences. These may be used in diagnostic PCR-RFLP analysis to rapidly distinguish between the two Penicillium species. Random amplified polymorphic DNA (RAPD) markers were also useful to differentiate these two species, but no polymorphism was found at the subspecific level, which evidenced a high level of homogeneity of the isolates studied. By means of these three techniques, the real identity of industrial strains of Penicillium chrysogenum and P. nalgiovense could be demonstrated. The comparison of these isolates with type strains of the two species suggested that the former corresponds to P. nalgiovense. The genetic relatedness between P. naglovense and Penicillium dipodomyis was also confirmed.     

Unexpected Interacting Effects of Physical (Radiation) and Chemical (Bisphenol A) Treatments on Male Reproductive Functions in Mice

For decades, numerous chemical pollutants have been described to interfere with endogenous hormone metabolism/signaling altering reproductive functions. Among these endocrine disrupting substances, Bisphenol A (BPA), a widely used compound, is known to negatively impact germ and somatic cells in the testis. Physical agents, such as ionizing radiation, were also described to perturb spermatogenesis. Despite the fact that we are constantly exposed to numerous environmental chemical and physical compounds, very few studies explore the impact of combined exposure to chemical and physical pollutants on reproductive health. The aim of this study was to describe the impact of fetal co-exposure to BPA and IR on testicular function in mice. We exposed pregnant mice to 10 µM BPA (corresponding to 0.5 mg/kg/day) in drinking water from 10.5 dpc until birth, and we irradiated mice with 0.2 Gy (γ-ray, RAD) at 12.5 days post-conception. Co-exposure to BPA and γ-ray induces DNA damage in fetal germ cells in an additive manner, leading to a long-lasting decrease in germ cell abundance. We also observed significant alteration of adult steroidogenesis by RAD exposure independently of the BPA exposure. This is illustrated by the downregulation of steroidogenic genes and the decrease of the number of adult Leydig cells. As a consequence, courtship behavior is modified, and male ultrasonic vocalizations associated with courtship decreased. In conclusion, this study provides evidence for the importance of broadening the concept of endocrine disruptors to include physical agents, leading to a reevaluation of risk management and regulatory decisions.     

Mechanisms of Corosion and Oxidation of Metals and Alloys

Selected topics in field of the study of the mechanisms of corrosion and of oxidation of metals or alloys are presented. The first part reports a new model for the mechanism of the breakaway oxidation of ferritic stainless steels in water vapour. The second part is devoted to the physico‐chemical aspects of oxidation and presents experimental methods useful in the kinetic modelling applied to two alloys, the zircalloy‐4 and an AlMg5 % in the liquid state. In the third part the physical and numerical modelling of the stress corrosion cracking behaviour in face‐centered cubic (fcc) alloys is detailed, which enables the study of the influence of macroscopic parameters (such as the temperature or hydrogen activity) on the fracture process.     

The Study of a 231 French Patient Cohort Significantly Extends the Mutational Spectrum of the Two Major Usher Genes MYO7A and USH2A

Usher syndrome is an autosomal recessive disorder characterized by congenital hearing loss combined with retinitis pigmentosa, and in some cases, vestibular areflexia. Three clinical subtypes are distinguished, and MYO7A and USH2A represent the two major causal genes involved in Usher type I, the most severe form, and type II, the most frequent form, respectively. Massively parallel sequencing was performed on a cohort of patients in the context of a molecular diagnosis to confirm clinical suspicion of Usher syndrome. We report here 231 pathogenic MYO7A and USH2A genotypes identified in 73 Usher type I and 158 Usher type II patients. Furthermore, we present the ACMG classification of the variants, which comprise all types. Among them, 68 have not been previously reported in the literature, including 12 missense and 16 splice variants. We also report a new deep intronic variant in USH2A. Despite the important number of molecular studies published on these two genes, we show that during the course of routine genetic diagnosis, undescribed variants continue to be identified at a high rate. This is particularly pertinent in the current era, where therapeutic strategies based on DNA or RNA technologies are being developed.     

Yeast ecology in French cider and black olive natural fermentations

In this study, rDNA ITS restriction analysis was used to identify yeasts from two naturally fermented products: French ciders and black olives. In cider musts and bottled ciders, the PCR-RFLP method generated 15 different ITS/RFLP profiles for a total of 208 isolates. The predominant yeasts corresponded to Saccharomyces bayanus, Saccharomyces cerevisiae, Lachancea cidri and Dekkera anomala. Three identified species: Candida sake, Candida tropicalis and Kluyveromyces marxianus had never been described before in ciders. For the black olive fermentation, the method allowed for identification of 11 profiles for a total of 137 isolates. A sequential apparition of yeasts was observed with Pichia anomala, Candida boidinii and Debaryomyces etchellsii being the predominant species. Four isolates did not correspond to any known species based on the sequencing of the D1/D2 region of the 26S rRNA gene. By using the rDNA ITS method, valuable information on yeast population biodiversity and dynamics in the naturally fermented food products studied was obtained.     

Prion degradation in soil: possible role of microbial enzymes stimulated by the decomposition of buried carcasses

This study is part of a European project focused on understanding the biotic and abiotic mechanisms involved in the retention and dissemination of transmissible spongiform encephalopathies (TSE) infectivity in soil in order to propose practical recommendations to limit environmental contamination. A 1-year field experiment was conducted with lamb carcasses buried in a pasture soil at three depths (25, 45, and 105 cm). Microbial community response to carcasses was monitored through the potential proteolytic activity and substrate induced respiration (SIR). Soil above carcasses and control soil exhibited low proteolytic capacity, whatever the depth of burial. Contrastingly, in soil beneath the carcasses, proteolysis was stimulated. Decomposing carcasses also stimulated SIR, i.e., microbial biomass, suggesting that proteolytic populations specifically developed on lixiviates from animal tissues. Decomposition of soft tissues occurred within 2 months at subsurface while it lasted at least 1 year at deeper depth where proteolytic activities were season-dependent. The ability of soil proteases to degrade the beta form of prion protein was shown in vitro and conditions of burial relevant to minimize the risk of prion protein dissemination are discussed.     

The effect of the temporal structure of spoken words on paired-associate learning.

In a series of experiments, participants learned to associate black-and-white shapes with nonsense spoken labels (e.g., “joop”). When tested on their recognition memory, participants falsely recognized as correct a shape paired with a label that began with the same sounds as the shape’s original label (onset-overlapping lure; e.g., joob) more often than a shape paired with a label that overlapped with the original label at offset (offset-overlapping lure; e.g., choop). Furthermore, the false-alarm rate was modulated by the phonetic distance between the sounds that distinguished the original label and the lures. Greater false-alarm rates to onset-overlapping labels were not predicted by explicit similarity ratings or by consonant identification and were not dependent upon label familiarity. The asymmetry at erroneously recognizing onset- versus offset-overlapping lures remained unchanged as the presentation of the shape at test was delayed in time, suggesting that response anticipation based on the first sounds of the spoken label did not contribute much to the false recognition of onset-overlapping lures. Thus, learning 2 words whose names differ in their last sounds appears to pose greater difficulty than learning 2 words whose names differ in their first sounds because, we argue, people are biased to give more importance to the early sounds of a name than to its last sounds when learning a novel label–referent association. (PsycINFO Database Record (c) 2010 APA, all rights reserved)