Effect of dry fractionated hybrid protein ingredients on the structural,textural, thermal and sensory properties of gluten-free oat and faba pasta

Protein-enriched gluten-free pasta was prepared from oat starch-rich endosperm (SRE), which was substituted with different proportions of oat protein concentrate (OPC) and/or faba protein concentrate (FPC) fractions in order to obtain a similar protein content (18% and 35%). Accordingly, the health claims ‘source of protein’ and ‘high protein’ were achieved. Pasta with OPC and FPC had lower optimal cooking time (8 and 6.3 min), cooking loss (6% and 10.2% dm) and water absorption (152% and 147%) than control SRE pasta (9.3 min; 12.3% dm and 185%). Addition of protein concentrates produced an increase in hardness and chewiness of spaghetti. The glycaemic index of pasta was lowered by the addition of protein ingredients, the lowest value being achieved with FPC. In vitro protein digestibility of pasta increased up to 3.5% for OPC and up to 7.1% in case of FPC addition. The most protein-digestible pasta was that with FPC addition to reach 35% protein content.     

Ovariectomy-Induced Hepatic Lipid and Cytochrome P450 Dysmetabolism Precedes Serum Dyslipidemia

Ovarian hormone deficiency leads to increased body weight, visceral adiposity, fatty liver and disorders associated with menopausal metabolic syndrome. To better understand the underlying mechanisms of these disorders in their early phases of development, we investigated the effect of ovariectomy on lipid and glucose metabolism. Compared to sham-operated controls, ovariectomized Wistar female rats markedly increased whole body and visceral adipose tissue weight (p ˂ 0.05) and exhibited insulin resistance in peripheral tissues. Severe hepatic triglyceride accumulation (p ˂ 0.001) after ovariectomy preceded changes in both serum lipids and glucose intolerance, reflecting alterations in some CYP proteins. Increased CYP2E1 (p ˂ 0.05) and decreased CYP4A (p ˂ 0.001) after ovariectomy reduced fatty acid oxidation and induced hepatic steatosis. Decreased triglyceride metabolism and secretion from the liver contributed to hepatic triglyceride accumulation in response to ovariectomy. In addition, interscapular brown adipose tissue of ovariectomized rats exhibited decreased fatty acid oxidation (p ˂ 0.01), lipogenesis (p ˂ 0.05) and lipolysis (p ˂ 0.05) despite an increase in tissue weight. The results provide evidence that impaired hepatic triglycerides and dysregulation of some CYP450 proteins may have been involved in the development of hepatic steatosis. The low metabolic activity of brown adipose tissue may have contributed to visceral adiposity as well as triglyceride accumulation during the postmenopausal period.     

Environmentally Friendly Cross-Linked Antifouling Coatings Based on Dual Antimicrobial Action

The synthesis of environmentally friendly antimicrobial polymeric coatings, especially in the case of aquaculture, that inhibit the growth of bio-deposits is a very important issue that will contribute to the cost reduction of nets’ cleaning process as well as the protection of the submarine wealth from the biostatic substances used so far. In the present work, the antimicrobial polymers P(SSAmC₁₆-co-VBCHAMx) and the terpolymer P(SSAmC₁₆w-co-VBCHAMx-co-GMAy) were synthesized, bearing quaternary ammonium compounds, electrostatically bound and covalently attached at the same polymer chain. The combination of the two types is of particular importance, as it can provide effective antimicrobial polymeric materials with self-polishing capabilities as a result of the released nature of the antimicrobial, in combination with the permanent local action of the immobilized species. The cross-linking reaction of the terpolymer P(SSAmC₁₆w-co-VBCHAMx-co-GMAy) with the homopolymer polyacrylic acid (PAA) was tested at 120 °C in terms of the equivalent ratio between epoxy and carboxyl groups. The synthesized polymers were further used for the coating of aquaculture nets and tested in terms of antifouling efficiency in lab and scale-up conditions. Uncoated nets were also used in all applications for comparison reasons. The coated nets performed efficiently for 35 days in lab-scale and 66 days in scale-up conditions, showing a high antifouling activity in both fields compared to the uncoated nets.     

Manganese transport through human erythrocyte membranes. An EPR study

Manganese uptake by human erythrocytes was investigated in the concentration range 0.5-20 mM in the suspending solution, by using the EPR technique. S shaped dependencies of manganese influx on manganese doping solution concentration for both fresh and vanadate treated erythrocytes were found, with maximum influx values of 4.1 +/- 1.9 x 10(-10) mol/m2 x s and 2.1 +/- 0.3 x 10(-9) mol/m2 x s, respectively. At low manganese concentrations (< 2 mM) the manganese permeability coefficient increases with increasing the doping concentration, the ions cooperate for achieving a transport event. For high manganese concentration (> 5 mM) the permeability coefficient decreases with increasing the doping concentration, the ions competing for the limited amount of transport system. A similar increase in manganese uptake as in vanadate treated erythrocytes was measured for 'in vitro' aged erythrocytes. These results might suggest that human erythrocytes possess an active transport mechanism by which, they oppose to manganese influx. This hypothesis is also supported by the 10-15 min time lag between the moment of doping and the start of the manganese influx into the fresh erythrocytes. The manganese uptake inhibition by nifedipine, a calcium channel blocker, for the case of vanadate treated erythrocytes, suggests that, at least partially, manganese uptake by the cells occurs via the 'calcium channels'.     

Metformin Affects Cardiac Arachidonic Acid Metabolism and Cardiac Lipid Metabolite Storage in a Prediabetic Rat Model

Metformin can reduce cardiovascular risk independent of glycemic control. The mechanisms behind its non-glycemic benefits, which include decreased energy intake, lower blood pressure and improved lipid and fatty acid metabolism, are not fully understood. In our study, metformin treatment reduced myocardial accumulation of neutral lipids—triglycerides, cholesteryl esters and the lipotoxic intermediates—diacylglycerols and lysophosphatidylcholines in a prediabetic rat model (p < 0.001). We observed an association between decreased gene expression and SCD-1 activity (p < 0.05). In addition, metformin markedly improved phospholipid fatty acid composition in the myocardium, represented by decreased SFA profiles and increased n3-PUFA profiles. Known for its cardioprotective and anti-inflammatory properties, metformin also had positive effects on arachidonic acid metabolism and CYP-derived arachidonic acid metabolites. We also found an association between increased gene expression of the cardiac isoform CYP2c with increased 14,15-EET (p < 0.05) and markedly reduced 20-HETE (p < 0.001) in the myocardium. Based on these results, we conclude that metformin treatment reduces the lipogenic enzyme SCD-1 and the accumulation of the lipotoxic intermediates diacylglycerols and lysophosphatidylcholine. Increased CYP2c gene expression and beneficial effects on CYP-derived arachidonic acid metabolites in the myocardium can also be involved in cardioprotective effect of metformin.     

Earth Observation application development based on the Grid oriented ESIP satellite image processing platform

Satellite images supply important information on earth surface, weather, clime, geographic areas, vegetation, and natural phenomena. Processing of satellite data requires high computation resources and flexible tools in order to search, discover, and reveal the main information, to experiment new algorithms, and to include them into new Earth Observation applications. This paper describes the features and the architectures of the ESIP and gProcess platforms, supporting the Grid based satellite imagery processing. The development methodology of Earth Observation applications is highlighted as well in order to hide the Grid complexity to the user.     

From agency and subjectivity to animism: phenomenological and Science Technology Studies (STS) approach to design of large techno-social systems

The social, ecological and technological challenges, starting on the scale of a small-scale city block to the megapolises and further, force us to rethink the user agency in design. What is needed is a Copernican Revolution that will question the centrality of the user and the human and offer a more balanced model for interaction between different agencies, that is more sensitive to the issues of sustainability, co-dependence and symbiosis which we face within such complex and hybrid systems. One way to go is to rethink the early phenomenological concepts of a subject that is more tightly connected and even defined by its environment (Lifeworld, Dasein). Another approach is to thematise the possibilities and limits of non-human agency and define design in terms of creating new networks and assemblages as described by science technology studies (STS) and posthumanist philosophy.