A 3D Cell-Free Bone Model Shows Collagen Mineralization is Driven and Controlled by the Matrix

Osteons, the main organizational components of human compact bone, are cylindrical structures composed of layers of mineralized collagen fibrils, called lamellae. These lamellae have different orientations, different degrees of organization, and different degrees of mineralization where the intrafibrillar and extrafibrillar minerals are intergrown into one continuous network of oriented crystals. While cellular activity is clearly the source of the organic matrix, recent in vitro studies call into question whether the cells are also involved in matrix mineralization and suggest that this process could be simply driven by the interactions of the mineral with extracellular matrix. Through the remineralization of demineralized bone matrix, the complete multiscale reconstruction of the 3D structure and composition of the osteon without cellular involvement are demonstrated. Then, this cell-free in vitro system is explored as a realistic, functional model for the in situ investigation of matrix-controlled mineralization processes. Combined Raman and electron microscopy indicate that glycosaminoglycans (GAGs) play a more prominent role than generally assumed in the matrix–mineral interactions. The experiments also show that the organization of the collagen is in part a result of its interaction with the developing mineral.     

A preliminary study on the manufacturing aluminum–potassium feldspar metal matrix composite materials

This study reports the results of a preliminary study of manufacturing aluminum–potassium feldspar metal matrix composites including its castability and mechanical properties. The material was composed considering 0.25 %, 0.5 %, and 0.75 % additive ratios to examine the impact of the amount of potassium feldspar on the material and mechanical properties of the manufactured aluminum metal matrix composite material. Aluminum alloy (A356) was utilized as the matrix of the composition. The composite material was manufactured by the stir casting technique. The modulus of elasticity, yield strength, tensile strength, elongation, density, and quality index of the material parameters was taken into account to determine the materials′ practicality. The preliminary results indicated that potassium feldspar is a promising mineral to be utilized as an additive for aluminum metal matrix composites since it has a positive impact on mechanical properties. By optimizing the casting parameters, the positive aspects of the potassium feldspar may make the material more practical to be utilized in vast engineering applications.     

Analytical determination of compounds released during pyrolysis,smoldering, and combustion experiments with insulation materials from renewable resources

Obviously, the use of insulation materials from renewable resources in buildings could provide benefits regarding environmental protection and sustainable management. Nevertheless, their market share in Germany is estimated to be about 7% because of a partial lack of knowledge about their properties, and therefore, of construction certifications. This work was intended to close a knowledge gap concerning emissions during pyrolysis, smoldering, and combustion of commercial insulating materials made from wood, cellulose, meadow grass, hemp, jute, cork, and seaweed as well as polystyrene for comparison reasons. Laboratory-scale experiments were conducted and the measurement of thermal decomposition products was done with gas chromatography combined with mass spectrometry (GC/MS). It was realized that almost all the products could be assigned to the following eight substance classes: carbohydrates, aldehydes/ketones, carbonic acids/esters, substituted phenols, furans, aliphatic hydrocarbons, substituted benzenes, and polycyclic aromatic hydrocarbons. Substance spectra were generated that showed certain conformities, especially between the insulating materials made from wood, cellulose, and meadow grass as well as hemp and jute. Comparisons of the sum of peak areas in the GC/MS-chromatograms provided indications of the relative extent of thermal decomposition product emissions. Calculations according to (∑peak areas _{renewable material})/(∑peak areas _polystyrene) revealed factors between 0.18 (wood, cork) and 0.028 (meadow grass). In the thermal emissions, defined hazardous substances or substance groups were frequently measured. These were included in a toxicity evaluation by which, inter alia, advantages of the natural products compared to polystyrene could be demonstrated.