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A concept for a future integer arithmetic unit suitable for a realization with 3-D optoelectronic very large scale integrated (VLSI) circuits is presented. Due to the use of optical interconnections running vertically to the circuit's surface no pin limitation is given. This allows massively parallelism and a higher throughput performance than in all-electronic solutions. To exploit the potential of optical interconnections in VLSI systems efficiently well-adapted low-level algorithms and architectures have to be developed. This is demonstrated for a pipelined arithmetic unit using a redundant number representation. A transistor layout for the optoelectronic circuits is given as well as a specification for the necessary optical interconnection scheme linking the circuits with free-space optics. It is shown that the throughput can be increased by a factor of 10 to 50 compared to current all-electronic processors by considering state-of-the-art optical and optoelectronic technology. Furthermore we present results we gained by investigations on a first realized optoelectronic VLSI test chip. 相似文献
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Anja Kostelac Leander Sützl Jolanta Puc Valentina Furlanetto Christina Divne Dietmar Haltrich 《International journal of molecular sciences》2022,23(21)
Pyranose oxidase (POx, glucose 2-oxidase; EC 1.1.3.10, pyranose:oxygen 2-oxidoreductase) is an FAD-dependent oxidoreductase and a member of the auxiliary activity (AA) enzymes (subfamily AA3_4) in the CAZy database. Despite the general interest in fungal POxs, only a few bacterial POxs have been studied so far. Here, we report the biochemical characterization of a POx from Streptomyces canus (ScPOx), the sequence of which is positioned in a separate, hitherto unexplored clade of the POx phylogenetic tree. Kinetic analyses revealed that ScPOx uses monosaccharide sugars (such as d-glucose, d-xylose, d-galactose) as its electron-donor substrates, albeit with low catalytic efficiencies. Interestingly, various C- and O-glycosides (such as puerarin) were oxidized by ScPOx as well. Some of these glycosides are characteristic substrates for the recently described FAD-dependent C-glycoside 3-oxidase from Microbacterium trichothecenolyticum. Here, we show that FAD-dependent C-glycoside 3-oxidases and pyranose oxidases are enzymes belonging to the same sequence space. 相似文献
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Melanie Holzwarth Sabine Korhummel Reinhold Carle Dietmar R. Kammerer 《European Food Research and Technology》2012,234(2):207-222
Strawberry purées were prepared using a commercial polygalacturonase (PG) and a highly purified pectinesterase (PE) preparation,
respectively. To elucidate the effect of pectin on color stability following enzymatic pulp maceration, pectin composition
was studied by isolating and fractionating the alcohol-insoluble residue from the strawberry purées. The purées were stored
at +20 and +4 °C in the dark over a period of 24 weeks monitoring the amounts of monomeric and polymeric anthocyanins as well
as antioxidant activities (FRAP, TEAC). Individual anthocyanins were analyzed by HPLC–DAD–MS
n
, and color measurements were obtained in the CIE L*a*b* system. Pectin composition was significantly modified following enzymatic maceration of the purées. While PG treatment generally
resulted in pectin losses, oxalate-soluble pectins were increased in PE-treated purées. After 24 weeks of storage, the best
anthocyanin retention was observed in PE-treated purées. Such products also revealed greatest anthocyanin half-life values
and lowest proportion of polymeric pigments. Compared to an untreated control, enzymatic purée maceration using the PG was
also beneficial for pigment retention, but less effective than PE. In contrast, color and antioxidant activity were independent
of both enzymatic treatments. An initial heating step (90 °C, 10 s) for immediate inactivation of native enzymes such as polyphenoloxidases
slightly improved pigment stability, while lowered temperature during mash maceration was less effective. However, by far
best color and pigment retention were achieved when the purées were stored at 4 °C in the dark. 相似文献
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Spreadsheet programs are probably the most successful example of end-user software development tools and are used for a variety of purposes. Like any type of software, they are prone to error, in particular as they are usually developed by non-programmers. While various techniques exist to support the developer in finding errors in procedural programs, the tool support for spreadsheet debugging is still limited. In this paper, we show how techniques from model-based diagnosis can be applied and extended for spreadsheet debugging by translating the relevant parts of a spreadsheet to a constraint satisfaction problem. We additionally propose both problem-specific and generalizable extensions to the classical diagnosis algorithms which help to detect potential problems in a spreadsheet based on user-provided test cases more efficiently. The proposed techniques were integrated into a modular framework for spreadsheet debugging and evaluated with respect to scalability based on a number of real-world and artificially created spreadsheets. An additional error detection exercise involving 24 subjects was performed to assess the general applicability of such advanced spreadsheet debugging techniques for end users. 相似文献