Anticancer Potential of (Pentamethylcyclopentadienyl)chloridoiridium(III) Complexes Bearing κP and κP,κS‐Coordinated Ph2PCH2CH2CH2S(O)xPh (x=0–2) Ligands

Iridium(III) complexes of the type [Ir(η⁵‐C₅Me₅)Cl₂{Ph₂PCH₂CH₂CH₂S(O)_xPh‐κP}] (x=0–2; 1 – 3 ) and [Ir(η⁵‐C₅Me₅)Cl{Ph₂PCH₂CH₂CH₂S(O)_xPh‐κP,κS}][PF₆] (x=0–1; 4 and 5 ) with 3‐(diphenylphosphino)propyl phenyl sulfide, sulfoxide, and sulfone ligands Ph₂PCH₂CH₂CH₂S(O)_xPh were designed, synthesized, and characterized fully, including X‐ray diffraction analyses for complexes 3 and 4 . In vitro studies against human thyroid carcinoma (8505C), submandibular carcinoma (A253), breast adenocarcinoma (MCF‐7), colon adenocarcinoma (SW480), and melanoma (518A2) cell lines provided evidence for the high biological potential of the neutral and cationic iridium(III) complexes. Neutral iridium(III) complex 5 proved to be the most active, with IC₅₀ values up to about 0.1 μM , representing activities of up to one order of magnitude higher than cisplatin. Using 8505C cells, apoptosis was shown to be the main mechanism through which complex 5 exerts its tumoricidal action. The described iridium(III) complexes represent potential leads in the search for novel metal‐based anticancer agents.     

Fast and accurate NURBS fitting for reverse engineering

Fast and accurate fitting of non-uniform rational B-spline (NURBS) curves and surfaces through large sets of measured data is an important problem in applications such as reverse engineering and geometric modelling. This paper presents a method for realising significant improvements in the computational efficiency of this task. The basic idea is that the sparsity structures of the relevant matrices that are specific to the problem of NURBS fitting can be precisely defined and that full exploitation of these structures leads to significant savings in both computational and storage requirements. These savings allow for a large number of control points to be used in order to define the surface and consequently to improve the accuracy of shape representation. The achieved computational complexity is linear in both the number of measured points and the number of control points while the storage requirements of the algorithm are linear with the number of control points only. The complexity analysis, as well as the analysis of actual running times is presented. The results demonstrate that, using this approach, highly complex shapes may be modelled accurately with a single NURBS surface.     

热力学法在泵性能测试中的应用及与传统方法的比较

全球范围而言,水泵效率和性能的热力学测试方法大约于十多年前在工业领域开始得到大量应用,但目前在泵站测试中使用还不是很广泛。由于缺少直接的工业应用标准去保证热力学法测试结果的准确可靠,且多数泵站自身又缺乏足够经验来开展独立研究测试,所以迫切需要一些通用性的指导原则来推动相关领域的进展。通过对多城市多泵站的水泵效率测试和研究,本项目完成了41个泵站的152个城市供水水泵的性能测试。其中57个水泵同时使用了热力学法和传统方法。通过大量的现场测试,并与采用电磁流量计、超声波流量计等传统测试方法的对比,积累了许多关于热力学法和传统方法在水泵性能测试中的经验和技巧;测试研究成果进一步明确了热力学法的适用条件和范围,通过对实测中遇到的各种情况的处理和总结,提出了热力学方法的通用性指导应用原则。     

Evaluation of antimicrobial and proteolytic activity of enterococci isolated from fermented products

A collection of 26 enterococci isolated from dairy and meat products were tested for antimicrobial and proteolytic activity. Enterococcus faecium and E. faecalis were the most frequent species among tested enterococci, and 11 isolates produced antimicrobial compounds. Results revealed that 10 out of 11 enterococci synthesized enterocins showing antimicrobial activity against food-born pathogen such as Listeria monocytogenes and Staphylococcus aureus. The broadest spectrum of antimicrobial activity was detected in E. faecalis BGPT1-10P and BGPT1-78. E. faecalis BG221 showed antimicrobial activity that was not related to production of enterocin, H₂O₂ or organic acid. Twenty-five enterococci showed strong or moderate proteolytic activity towards β-casein. Two isolates, BGPT1-10P and BGPT1-78, showed the most intense hydrolysis of α_s1-, β-, κ-casein fractions, total casein as well as gelatin. Extracellular BGPT1-10P and BGPT1-78 proteinases have a molecular mass of about 29 kDa. Bacteriocin production and proteinase activity of natural isolates of enterococci may be of technological interest in dairy and meat-fermented products.     

New method for assessing the performance of agricultural biogas plants

Around 5000 agricultural biogas plants were operating in Germany by the end of 2009. There is a recognized need for their assessment, comparison and performance improvement. Prerequisites for their reliable assessment include: 1) detailed and reliable performance data, 2) defined criteria for the assessment, and 3) comprehensive method. The limitations of existing assessment methods have been reviewed. The new method developed for assessing the overall performance of biogas plants is focused on four assessment aspects: biogas production, biogas utilization, environmental impact and socio-economic efficiency. Each assessment aspect was determined by two performance criteria. The method is based on fuzzy set theory and fuzzy mathematics, which enables use of imprecise and uncertain data. Incorporation of enough expertise from the field of biogas technology was enabled in this method as well. Pre-assessment of future biogas plants in their planning phase is possible, contributing to their effective development. The reliability of the method was tested using data from ten monitored biogas plants. The assessment of the ten biogas plants showed that biogas utilization is the aspect with the largest potential for performance improvement, by increasing the external heat utilization. The method developed is adjustable to accommodate new developments related to biogas technology and biogas plants in any geographical region. Future research should be focused on definition and inclusion of more assessment criteria and on developing the methods that are capable to handle compensation between individual criteria.     

A CAD/CAE-integrated injection mold design system for plastic products

Mold design is a knowledge-intensive process. This paper describes a knowledge-based oriented, parametric, modular and feature-based integrated computer-aided design/computer-aided engineering (CAD/CAE) system for mold design. Development of CAx systems for numerical simulation of plastic injection molding and mold design has opened new possibilities of product analysis during the mold design. The proposed system integrates Pro/ENGINEER system with the specially developed module for the calculation of injection molding parameters, mold design, and selection of mold elements. The system interface uses parametric and CAD/CAE feature-based database to streamline the process of design, editing, and reviewing. Also presented are general structure and part of output results from the proposed CAD/CAE-integrated injection mold design system.     

The effect of lignin on the structure and characteristics of composite coatings electrodeposited on titanium

Composite coatings based on lignin, obtained by electrophoretic deposition (EPD) on titanium, were investigated. The aim of this work was to produce hydroxyapatite/lignin (HAP/Lig) coatings on titanium and to investigate the effect of the lignin concentration on microstructure, morphology, phase composition, thermal behavior and cytotoxicity of the HAP/Lig coatings. An organosolv lignin was used for the production of the composite coatings studied in this research. The properties of HAP/Lig coatings were characterized using X-ray diffraction (XRD) analysis, scanning electron microscopy (SEM), thermogravimetric analysis (TGA), attenuated total reflection Fourier transform infrared spectroscopy (ATR-FT-IR) and X-ray photoelectron spectroscopy (XPS), as well as the MTT test of cytotoxicity. The results showed that higher lignin concentrations protected the HAP lattice during sintering, thereby improving the stability of the HAP/Lig coatings. The cell survival of peripheral blood mononuclear cells (PBMC) after proliferation indicates that the HAP/Lig coating with 1 wt% Lig electrodeposited on titanium was non-toxic with significant promise as a potential bone-repair material.     

The Impact of Gut Microbiota-Derived Metabolites in Autism Spectrum Disorders

Autism Spectrum Disorder (ASD) is a set of neurodevelopmental disorders characterised by behavioural impairment and deficiencies in social interaction and communication. A recent study estimated that 1 in 89 children have developed some form of ASD in European countries. Moreover, there is no specific treatment and since ASD is not a single clinical entity, the identification of molecular biomarkers for diagnosis remains challenging. Besides behavioural deficiencies, individuals with ASD often develop comorbid medical conditions including intestinal problems, which may reflect aberrations in the bidirectional communication between the brain and the gut. The impact of faecal microbial composition in brain development and behavioural functions has been repeatedly linked to ASD, as well as changes in the metabolic profile of individuals affected by ASD. Since metabolism is one of the major drivers of microbiome–host interactions, this review aims to report emerging literature showing shifts in gut microbiota metabolic function in ASD. Additionally, we discuss how these changes may be involved in and/or perpetuate ASD pathology. These valuable insights can help us to better comprehend ASD pathogenesis and may provide relevant biomarkers for improving diagnosis and identifying new therapeutic targets.     

Properties of thermomechanically rolled Nb/Ti microalloyed steel sheets

Structure and mechanical properties of a Nb/Ti microalloyed steel, thermomechanically rolled on a strip-production facility, are evaluated. Small (0.02%) titanium addition to niobium-containing steel is found to increase precipitation while reducing grain-refining potential of niobium. This effect increases with increasing finish-rolling temperature, and is also reflected in an increased charpy energy transition temperature. A reduced tendency for precipitation in a nitrogen-free austenite together with the short interpass times in the finishing train, is assumed to reduce the tendency for pancaking, which is prerequisite for an effective grain refining.