MALDI matrices for biomolecular analysis based on functionalized carbon nanomaterials

When used in small molar ratios of matrix to analyte, derivatized fullerenes and single wall nanotubes are shown to be efficient matrices for matrix-assisted laser desorption/ionization (MALDI) mass spectrometry. The mixing of an acidic functionalized fullerene with a solution of bioanalyte, depositing a dried droplet, and irradiating with a pulsed nitrogen laser yields protonated or cationized molecular ions. Derivatized fullerenes could offer several advantages over conventional MALDI matrices: a high analyte ionization efficiency, a small molar ratios (less than 1) of matrix/analyte, and a broader optical absorption spectrum, which should obviate specific wavelength lasers for MALDI acquisitions. The major disadvantage to the use of fullerenes is the isobaric interference between matrix and analyte ions; however, it is overcome by using MALDI-ion mobility time-of-flight (IM-oTOF) mass spectrometry to preseparate carbon cluster ions from bioanalyte ions prior to TOF mass analysis. However, an alternative to the dried droplet preparation of fullerene MALDI samples is the aerosolization of matrix-analyte solutions (or slurries) followed by impacting the aerosol onto a stainless surface. We also demonstrate that the fullerene matrices can be used to acquire spectra from rat brain tissue.     

Isotopic fractionation during diffusion of transition metal ions in solution

Isotope ratios and elemental concentrations were measured in aqueous solutions sampled at varying distances from sources of Fe or Zn ions. The measurements reveal fractionation of isotopes resulting from pure diffusion in solution. Our data demonstrate that diffusion alone can cause changes in (56)Fe/(54)Fe and (66)Zn/(64)Zn isotope ratios in excess of -0.3 per thousand. These findings thus confirm previous suspicions that transport processes contribute to observed variations in isotopic compositions. Diffusion must therefore be considered when attempting to make inferences from isotope measurements on samples originating from aqueous systems where concentration gradients may develop.     

Apertureless tip-enhanced Raman microscopy with confocal epi-illumination/collection optics

It is demonstrated that confocal epi-illumination/collection optics can be effectively used to generate surface-enhanced Raman scattering at the near-field region of a gold-coated tip for an atomic force microscope operated in semi-contact tapping mode. When the tip, with a 50-nm apex radius, was illuminated by a highly focused laser beam at 532 nm and approached the isolated diamond particle, with a size of approximately 1 microm, the Raman signal was enhanced by approximately 10(3). This result is in good agreement with numerical simulations performed by the finite difference time-domain method. Since our apertureless microscope is based on readily available conventional components, there is wide room for improvements and modifications by common users in various applications of micro-Raman analysis.     

Effect of stream swirling on the velocity and temperature distributions in a round tube

The results of a numerical study of the effect of stream swirling on the laminar flow structure and heat exchange in a round tube are presented.     

Transformations of fuzzy graphs specified by <Emphasis Type="Italic">FD</Emphasis>-grammars

The categorical approach is proposed to formalize transformations of FD-graphs that consist of networks of distributed components whose nodes are specified by fuzzy graphs. Necessary and sufficient conditions are formally defined for FD-graph transformations that do not violate structure integrity and can be constructed componentwise. FD-grammars that generalize fuzzy graph grammars are proposed to describe the admissible transformations of FD-graphs. __________ Translated from Kibernetika i Sistemnyi Analiz, No. 2, pp. 129–147, March–April 2007.     

A mass spectrometer with a membrane interface for oil concentration monitoring in seawater

An immersion mass spectrometer with a membrane interface was used for oil detection and oil concentration measurements in seawater by measuring in situ the concentrations of three hydrocarbons: benzene, toluene, and xylene in the region of the specialized Primorsk oil loading seaport in the Gulf of Finland. The recorded mass spectra demonstrated the possibility of measuring the oil concentration in seawater and determining the grade of oil products. The use of a mass spectrometer with a membrane separator interface allows measurements of hydrocarbon concentration with high accuracy, which is currently not provided in commercially available monitors.     

LMI-based H ∞-optimal control with transients

In this article, the worst-case norm of the regulated output over all exogenous signals and initial states as a performance measure of the system is characterised in terms of linear matrix inequalities (LMIs). Optimal time-invariant state- and output-feedback controllers are synthesised as minimising this performance measure. The essential role in this synthesis plays a weighting matrix reflecting the relative importance of the uncertainty in the initial state contrary to the uncertainty in the exogenous signal. H _∞-optimal control with transients is shown to be actually a trade-off between H _∞-control, being optimal under unknown exogenous disturbances and zero initial state, and γ-control, being optimal under zero exogenous signal and unknown initial conditions, if and only if the weighting matrix satisfies a fundamental inequality. If this inequality is met, the performance measure is achieved and the explicit formulae for the worst-case disturbance and initial state are provided. If this inequality fails, the performance measure coincides with the H _∞-norm and the trade-off gets broken.     

Survey on hierarchical routing schemes in?��flat�� distributed hash tables

The first generation of DHT designs offered a completely flat structure of the key space that is randomly partitioned among participating DHT nodes. That has certain advantages, for example, even distribution of workload among nodes. On the other hand, grouping keys under a single authority or achieving latency guarantees for queries is difficult. To address these shortcomings, various kinds of hierarchy have been proposed over recent years. The last generation is hierarchical DHTs (HDHTs) where nodes are organized onto layers; each next layer consists of supernodes for the previous layer. In this survey paper, we thoroughly go over the evolution of DHTs from pure flat to pre-hierarchical. Our focus is on hierarchical schemes in DHT routing. We argue that their application is not restricted within HDHT designs. We sequentially built a set of design principles; each provides a base for hierarchical routing schemes. In the extreme case, application these principles leads to HDHT designs.