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981.
Shikimate dehydrogenase (E.C.1.1.1.25), extracted from heart-of-palm, was purified by precipitation with ammonium sulphate and elution from columns of Sephadex G-25, and DEAE-Sephadex A-50. The enzyme was unstable and it was not possible to maintain the activity by the addition of cysteine and 2-mercaptoethanol to the buffer. The heart-of-palm contained two isoenzymes which were separated by polyacrylamide gel electrophoresis. The enzyme was inhibited by metal ions and p-CMB. The p-CMB inhibition was reversed by cysteine. The shikimate dehydrogenase was competitively inhibited by protocatechuic acid (Ki 7.5 × 10?4M), and Km values of 0.021 mM and 0.011 mM for shikimic acid and NADP+, respectively, were obtained at pH 9.5 in Tris-HCl-glycine buffer. The molecular weight determination was performed on Sephadex G-100 and the MW was estimated to be 50,000 daltons. 相似文献
982.
Vasilenko O. A. Maier A. A. Chashchin V. A. Gulaeva E. N. Bus'ko V. I. 《Journal of Engineering Physics and Thermophysics》1985,49(3):1105-1108
A method of calculating the temperature resistance coefficient and its temperature dependence for resistive materials in the approximation of a matrix-statistical structure is outlined.Translated from Inzhenerno-Fizicheskii Zhurnal, Vol. 49, No. 3, pp. 477–481, September, 1985. 相似文献
983.
E. WITTIG DE PENNA P. CARREñO X. URRUTIA L. LOPEZ D. BALLESTER 《Journal of food science》1987,52(5):1434-1435
Cookies enriched with 0, 5, 10, 15, 20, and 25% full-fat sweet lupine flour (FFSL) were evaluated by a sensory panel using the rank of preference and paired comparison tests. Cookies with 0, 5, and 10% FFSL were preferred while those containing 20 and 25% FFSL were rejected (p≤0.01). Studies conducted with school children showed similar acceptability for 0 and 10% FFSL-containing cookies which was different (p = 0.05) from those containing 20% FFSL. Fortification of the basic formula with 10% FFSL was recommended on the basis of acceptability. 相似文献
984.
E. Leflaive 《Geotextiles and Geomembranes》1985,2(1):23-30
Over the last decade there has been much interest shown in the use of textiles in civil engineering construction, particularly in geotechnical engineering where geotextiles are associated with soil. Is this a fad, or something more profound and durable? In attempting to rationalize this dramatic development it is concluded that geotextiles do offer many technical and economic advantages. However, at present geotextiles are commonly used as a replacement for traditional building materials using traditional construction methods rather than more innovative techniques that could only be employed using geotextiles. It is concluded that the development of these techniques is the real future of geotextiles and that this future can only be secured by the close cooperation of both textile and civil engineers. 相似文献
985.
E. Kh. Shakhpazov I. G. Rodionova O. N. Baklanova A. I. Zaitsev D. A. Burko A. D. Gorin S. D. Zinchenko 《Metallurgist》2006,50(1-2):65-70
It is shown that the corrosion resistance of automobile sheet can be improved by ensuring that steels of this type have a
low content of corrosion-active nonmetallic inclusions (CANI). The main mechanisms responsible for the formation of CANI in
automobile sheet have been established, and recommendations have been developed to reduce their concentration in this product
• optimize the deoxidation processes that are used by ensuring that the proportions of the additions made to the steel are
consistent with the composition of the ladle slag and that certain key temperature-time parameters of these processes are
maintained
• control the composition of the ladle slag to prevent the formation of solid phases in the slag and their transfer to the
metal; certain conditions must also be observed in the argon blow and the introduction of the addtions.
Use of the above recommendations to make automobile sheet and tube steels cleaner with respect to their content of CANI and
thus help optimize out-of-furnace treatments at metallurgical and pipe plants will make it possible to improve the corrosion
resistance of metal products used in automotive manufacturing.
__________
Translated from Metallurg, No. 2, pp. 45–48, February, 2006. 相似文献
986.
The San Diego County Water Authority of California has initiated planning for coastal desalination facilities to augment their water supplies. Integration of the different water qualities from these facilities into existing pipelines must be achieved. This investigation determined whether, and to what degree, consumers can discriminate between desalinated seawater and imported water supplies and how these investigations can contribute to decision making regarding the need for construction of facilities to blend such supplies prior to delivery. Based upon the results of the flavour profile analysis panel and the consumer evaluation sessions, it was concluded that free chlorine versus chloramine disinfection or different concentrations of disinfectants did not significantly affect consumer perception of the taste and odour of desalinated seawater or blends with Colorado River water and State project water. Consumers were able to discern between desalinated seawater and imported water, preferring imported water when forced to make a choice. However, the investigators did not believe that the difference in consumer perception was significant enough to warrant special blending facilities to mitigate the relatively minor aesthetic quality differences between imported water supplies and desalinated seawater. 相似文献
987.
988.
Operation of a 16-tap fiber-optic delay line configured as a bandpass filter with a fundamental frequency of 2.0 GHz over the frequency range from 0 to 12.5 GHz is discussed. Equal tap weights and spacings were used to facilitate comparison of experimental and predicted results. More than 1011 analog multiplications per second are performed in this relatively simple signal processing system. Other processors for performing functions such as matched filtering and correlation can be implemented by appropriate choice of the mirror reflectances and fiber lengths 相似文献
989.
990.
Beta-lactamase-producing mutants of Streptococcus cremoris 总被引:1,自引:0,他引:1
Penicillin-resistant mutants were isolated for six strains of Streptococcus cremoris used in commercial Cheddar cheese manufacture after treatment with N-methyl-N-nitro-N-nitrosoguanidine. The resistant mutants had an elevated minimal growth inhibitory concentration, 2.5 micrograms (4.13 units)/ml, for penicillin G and other beta-lactam antibiotics as compared with the penicillin-susceptible parent strains, which were each sensitive to .05 micrograms (.08 units)/ml. Penicillin resistance was due to the production of beta-lactamase. Plasmid DNA was not demonstrated in partially purified lysates of four mutants. Mutants had normal cellular morphology but altered phage sensitivity patterns. All except one strain were able to support complete phage adsorption. Resistance was retained after 20 passages in absence of penicillin. 相似文献