Histochemical characterization of the sialic acid residues in mouse colon mucins

The mucins of colonic murine mucus are highly O‐glycosilated sulfosialoglycoproteins. We have characterized the sialylation pattern of oligosaccharide chains of colonic murine mucins by conventional histochemical methods and by lectin histochemistry combined with chemical pretreatments and sialidase digestion. Oligosaccharide chains are strongly sulphated, with an increase of sulfation from the proximal toward the distal colon and a decrease of sialic acid expression and acetylation toward the distal colon. In the goblet cells of proximal colon, sialic acid bound α2,3 to Galβ1,3GalNAc subterminal dimers is diacetylated at C7,C8;C7,C9;C8,C9 or triacetylated at C7,8,9. In the distal colon, sialic acid‐linked α2,3 to Galβ1,3GalNAc subterminal dimers shows reduced O‐acetylation at C7 and/or C8, while acetyl substituents at C9 and at C4 are almost absent. Sialic acid is involved in different essential physiological functions; thus, alterations of its expression and acetylation in oligosaccharide chains of intestinal mucins are generally associated with diseases, such as ulcerative colitis and cancer. Mice may represent a suitable animal model to study alterations of oligosaccharidic chains in colonic mucins and lectin histochemistry combined with chemical pretreatments, and enzyme digestion may be a valuable tool for this study. Our present work may represent a landmark for further lectin histochemical studies to evaluate alterations of mouse colon mucins under different physiological, pathological, or experimental conditions, with possible translational value in humans. Microsc. Res. Tech., 2013. © 2012 Wiley Periodicals, Inc.     

Finite memory non-linear model of a S/H-ADC device

A new model has been proposed for the characterisation of the non-linear dynamic effects of a S/H-ADC device and a new mathematical approach has been introduced to describe its non-ideal dynamic behaviour. The measurement procedure to deduce the parameters of the proposed model is given together with the experimental results on a commercial device.     

A Framework for Using Web Services to Enhance QoS for Content Delivery

An integrated approach to quality of service for content delivery using Web services includes quality definitions, user contracts, and fault monitoring.     

Genotyping of fig (Ficus carica L) via RAPD markers

The genetic diversity among 15 fig accessions (Ficus carica L), belonging to 9 renowned cultivars of the Calabrian fig collection, and 24 unidentified genotypes of figs also located in the Calabrian region of Southern Italy, was investigated by random amplified polymorphic DNA (RAPD) analysis. The genetic similarity values were calculated for the 39 samples, and a dendrogram was elaborated by cluster analysis according to the UPGMA algorithm. The generated DNA fragments grouped the samples into two main clusters of RAPD profiles. Most of the 24 unknown samples, coming from the Luzzi area, formed a unique cluster with high degree of genetic similarity. This indicates that it is possible to distinguish, at DNA level, the fig trees with an already well‐known potential to produce figs suitable for the drying process and possibly to specify cultivars with suitable features for industrial transformation. Copyright © 2005 Society of Chemical Industry     

Radioactive Marker Measurements in Heterogeneous Reservoirs: Numerical Study

A numerical study is performed to simulate the vertical deformation of a depth interval representing a marker spacing (10.5 m) located in a deep heterogeneous sedimentary reservoir. Realistic lithostratigraphic sequences typical of the Northern Adriatic basin are used. A number of scenarios are addressed consistent with the available data. In particular two basic geologic scenarios at the marker scale are simulated, one where sands prevail within the marker spacing (SD), and another where thin sandy and clayey layers alternate (CL). The sensitivity of the marker response is investigated in relation to clay and sand permeability and compressibility contrast, Biot’s coefficient, and respective position of monitoring and fluid pumping wells. The modeling results show that rock may indeed expand above and below depleted layers. Depending on the marker position the expansion may partially offset the compaction, especially in the CL scenario with a very low permeable clay. To obtain a representative field compaction the markers should span a depth interval made mostly by sand and entirely depleted, and should be installed in a test hole far from producing wells. Compressibility contrast and the Biot coefficient play a secondary role. Very critical measurements are provided by two markers which incorporate a thin (≈1 m) depleted level overlain and underlain by almost impermeable clay layers.     

An agent‐based approach for managing e‐commerce activities

In this article, we propose an agent‐based approach for managing e‐commerce activities. In our approach, an agent is present in each e‐commerce site, managing the information stored there. In addition, another agent is associated with each customer, handling his/her profile. The proposed approach is based on the use of a particular conceptual model called the Behaviour‐Semantic Distance and Relevance (B‐SDR) network, which is capable of uniformly representing and handling information stored in e‐commerce sites and customer profiles. The capabilities of the B‐SDR network model are exploited to let customer and site agents cooperate in such a way in order to support a customer in identifying, whenever he/she accesses an e‐commerce site, those products and services present in the site itself and for better matching his/her interests. The approach has been implemented in a prototype in which its functionalities are discussed here also. © 2004 Wiley Periodicals, Inc.     

An improved algorithm for Boolean matrix multiplication

A new algorithm for computing the product of two arbitraryN×N Boolean matrices is presented. The algorithm requiresO (N ³/logN) bit operations and onlyO(N logN) bits of additional storage. This represents an improvement on the Four Russians' method which requires the same number of operations but usesO(N ³/logN) bits of additional storage.     

Star-coupler-based optical cross-connect switch experiments withtunable receivers

An optical cross-connect switch using the star-coupler-based frequency-division-multiplex technique are discussed. Two specific tunable receivers have been implanted. The first is a heterodyne receiver with a tunable laser as the local oscillator (LO) and the second is a tunable filter followed by a direct-detection receiver. In the heterodyne receiver, the tunable LO was a monolithic three-section multiple-quantum-well distributed Bragg laser capable of a 1000-GHz tuning range. Receiver sensitivity was measured to be -38 dBm at 1 Gb/s (BER=10^-10). The power margin in the system substantiated feasibility for a 400×400 switch. In the tunable-filter receiver, the tunable filter is a tunable two-stage optical fiber Fabry-Perot filter design consisting of a narrowband filter followed by a wideband filter. The tuning of the filters is computer controlled, and the combined filter has a tuning range of 15000 GHz with a finesse of ≈5170. Therefore, it is capable of covering over 1000 channels of 2.9 GHz each