Matrilin-3-Primed Adipose-Derived Mesenchymal Stromal Cell Spheroids Prevent Mesenchymal Stromal-Cell-Derived Chondrocyte Hypertrophy

Adipose-derived mesenchymal stromal cells (Ad-MSCs) are a promising tool for articular cartilage repair and regeneration. However, the terminal hypertrophic differentiation of Ad-MSC-derived cartilage is a critical barrier during hyaline cartilage regeneration. In this study, we investigated the role of matrilin-3 in preventing Ad-MSC-derived chondrocyte hypertrophy in vitro and in an osteoarthritis (OA) destabilization of the medial meniscus (DMM) model. Methacrylated hyaluron (MAHA) (1%) was used to encapsulate and make scaffolds containing Ad-MSCs and matrilin-3. Subsequently, the encapsulated cells in the scaffolds were differentiated in chondrogenic medium (TGF-β, 1–14 days) and thyroid hormone hypertrophic medium (T3, 15–28 days). The presence of matrilin-3 with Ad-MSCs in the MAHA scaffold significantly increased the chondrogenic marker and decreased the hypertrophy marker mRNA and protein expression. Furthermore, matrilin-3 significantly modified the expression of TGF-β2, BMP-2, and BMP-4. Next, we prepared the OA model and transplanted Ad-MSCs primed with matrilin-3, either as a single-cell suspension or in spheroid form. Safranin-O staining and the OA score suggested that the regenerated cartilage morphology in the matrilin-3-primed Ad-MSC spheroids was similar to the positive control. Furthermore, matrilin-3-primed Ad-MSC spheroids prevented subchondral bone sclerosis in the mouse model. Here, we show that matrilin-3 plays a major role in modulating Ad-MSCs’ therapeutic effect on cartilage regeneration and hypertrophy suppression.     

Dewatering and Drying Methods for Microalgae

Microalgae can efficiently fix carbon dioxide through their phototropic metabolism, and have been recognized as a promising bioresource for animal feed, health food, fuel, cosmetic, and pharmaceutical products. However, since microalgae in cultivated medium have a low biomass concentration (0.1–1% w/w), both harvesting and concentration of microalgal biomass are often required prior to the production of commercial products. Efficient and cost-effective dewatering and drying methods for microalgae heavily affect the overall energy consumption and production cost of microalgal products. This review describes the characteristics of commonly used dewatering and drying technologies, and critically evaluates the feasibility for their use to treat microalgal biomass. No single dewatering or drying method can satisfactorily handle all types of microalgae. The suitability of each method depends on the properties of the microalgae suspension, the required process design, the quality of the end product, and the related capital and production costs.     

Development of methods for determining free fatty acid contents in red colored oils

Food Science and Biotechnology - Buah merah oil and red palm oil are red colored and unrefined edible oils. Because of this color characteristic, measuring acid value by titration method can be...     

Properties and applications of β‐glycosidase from Bacteroides thetaiotaomicron that specifically hydrolyses isoflavone glycosides

To modify the glycan part of glycosides, the gene encoding β‐glycosidase was cloned from Bacteroides thetaiotaomicron VPI‐5482. The cloned gene, bt_1780, was expressed in Escherichia coli MC1061 and the expressed enzyme was purified using Ni‐NTA affinity chromatography. The purified enzyme, BTBG, showed optimal activity at 50 °C and pH 5.5. Interestingly, this enzyme did not have any hydrolysing activity on ordinary β‐linkage–containing substrates such as xylobiose, lactose and cello‐oligosaccharide, but specifically hydrolysed isoflavone glycosides such as daidzin, genistin and glycitin. Compared to a commercial beta glucosidase, BTBG selectively hydrolysed isoflavone glycosides in soybean extract mixture solution. These results suggest that BTBG may be a specialized enzyme for the hydrolysis of glycosides and that the substrate specificity of BTBG is applicable for the bioconversion of isoflavone glycosides in the food industry.     

Transparent niobate glass-ceramics for optical limiting

The construction of nonlinear optical materials featuring asymmetric transmission of light is of great technological importance for various applications, including optical switching and optical power limiting. A significant challenge is the scalable fabrication of material candidates with good photochemical stability, high optical transmittance, and excellent optical limiting performance. Here, we present a nanocrystallization avenue for constructing hybrid optical limiting materials that exhibit ultrafast and robust optical limiting performance. The experimental results show that the controllable relaxation of a niobate glass may lead to the clustering of Nb-O units and contracting of the bandgap. It results in the notable improvement in nonlinear optical properties, including the enhanced saturation irradiance (380 GW/cm²), doubly increased nonlinear coefficient, and decreased limiting threshold (200 GW/cm²). Our results suggest a promising material that exhibits promising applications for protecting eyes and sensitive components from laser-induced damage.     

简易棋盘格图像标定系统应用

棋盘格图像在摄像机标定中有非常广泛的应用,对于复杂环境且测量精度要求非常高的场合,会需要特定的且有一定复杂度的算法,但通常情况下,使用这样的标定系统是没有必要的,且会让初次应用的人觉得困难。这里提供一套应用简单,快速,对棋盘格图像具有旋转不变性,良好的鲁棒性,且对非复杂环境具有高精度的标定系统。所有算法均是在前辈们的算法上稍作改变而来,应用效果非常好。