Application potential of bone marrow mesenchymal stem cell (BMSCs) based tissue-engineering for spinal cord defect repair in rat fetuses with spina bifida aperta

Spina bifida aperta are complex congenital malformations resulting from failure of fusion in the spinal neural tube during embryogenesis. Despite surgical repair of the defect, most patients who survive with spina bifida aperta have a multiple system handicap due to neuron deficiency of the defective spinal cord. Tissue engineering has emerged as a novel treatment for replacement of lost tissue. This study evaluated the prenatal surgical approach of transplanting a chitosan–gelatin scaffold seeded with bone marrow mesenchymal stem cells (BMSCs) in the healing the defective spinal cord of rat fetuses with retinoic acid induced spina bifida aperta. Scaffold characterisation revealed the porous structure, organic and amorphous content. This biomaterial promoted the adhesion, spreading and in vitro viability of the BMSCs. After transplantation of the scaffold combined with BMSCs, the defective region of spinal cord in rat fetuses with spina bifida aperta at E20 decreased obviously under stereomicroscopy, and the skin defect almost closed in many fetuses. The transplanted BMSCs in chitosan–gelatin scaffold survived, grew and expressed markers of neural stem cells and neurons in the defective spinal cord. In addition, the biomaterial presented high biocompatibility and slow biodegradation in vivo. In conclusion, prenatal transplantation of the scaffold combined with BMSCs could treat spinal cord defect in fetuses with spina bifida aperta by the regeneration of neurons and repairmen of defective region.     

气相色谱仪新旧检定规程的解读

本文主要从规程适用范围、计量器具控制、计量性能要求及检定方法等方面入手,全面的解读了气相色谱仪新旧检定规程的差异,为仪器检定及期间核查提供了参考。     

Implementation of pseudo-nondiffracting beams by use of diffractive phase elements

We report the experimental implementation of pseudo-nondiffracting beams by use of diffractive phase elements (DPE's). Based on the conjugate-gradient method presented in J. Opt. Soc. Am. A 15, 144-151 (1998), these DPE's are designed and fabricated on a flat quartz substrate. The experimental results show that the performance of the fabricated DPE's is in good agreement with the theoretical prediction.     

A network-based mobility management scheme for future Internet

The current Internet was originally designed for “fixed” terminals and can hardly support mobility. It is necessary to develop new mobility management schemes for the future Internet. This paper proposes an Identifiers Separating and Mapping Scheme (ISMS), which is a candidate for the future Internet mobility management, and discusses the basic principles and detailed message flow. ISMS is a network-based mobility management scheme that takes advantage of the identity and location separation. The mobility entities in the core network are responsible for the location management. ISMS is designed to satisfy the requirements of faster handover, route optimism, advanced management, location privacy and security. The average handover delay of ISMS is on the order of milliseconds only, which is far smaller than that of Mobile IPv6. Analyses show that ISMS can reduce packet overhead on wireless channels. We build a prototype and perform some experiments. Results verify the feasibility of ISMS.     

Embedding multi-dimensional meshes into twisted cubes

The twisted cube is an important variant of the most popular hypercube network for parallel processing. In this paper, we consider the problem of embedding multi-dimensional meshes into twisted cubes in a systematic way. We present a recursive method for embedding a family of disjoint multi-dimensional meshes into a twisted cube with dilation 1 and expansion 1. We also prove that a single multi-dimensional mesh can be embedded into a twisted cube with dilation 2 and expansion 1. Our work extends some previously known results.     

未雨绸缪 防患未然——“柔性测试”助力泛华测控提供高可靠性测试系统

<正>毋庸置疑,"可靠性"一直是评价电子产品性能的关键词之一,尤其在军工、航天等领域,产品的可靠性更是具有举足轻重的地位。一个细小器件的故障引发系统重大事故的案例屡见不鲜。随着人们对可靠性的重要性认识的不断提高,业内对可靠性理论及可靠性设计的研究也不断深入。12月2日,由中国电子     

The effect of the aspect ratio on the hydrophobicity of microstructured polydimethylsiloxane (PDMS) robust surfaces

In this study, we have designed and fabricated robust hydrophobic surfaces that are composed of various micropillar arrays and investigated the effect of the aspect ratio (feature height/feature size) of the micropillar on the wettability of the fabricated surfaces. The robust, micropillar-arrayed surfaces were designed to yield the same Wenzel and Cassie water contact angles (CAs). According to our design rule, one can achieve an enhanced hydrophobic surface by increasing the height of the micropillars. The designed hydrophobic surfaces were fabricated by polydimethylsiloxane (PDMS) replica molding with photolithographically micropatterned SU-8 masters. The hydrophobicity properties of the fabricated PDMS surfaces were fully characterized theoretically and experimentally. From the theoretical and experimental results, it was found that the micropillars of an intrinsically hydrophobic material with a high aspect ratio enhance the hydrophobicity of the surface by increasing the surface roughness (in view of the Wenzel state) and the opportunities for the entrapment of air beneath a water droplet (the Cassie state).     

Optimization of optical lens-controlled scanning electron microscopic resolution using generalized regression neural network and genetic algorithm

A scanning electron microscope (SEM) is a sophisticated equipment employed for fine imaging of a variety of surfaces. In this study, prediction models of SEM were constructed by using a generalized regression neural network (GRNN) and genetic algorithm (GA). The SEM components examined include condenser lens 1 and 2 and objective lens (coarse and fine) referred to as CL1, CL2, OL-Coarse, and OL-Fine. For a systematic modeling of SEM resolution (R), a face-centered Box–Wilson experiment was conducted. Two sets of data were collected with or without the adjustment of magnification. Root-mean-squared prediction error of optimized GRNN models are GA 0.481 and 1.96×10^-12 for non-adjusted and adjusted data, respectively. The optimized models demonstrated a much improved prediction over statistical regression models. The optimized models were used to optimize parameters particularly under best tuned SEM environment. For the variations in CL2 and OL-Coarse, the highest R could be achieved at all conditions except a larger CL2 either at smaller or larger OL-Coarse. For the variations in CL1 and CL2, the highest R was obtained at all conditions but larger CL2 and smaller CL1.