From Natural Methylation to Versatile Alkylations Using Halide Methyltransferases

Halide methyltransferases (HMTs) enable the enzymatic synthesis of S-adenosyl-l -methionine (SAM) from S-adenosyl-l -homocysteine (SAH) and methyl iodide. Characterisation of a range of naturally occurring HMTs and subsequent protein engineering led to HMT variants capable of synthesising ethyl, propyl, and allyl analogues of SAM. Notably, HMTs do not depend on chemical synthesis of methionine analogues, as required by methionine adenosyltransferases (MATs). However, at the moment MATs have a much broader substrate scope than the HMTs. Herein we provide an overview of the discovery and engineering of promiscuous HMTs and how these strategies will pave the way towards a toolbox of HMT variants for versatile chemo- and regioselective biocatalytic alkylations.     

Preparation,Properties and Applications of the Hybrid Organic/Inorganic Nanocomposite Based on Nanoporous Carbon Matrix

Journal of Inorganic and Organometallic Polymers and Materials - Nanoporous carbon matrix was prepared by the sol–gel process from pyrogallol-formaldehyde (PF) mixtures in water using picric...     

高强度高压绝缘子产品质量同其结构性能的重要关系(续)——制造者和用户应共同掌握的规则和指标(第二部分)

6　各种电瓷结构分析实例证实结构的差别下面介绍各种电瓷结构分析的结果。从扫描电镜照片 (ＲＥＭ)的判读中可见到这些材料的差别。试样ａ:日本瓷绝缘子Ｃ1 1 0 /1 1 2 ,制造年代 1 997试样ｂ :西门子瓷绝缘子Ｃ1 2 0 ,Ｃ1 3 0 ,制造年代 2 0 0 1试样ｃ:高强度矾土瓷绝缘子 ,西门子 2 0 0 1开发陶瓷岩相试样的制备系经多级金刚石研磨和抛光以及化学浸蚀。为判定气孔状态 ,对磨片作了透光照片 ,在其光亮区放大倍数为 1 0 0∶1 ,而且扫描电镜照片放大倍数为2 0 0∶1。图 5ａ　试样ａ光片的ＬＭ(透光显微镜 )和ＲＥＭ(扫描电镜 )图象 ,放大倍…     

Compositional reasoning using intervals and time reversal

Interval Temporal Logic (ITL) is an established formalism for reasoning about time periods. We investigate some simple kinds of ITL formulas which have application to compositional reasoning and furthermore are closed under conjunction and the conventional temporal operator known both as “box” and “always”. Such closures help us modularly construct formulas from simple building blocks in a way which preserves useful compositional properties. The most important class considered here is called the 2-to-1 formulas. They offer an attractive framework for analysing sequential composition in ITL and provide the formal basis for most of the subsequent presentation. A key contribution of this work concerns a useful and apparently new and quite elementary mathematical theorem that 2-to-1 formulas are closed under “box”. We also use a natural form of time symmetry with 2-to-1 formulas. This extends known facts about such formulas by looking at them in reverse. An important example involves showing that 2-to-1 formulas are also closed under a variant of “box” for prefix subintervals rather than suffix ones. We then apply the compositional formulas obtained with time symmetry to analyse concurrent behaviour involving mutual exclusion in both Peterson’s algorithm and a new and more abstract one. At present, our study of mutual exclusion mainly serves as a kind of experimental “proof of concept” and research tool to develop and illustrate some of the logical framework’s promising features. We also discuss how time symmetry sometimes assists in reducing reasoning in ITL to conventional linear-time temporal logic.     

The allelopathic mechanisms of dominance in bracken (Pteridium aquilinum) in Southern California

Bracken,Pteridium aquilinum, exerts a strong dominance over associated plants throughout much of its worldwide range. Associated plants are often severely inhibited or even excluded from dense stands of the fern. This study investigated the various aspects of herb suppression in bracken stands and assessed the contribution of the various forms of interference between plants to the establishment and maintenance of bracken dominance. It was shown that competition for soil moisture, light, and nutrients could not account for the lack of herbs in bracken stands. Further, uniformity of soil pH, texture, water-holding capacity, and organic matter content ruled out variability in physical factors as a cause. Baiting and trapping experiments showed that the higher concentration of animal activity inside the bracken stands contributed significantly to the pattern of herb suppression, but only against select species. The maintenance of this pattern in the animal-free Santa Cruz Island stands indicates the importance of another factor, allelopathy. It was found that phytotoxins leached from the dead, standing bracken fronds with the first few rains of the wet season were largely responsible for herb suppression. These toxins were isolated in raindrip and from soil inside the fern stands. Removal of the fronds from the stand before the rains could leach them resulted in reinvasion by the herbs after several seasons, and, conversely, placing fronds over the herbs in the grassland brought about herb inhibition. A number of known allelopathic chemicals were tentatively identified from bracken leachates. The importance of the interaction of allelopathy with other factors of plant interference is illustrated by bracken.     

护肤品的功效性评价方法综述

现代科学技术,尤其是生物技术和计算机技术的飞速发展和广泛应用,给化妆品行业带来了全新的发展机遇,赋予化妆品以一定的功效性是新世纪化妆品发展的重要趋势.近20年来,人们对化妆品的要求已从洁肤、润肤为目的的基础护肤品向美白、祛皱为目的的功效性化妆品方向发展.     

A mixed boundary-value problem of elasticity with parabolic boundary

Summary The mixed boundary-values problem of elasticity with parabolic boundary when the prescribed displacement is parabolic has been reduced to the solution of the non-homogeneous Hilbert problem following the method of complex variable. The result has been compared with that obtained byParia [5] for parabolic boundary with prescribed constant displacement as a particular case.

---

Zusammenfassung Das gemischte Randwertproblem der Elastizitätstheorie wird für parabolische Berandung auf die Lösung des inhomogenen Hilbert-Problems unter Benützung der komplexen Methode zurückgeführt. Die Randverschiebung wird durch ein Polynom zweiten Grades dargestellt. Das Ergebnis wird mit dem vonParia [5] verglichen, das als Sonderfall konstante Verschiebungen am parabolischen Rand vorschreibt.

     

Approximation mit Exponentialsummen

Zusammenfassung Für die Approximation mit Exponentialsummen ist die beste Approximation imTschebyscheffschen Sinne eindeutig, abgesehen von bisher nicht erkannten Ausnahmen. Eindeutigkeit liegt dagegen immer bei der Anpassung mit positiven Exponentialsummen vor. Daß diese Familie eine andere Struktur hat als die bekannten Verallgemeinerungen linearer Funktionenfamilien, zeigt sich am Charakter des Alternantenkriteriums.

---

Summary In this paper we investigate the approximation by sums of exponentials in the sense ofChebyshev. The best approximation is shown to be unique apart from exceptions not previously recognized. However, there is always uniqueness, when only sums of positive exponentials are considered. This family has a structure which is different from that of other generalizations of linear families of functions. This feature is reflected in the criterion on the alternations of the error curve.

---

---

Kernforschungszentrum Karlsruhe, Institut für Neutronenphysik und Reaktortechnik     

Some theorems of the eddy-current theory

Contents In the paper, theorems for the electric vector potentialT and magnetic scalar potentialU are discussed, which occur in the boundary-value problems for eddy currents induced in a conducting block by an external magnetic field. Uniqueness of the solution is examined. An integral representation of the scalar potentialU is derived for a nonmagnetic conductor. It is shown that for a thin metallic shell the integrodifferential equation results from general formulas related to the block.

---

Einige Theoreme der Wirbelstromtheorie

Übersicht In der Arbeit werden drei Theoreme für das elektrische VektorpotentialT und das magnetische SkalarpotentialU beschrieben, die in den Randwertaufgaben für in einem metallischen Block durch das äußere magnetische Feld induzierte Wirbelströme auftreten. Es wird bewiesen, daß eine solche Randwertaufgabe eine eindeutige Lösung hat, wenn das Vektorpotential auf dem Rand gleich Null ist. Eine Integralrepräsentation des Skalarpotentials wird abgeleitet. Es wird gezeigt, daß die Integrodifferentialgleichung für eine dünne metallische Hülle aus allgemeinen, den Block betreffenden Formeln hervorgeht.

---

List of principal symbols e coordinate unit vector - H magnetic intensity - J current density - T electric vector potential - U magnetic scalar potential - permeability - conductivity - V boundary of regionV