Restricted proteolysis of legumin of broad beans: Effect on thermodynamic properties of aqueous solutions and interaction with ficoll

Thermodynamic properties of aqueous solutions of both native and modified legumin of broad beans (Vicia faba L.) have been examined. A restricted trypsin-induced proteolysis was used to modify protein structure. Evaluation of protein affinity to ficoll in aqueous solutions showed that modified protein possessed higher hydrophilicity. Thermodynamic properties of diluted solutions were used to predict the phase behaviour in concentrated systems containing protein and ficoll. At specific concentrations of native legumin, the system can separate in two phases, whereas in the case of modified protein the single-phase behaviour of the system was predicted for any concentrations of both components. The experimental data obtained in concentrated systems confirmed predictions of thermodynamic analysis of diluted solutions.     

Accidents,often the result of an ‘uncontrolled business process’—a study in the (Dutch) chemical industry

Often companies in the (petro‐) chemical industry claim that all possible countermeasures against potential accidents have been taken and therefore accidents are unforeseeable. In this paper we question this statement by analysing the pre‐warning signals (precursors) preceding a number of industrial accidents. 17 accidents that occurred in the (petro‐) chemical industry have been investigated by exploring FACTS, an accident database containing information about industrial accidents worldwide. This paper will demonstrate that the existence of precursor information could have been used to foresee and even prevent these accidents if a proper control action had been initiated. The accidents are analysed further, according to a control model, which was adapted from that of C. Argyris. It demonstrates the ineffectiveness of several elements of business process control loops and that the so‐called ‘double‐loop learning’ cycle is more important than the ‘single‐loop learning’ cycle if one considers safety improvement. Copyright © 2003 John Wiley & Sons, Ltd.     

Smart help for operator performance

Our emphasis in this paper has been on the knowledge-based and cognitive issues pertinent for improved interactions between workers and manufacturing computer systems. We believe that these techniques are as necessary and applicable to manufacturing as to any other vocation, especially as the difference in collar color of shop people becomes less noticeable in their increasing role as knowledge workers.     

A systematic transition to flexible manufacturing

The transition from basic automation to flexible manufacturing is an expensive and tedious procedure. It requires meticulous planning and almost clairvoyant forecasting to insure that the initial flexibility obtained is sufficient to allow growth and expansion in the future. This paper will suggest a systematic and methodological approach to achieve optimal flexibility and describe the present results of its application to an ongoing system transformation.

Conclusions based on existing levels of completion are presented along with identification of critical and non critical flexibility requirements. The considerations and steps taken are summarized in a procedural format which may then be applied to a wide variety of system transformations.     

A method to estimate the initial length of equine tendons

A procedure is described by which the length of a tendon at the onset of loading is determined objectively. The procedure includes the fitting of third-order polynomial functions on the load-elongation data. The onset of loading is detected by an increasing fit of the polynomial by selective data reduction of the initial part of the load-elongation curve. The procedure results in an objective and reproducible definition of the zero strain level of a tendon.     

Intelligent structuring and exploration of digital music collections

In this paper we present a general approach to the automatic content-based organization and visualization of large digital music collections. The general methodology consists in extracting musically and perceptually relevant patterns (‘features’) from the given audio recordings (e.g., mp3 files), using topology-preserving data projection methods to map the entire music collection onto two-dimensional visualization planes (possibly in a hierarchical fashion), and using a new display metaphor (the ‘Islands of Music’) to display the inherent structure of the music collection to the user. It is shown how arbitrary meta-data can be integrated into the visualization process, and how similarity according to different viewpoints can be defined and exploited. The basic methodology is briefly described, three prototype systems are presented, and a general discussion of the practical application possibilities of such technologies is offered.     

Improving user security behaviour

Many organisations suspect that their internal security threat is more pressing than their external security threat. The internal threat is predominantly the result of poor user security behaviour. Yet, despite that, security awareness programmes often seem more likely to put users to sleep than to improve their behaviour. This article discusses the influences that affect a user's security behaviour and outlines how a well structured approach focused on improving behaviour could be an excellent way to take security slack out of an organisation and to achieve a high return for a modest, low-risk investment.