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81.
The defensive chemistry of juliformian millipedes is characterized mainly by benzoquinones (”quinone millipedes”), whereas the secretions of the putative close outgroup Callipodida are considered to be exclusively phenolic. We conducted a chemical screening of julid secretions for phenolic content. Most species from tribes Cylindroiulini (15 species examined), Brachyiulini (5 species examined), Leptoiulini (15 species examined), Uncigerini (2 species examined), Pachyiulini (3 species examined), and Ommatoiulini (2 species examined) had non-phenolic, in most cases exclusively benzoquinonic secretions. In contrast, tribes Cylindroiulini, Brachyiulini, and Leptoiulini also contained representatives with predominantly phenol-based exudates. In detail, p-cresol was a major compound in the secretions of the cylindroiulines Styrioiulus pelidnus and S. styricus (p-cresol content 93 %) and an undetermined Cylindroiulus species (p-cresol content 51 %), in the brachyiulines Brachyiulus lusitanus (p-cresol content 21 %) and Megaphyllum fagorum (p-cresol content 92 %), as well as in an undescribed Typhloiulus species (p-cresol content 32 %, Leptoiulini). In all species, p-cresol was accompanied by small amounts of phenol. The secretion of M. fagorum was exclusively phenolic, whereas phenols were accompanied by benzoquinones in all other species. This is the first incidence of clearly phenol-dominated secretions in the Julidae. We hypothesize a shared biosynthetic route to phenols and benzoquinones, with benzoquinones being produced from phenolic precursors. The patchy taxonomic distribution of phenols documented herein supports multiple independent regression events in a common pathway of benzoquinone synthesis rather than multiple independent incidences of phenol biosynthesis.  相似文献   
82.
Selection technologies such as phage and ribosome display, which provide a physical linkage between genetic information and encoded polypeptide, are important tools for the engineering of proteins for diagnostic and therapeutic applications. We have recently described a selection strategy called covalent DNA display, in which individual proteins are covalently linked to the cognate encoding DNA template in separate droplets of a water-in-oil emulsion. We here report on the optimization of several experimental steps in covalent DNA display technology, such as the elution conditions and the PCR strategy used for the amplification of selected DNA templates. A PCR assembly strategy was developed, which allows the amplification of the DNA templates over repeated rounds of selection. In addition, we could demonstrate that approximately 50% of the DNA templates form a covalent adduct with the corresponding proteins in the compartments of a water-in-oil emulsion. In model selection experiments, differences in recovery efficiency <100 000 per round of selection could be observed when comparing a specific binding polypeptide with a binder of irrelevant specificity. Furthermore, the optimized protocol was successfully applied for the selection of single domain proteins, capable of specific binding to mouse serum albumin (MSA). A mutant derived from the SH3 domain of the Fyn kinase, with millimolar affinity to MSA, was affinity matured using covalent DNA display and yielded several MSA binding FynSH3 variants with dissociation constants in the 100 nM range.  相似文献   
83.
A phase diagram based on dielectric-permittivity-versus-temperature measurements and high-temperature X-ray diffractometry was proposed for 0.4Pb(Ni1/3,Nb1/3)O3- x PbZrO3-(0.6- x )PbTiO3 (0.2 lessthan equal to x lessthan equal to 0.32) relaxor-ferroelectric solid solution, and a morphotropic phase boundary that sharply bends toward zirconium-rich compositions was found. A spontaneous normal-to-relaxor ferroelectric transition was also observed when heating was performed for all the compositions tested near the morphotropic phase boundary. Additional considerations about previously published phase diagrams for Pb(Zn1/3,Nb2/3)O3-PbTiO3 and Pb(Mg1/3,Nb1/3)O3-PbTiO3 might lead to an extension of the presented diagram to these compositions.  相似文献   
84.
An intelligent wireless sensor network that is designed to save road lighting energy, while maintaining traffic safety, is presented. It consists of an array of nodes that may be installed on both sides of the road, being mutually separated by a few tens to a few hundreds of meters. Each node consists of (i) vehicle detection subsystem, (ii) wireless communications and control subsystem for message exchange between the neighboring nodes, and (iii) lighting subsystem. During the day, nodes are inactive, while during the night, they turn to an active mode enabling detection of passing vehicles and timely activation of road lighting. Upon detection of a passing vehicle, the node generates a wireless message. Neighboring nodes that receive the wireless message will turn on their road lighting. In order to prevent driver distraction, each lamp will be turned on at a safe distance ahead of the moving vehicle. In order to improve energy efficiency, the lights will be dimmed or turned off behind the passing vehicle. The proposed road lighting energy-saving (RLES) system, which combines advanced sensor and wireless communication technologies, is suitable for implementation both with existing high-intensity discharge (HID) lamps and new light-emitting diode (LED) lamps. Our analysis shows that the RLES system will pay off in less than a year when used with HID lamps, while pay-back period is less than two and a half years when used with LEDs.  相似文献   
85.
An automotive brake's performance results from the complex interrelated phenomena occurring at the contact of the friction pair. These complex braking phenomena are mostly affected by the tribochemical properties of the friction material's ingredients, the brake disc properties, and the brake's operating regimes. In this paper, the synergistic effects of the friction material's properties, defined by its composition and manufacturing conditions, and the brake's operating regimes on the disc brake factor C variation have been modelled by means of artificial neural networks. The influences of 26 input parameters, determined by the friction material composition (18 ingredients), its manufacturing conditions (5 parameters), and the brake's operating regimes (3 parameters) on the brake factor C variation, have been predicted. The neural model of the disc brake cold performance has been developed by training 18 different neural network architectures with the five different learning algorithms. The optimal neural model of disc brake operation has been shown to be valid for predicting the brake factor C variation of the cold disc brake over a wide range of brake's operating regimes and for different types of friction material.  相似文献   
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ABSTRACT

The measurement of acoustic emission (AE) signals during injection molding of polypropylene with new and damaged mold is presented. The damaged injection mold was fitted with a steel insert with cracks induced by laser surface heat treatment. Two resonant piezoelectric AE sensors were attached to the mold via AE waveguides. To improve the mold integrity prediction with smaller defects, AE signal frequency characteristics and a measure of AE signal amplitude probability distribution are implemented. A 5-dimensional feature vector with real-valued explanatory variables is proposed, providing the defining points in an appropriate multidimensional space to characterize the state of injection molding tool. Feature vectors are classified with neural network pattern recognition. The results confirm that presented AE technique offers characterizing the integrity of molds also with resonant sensors.  相似文献   
89.
In this study the formation of photo-oxidative species was studied by monitoring the changes in the FT-IR spectra and colour changes. To stabilize long-oil air drying alkyd paint, separate and synergistic influence of two stabilizers were used Uvasorb®S28 as the UV-absorber and Uvasorb®HA29 as the stabilizer which inhibits the degradation of some groups. In the presence of UV radiation (450 h, λ > 300 nm), the alkyd paint undergoes photodegradation with gradual change of its colour. The photochemical degradation of the alkyd paint is associated with an increase in the hydroxyl content and broadening of the absorption in the carbonyl region. Analysis of the colour changes in alkyd surface during photodegradation was carried out by measuring CIEL*a*b* colour components (L*, a*, b* and ΔE*). Overall, ΔE* colour change correlates well with photodegradation of alkyd paint by relative increase of the concentration of carbonyl and hydroxyl groups determinated by FT-IR measurements.  相似文献   
90.
The aim of this paper is to improve the G.711 standard, which is widely used, especially in the public switched telephone network (PSTN). Two solutions are proposed. The first solution uses only lossless coder, achieving a bit-rate decrease of 0.82 bits/sample, compared to the G.711 codec. The second solution uses forward adaptation and a lossless coder, further decreasing the bit-rate (by 1.25 bits/sample) and achieving higher average signal-to-quantization noise ratio (SQNR) in comparison with the G.711 codec. Also, the second solution is more robust than the G.711 codec, which means that it has near constant SQNR for a wide range of input signal power. That is very important for signals whose input power varies with time, such as speech and video signals. Our solutions are compatible with the G.711 codec, they have little additional complexity and delay and therefore can be applied in real-time systems, such as PSTN or VoIP. They can also be used in many other systems, such as WiMax and OFDM, as a replacement or improvement of the G.711 codec. Standardization process of the G.711.1 standard (which is a wide-band extension of the G.711 standard) is largely present. Our solutions fulfill all the requirements for that new standard; therefore they can be implemented in its low-frequency part.  相似文献   
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