Secreted HSP90α-LRP1 Signaling Promotes Tumor Metastasis and Chemoresistance in Pancreatic Cancer

The extracellular heat shock protein 90α (eHSP90α) has been reported to promote cancer cell motility. However, whether pancreatic cancer (PC) cells expressed membrane-bound or secreted HSP90α, as well as its underlying mechanism for PC progression, were still unclear. Our study demonstrated that the amounts of secreted HSP90α proteins were discrepant in multiple PC cells. In addition, highly invasive Capan-2 cells have a higher level of secreted HSP90α compared with those of less invasive PL45 cells. The conditioned medium of Capan-2 cells or recombinant HSP90α treatment stimulated the migration and invasion of PC cells, which could be prevented with a neutralizing anti-HSP90α antibody. Furthermore, secreted HSP90α promoted elements of epithelial–mesenchymal transition in PL45 cells, including increases in vimentin and Snail expressions, decreases in E-cadherin expression, and changes in cell shape towards a mesenchymal phenotype, but these phenomena were reversed by the anti-HSP90α antibody in Capan-2 cells. In addition, high levels of low-density lipoprotein receptor-related protein 1 (LRP1) were associated with worsened patient survival in pancreatic adenocarcinoma. We demonstrated LRP1 as a receptor of eHSP90α for its stimulatory role in metastasis, by activating the AKT pathway. In addition, silencing LRP1 enhanced the chemosensitivity to gemcitabine and doxorubicin in Capan-2 cells. Therefore, our study indicated that blocking secreted HSP90α underlies an aspect of metastasis and chemoresistance in PC.     

异相膜NIMTE在空气能量回收装置中使用效果试验研究

通过对国产纸膜和日本滤纸膜材料的分析研究,研制出NIMTE异相膜,并在空气能量回收装置使用过程中对风压、空气流量、热流方向与热交换效率、回收能量、能效及安全性的关系进行分析,设计出能够更好发挥异相膜使用效果的传热膜机芯,有效提高空气能量回收装置的综合性能。     

我国混凝土空心砌块节能建筑案例分析

通过我国混凝土空心砌块节能建筑案例分析,提出外保温、内保温、夹芯保温各有优缺点,应因地制宜,依据建筑功能要求采取适合的保温方式,并根据多年的研究和应用实践经验,提出了我国不同气候区域混凝土空心砌块节能建筑的外墙构造设计方案。     

Chl1, an ATP-Dependent DNA Helicase,Inhibits DNA:RNA Hybrids Formation at DSB Sites to Maintain Genome Stability in S. pombe

As an ATP-dependent DNA helicase, human ChlR1/DDX11 (Chl1 in yeast) can unwind both DNA:RNA and DNA:DNA substrates in vitro. Studies have demonstrated that ChlR1 plays a vital role in preserving genome stability by participating in DNA repair and sister chromatid cohesion, whereas the ways in which the biochemical features of ChlR1 function in DNA metabolism are not well understood. Here, we illustrate that Chl1 localizes to double-strand DNA break (DSB) sites and restrains DNA:RNA hybrid accumulation at these loci. Mutation of Chl1 strongly impairs DSB repair capacity by homologous recombination (HR) and nonhomologous end-joining (NHEJ) pathways, and deleting RNase H further reduces DNA repair efficiency, which indicates that the enzymatic activities of Chl1 are needed in Schizosaccharomyces pombe. In addition, we found that the Rpc37 subunit of RNA polymerase III (RNA Pol III) interacts directly with Chl1 and that deletion of Chl1 has no influence on the localization of Rpc37 at DSB site, implying the role of Rpc37 in the recruitment of Chl1 to this site.     

浅谈零缺陷与质量成本

阐述零缺陷在工作中的行动准则和零缺陷管理所要达到的最终目标,分析了零缺陷在实际生产中对质量成本的影响。     

典型脱硫工艺在火电厂的应用探讨

我国电力生产的水电为主,在全国发电量中火电占80%左右,而火电中90%为燃煤机组,燃煤电厂是污染大户,是酸雨及二氧化硫污染的主要来源之一.随着我国政府对酸雨及二氧化硫污染控制的高度重视,火电厂二氧化硫最高允许排放浓度和两控区内火电厂二氧化硫总量控制标准的实施.     

Effect of deteriorated microstructures on stress corrosion cracking of X70 pipeline steel in acidic soil environment

In order to investigate stress corrosion cracking (SCC) of X70 pipeline steel and its weld joint area in acidic soil environ-ment in China, two simulating methods were used: one was to obtain bad microstructures in heat affected zone by annealing at 1300°C for 10 min and then, quenching in water; the other was to get different simulating solutions of acidic soil in Yingtan in south-east China. The SCC susceptibilities of X70 pipeline steel before and after quenching in the simulating solutions were analyzed...     

出钢顺立案模型在宝钢炼钢厂的应用

从基本概念和程序的具体实现两方面,阐述了出钢顺立案模型的功能、特点以及在生产实践中所取得的效果。     

L-Fuzzy拓扑空间中的半内部、半闭包、半边界、半正则性质

在Ｌ－Ｆｕｚｚｙ拓扑空间中给出了半内部、半闭包、半边界和半正则的定义，讨论了它们的性质，并借助于它们给出了半开集、半闭集、正则开（闭）集的等价刻划．