Nested polymerase chain reaction (PCR) and multiple cloned antibody capture PCR for the examination of serum hepatitis B virus DNA in negative hepatitis B surface antigen patients suffering from liver diseases

In order to find out rapidly the causes of the liver diseases suffered by patients with negative hepatitis B surface antigen (HBsAg), nested polymerase chain reaction (PCR) and multiple cloned antibody capture PCR techniques were established to examine serum hepatitis B virus (HBV) DNA. By using both techniques along with the examination of hepatitis C virus (HCV) infection, the causes of chronic liver diseases with negative HBsAg were studied. It is found that nested-PCR can increase the sensitivity of single PCR more than 1,000 fold and multiple cloned antibody capture-PCR can detect concentration of HBV DNA as low as 0.1-0.01 pg/L. HBV DNA positive patients were found in 45.5%, 30.8%, 13.3% and 100% respectively of the patients suffering from liver cirhosis with negative HBsAg (group A, 22 cases), chronic hepatitis with negative HBsAg (group B, 13 cases), normal subjects with negative HBsAg and positive hepatitis B core antibody (HBcAb, group C, 30 cases) and liver cirhosis with positive HBsAg and negative HBeAg (group D, 12 cases). HBV DNA can be also found in the serum of HBsAb positive patients and subjects supposed to be healthy, 81.8% and 53.8% of the patients were infected with HBV and/or HCV in group A and group B respectively. All these results suggest that nested-PCR and multiple cloned antibody capture-PCR are rapid and highly sensitive methods for detection of serum HBV DNA. HBV infection is an important cause of chronic liver diseases in patients with negative HBsAg. The causes of most of the HBsAg-negative chronic liver diseases are related with infection of viruses. The clinical significance of serum HBsAb in naturally infected patients should be reconsidered.     

利用射线路径反演层状介质速度的方法

本文根据欧拉方程对于连续层状介质进行了方程推导，得出一个波传播的射线路径公式，利用中 公式导出了ＶＳＰ资料处理中的速度反演公式。     

一种按时间切换的最速控制方法

基于初始状态空间下的子空间概念，提出了一种按时间进行切换的最速控制方法，不需要求取开关线或开关面。数字仿真结果表明，采用这一方法是完全可行的。     

基于功能的数据箱管理系统的设计

本文介绍数据箱库ＢＯＸＤＢ的模型及数据箱结点结构，并给出它的数据管理方法，方法是一组以Ｃ编写的专用库函数。     

矩形橡胶复合材料层合板几何非线性分析

文中应用简单高阶剪切层合理论和大变形理论对各向异性纤维增强的矩形橡胶复合材料层合板进行了几何非线性分析。与线性一理论相比，本文作者进行的层合板大变形状态的几何非线性分析和所计算的层板应力分布，更准确性。     

Microphase structure and calculations of the interphase in poly (styrene-b-methylphenylsiloxane) diblock copolymers: a comparative study from small-angle X-ray scattering,electron spectroscopic imaging and solid-state nuclear magnetic resonance

The microphase structure of a series of poly (styrene-b-methylphenylsiloxane) (PS-b-PMPS)-diblock copolymers with various compositions and molecular weights has been studied by small-angle X-ray scattering, electron spectroscopic imaging and solid-state nuclear magnetic resonance spectroscopy. This diblock copolymer system is miscible within a distinct range of temperature and molecular weight exhibiting an upper critical solution temperature. The three chosen methods which complement each other can be applied to the chemically unmodified model system. The results of the various measurements correlate to describe the microphase structure, the domain size and the interphase.     

Plasma Asp13-Ki-ras oncoprotein expression in vinyl chloride monomer workers in Taiwan

Vinyl chloride (VC) workers are known to be at risk for development of liver angiosarcoma, a rare tumor. Previously, more than 80% of VC workers with liver angiosarcoma have been found to have an Asp-13 c-Ki-ras oncogene mutation, and more than 50% of VC-exposed workers without liver tumors were found to have Asp13-Ki-ras oncoprotein in their plasma. Some workers in Taiwan had also been exposed to VC, and some have contracted liver tumors. In this study, we used enhanced chemiluminescence Western blotting to detect Asp13-p21-Ki-ras in the sera of VC-exposed workers in Taiwan. There were 14 of 113 (12.4%) VC workers positive for the Asp13-Ki-ras oncoprotein in plasma, but 0 of 18 controls were positive. There were 10 of 69 (14.5%) plasma-positives among the more highly exposed (> 1000 ppm-months) workers and 4 of 48 (9.1%) plasma-positives among the lesser exposed (< or = 1000 ppm-months). Compared with the unexposed controls, the odds ratios (and 95% confidence intervals [CI]) for plasma-positivity were 4.11 (95% CI = 0.21, 80.4) in the lower-exposed workers and 6.53 (95% CI = 0.37, 116.9) in the higher-exposed workers, and there was a linear trend between exposure and plasma-positivity (P = 0.073). After adjusting for age and drinking status, the odds ratios (and 95% CIs) were 1.64 (95% CI = 0.17, 15.8), and 2.65 (95% CI = 0.42, 16.8), respectively, and there was a significant linear trend between exposure and plasma-positivity (P = 0.048). In summary, Asp13-Ki-ras oncoprotein can be found in the plasma of VC workers in Taiwan, and a significant dose-response relationship exists between plasma oncoprotein expression and VC exposure.     

威远构造与震旦系油气勘探

本文扼要地论述了威远构造从30年代到60年代震旦系最古老气藏发现的漫长历史,以及直到90年代初震旦系天然气勘探重新获得突破的曲折过程。这一历史过程,也是四川盆地复杂碳酸盐岩裂缝性气藏勘探的剪影。     

电流变体特性及电流变体应用器件的进展

本文详细评述了电流变体(ERF)材料的组成、工作机理及其流变力学性能,提出以Bingham塑性体来近似描述电流变体的流变特性,综述了电流变体在智能材料、汽车离合器、液压阀门等器件中的应用,给出了含电流变体的双层智能复合材料梁单层、双层加电状态对其振动特性的影响,设计了含电流变体的主动控制式减振器,并对其在外加电场作用下的减振性能进行了评价。     

建筑群体综合控爆技术

本项工程采用铵梯炸药、水胶炸药、毫秒延期电雷管等爆破器材，运用微差起爆，定向折叠，水压爆破等控制爆破技术，成功地拆除了总面积达３８００ｍ２的建筑群，达到了爆破噪声低，震动小，碎片坍塌范围小，尘土飞扬少等原设计目标。