A novel fluorescent label based on organic dye-doped silica nanoparticles for HepG liver cancer cell recognition

In this paper, we report a method for the recognition of HepG liver cancer cells with the use of a novel fluorescent label based on organic dye-doped fluorescent silica nanoparticles. The novel organic dye-doped silica nanoparticles are prepared with a water-in-oil microemulsion technique. The silica network is produced by the controlled synchronous hydrolysis of tetraethoxysilane and 3-amino-propyltriethoxysilane (APTES). The organic dye fluorescein isothiocyanate is doped inside as a luminescent signaling element, through covalent bonding to the amino group of APTES. The organic dye-doped core-shell nanoparticles are highly luminescent and exhibit minimal dye leaching and excellent photostability. A novel fluorescent label method based on biological fluorescent nanoparticles has been developed. The dye-doped fluorescent silica nanoparticles are covalently immobilized with anti-human liver cancer monoclonal antibody HAb18. We have used antibody-labeled fluorescent nanoparticles to recognize HepG liver cancer cells. It has been observed that the bioassay based on the organic dye-doped nanoparticles can identify the target cells selectively and efficiently. The fluorescent nanoparticle label also exhibits high photostability.     

晚三叠世末期楚雄盆地云龙凹陷海相沉积的分子地球化学证据

在楚雄盆地云龙凹陷山品村和背阴山上三叠统剖面泥质烃源岩中检出较丰富的24-正丙基C30甾烷和4-甲基C30甾院。“三芴”系列分布以二苯并噻吩绝对占优为特征,除二苯并噻吩外,还检出高含量的苯并萘噻吩系列化合物。这表明．在晚三叠世未期,云龙凹陷海相沉积环境是客观存在的。图3表1参16     

Pressurized electrochromatography coupled with electrospray ionization mass spectrometry for analysis of peptides and proteins

Pressurized capillary electrochromatography (pCEC) was coupled with electrospray ionization mass spectrometry (ESI-MS) using a coaxial sheath liquid interface. It was used for separation and analysis of peptides and proteins. The effects of organic modifier and applied voltage on separation were investigated, and the effects of pH value of the mobile phase and the concentration of the electrolyte on ESI-MS signal were investigated. The resolution and detection sensitivity with different separation methods (pCEC, capillary high-performance liquid chromatography) coupled on-line with mass spectrometry were compared for the separation of a peptide mixture. To evaluate the feasibility and reliability of the experimental setup of the system, tryptic digests of cytochrome c and modified protein as real samples were analyzed by using pCEC-ESI-MS.     

Modeling multiaxial impact behavior of a glassy polymer

In many applications of polymers, impact performance is a primary concern. Impact tests experimentally performed on molding prototypes yield useful data for a particular structural and impact loading case. But, it is generally not practical in terms of time and cost to experimentally characterize the effects of a wide range of design variables. A successful numerical model for impact deformation and failure of polymers can provide convenient and useful guidelines on product design and therefore decrease the disadvantages that arise from purely experimental trial and error. Since the specimen geometry and loading mode for multiaxial impact test provides a close correlation with practical impact conditions and can conveniently provide experimental data, the first step of validating a numerical model is to simulate this type of test. In this paper, we create a finite element analysis model using ABAQUS/Explicit to simulate the deformation and failure of a glassy ABS (acrylonitrile-butadiene-styrene) polymer in the standard ASTM D3763 multiaxial impact test. Since polymers often exhibit different behavior in uniaxial tensile and compression tests, the uniaxial compression or tensile tests are generally not representative of the three-dimensional deformation behavior under impact loading. A hydrostatic pressure effect (controlled by the parameter γ) is used to generalize a previously developed constitutive model ("DSGZ" model) so that it can describe the entire range of deformation behavior of polymers under any monotonic loading modes. The generalized DSGZ model and a failure criterion are incorporated in the FEA model as a user material subroutine. The phenomenon of thermomechanical coupling during plastic deformation is considered in the analysis. Impact load vs. displacement and impact energy vs. displacement curves from FEA simulation are compared with experimental data. The results show good agreement. Finally, equivalent stress, strain, strain rate and temperature distributions in the polymer disk are presented. Electronic Publication     

Special decentralized control problems in discrete-time interconnected systems composed of two subsystems

In this paper, some special decentralized control problems are addressed for discrete-time interconnected systems. First it is pointed out that some subsystems must be unstable to ensure stability of the overall system in some special cases. Then a special kind of decentralized control problem is studied. This kind of problem can be viewed as harmonic control among independent subsystems. Research results show that two unstable systems can generate a stable system through some effective cooperations. In addition, a decentralized controller design method based on linear matrix inequality is also given by using parameter-dependent Lyapunov function method developed for the study of robust stability. A special linear star coupled dynamical network is also considered. The central subsystem must be unstable to stabilize the whole network under a special coupling. Several examples are given to illustrate the results.     

一种数据采集器软件设计与实现

文章介绍了监控类数据采集器的软件设计与原理.着重讨论了一种监控类通用数据采集器软件的实现技术和方法.     

天钢物资计量计算机网络系统

介绍了天津钢铁有限公司物资计量计算机网络系统。该系统具有安全性高，数据可追溯性好，多种报警提示功能等特点。采用该系统后，加快了物资信息传递速度，规范了企业管理流程，提高了物流速度。     

高温超导带材剩余磁场非接触无损连续测量方法及装置

通过对高温超导带材内部剩余磁场的连续测量，计算机采集数据，并依据超导临界态理论模型计算出临界电流值。为Bi系高温超导带材提供了一种非破坏的连续检测手段，不仅实现了超导带材剩余磁场的实时显示，而且可以给出超导带材均匀性的信息，最终能够计算出整根超导带材临界电流的平均值和不确定度。     

认知无线电的关键技术和应用研究

认知无线电是一种新的智能无线电技术,它通过动态的接入频谱为用户提供高容量的服务,能极大的改善现有的低效的频谱利用率。文章重点分析了认知无线电中频谱检测、频谱管理、功率控制等关键技术,以及认知无线电在超宽带、Mesh网、无线区域网的应用现状,最后探讨了认知无线电发展需要关注的难点问题。