Three-Dimensional Visualization of Transgenic Tobacco Leaves

We have done a three-dimensional visualization of transgenic tobacco (Nicotiana tabacum) leaves for the study of chloroplast gene expression and regulation. The aim was to visualize tobacco leaves shot with tungsten particles. These tungsten particles were coated with the foreign DNA and shot into the leaf using the biolistic technique of DNA insertion. The visualization can be used to examine the leaves to gauge the efficiency of the shooting process, i.e.,to see what parts of the leaves have been effectively penetrated by the DNA-coated tungsten particles and also to judge the depth of penetration. The image data for the 3D visualization was collected at planes 10 microns apart, using a prototype version of a High Numerical Aperture Reflecting Microscope. The raw image data collected from the microscope was restored using the Row Action Projection (RAP) algorithm and the Partial Minimization and Constrained Iteration (PCMI) algorithm. These restored images were then used for 3D visualization using the Visualization Toolkit.     

An excimer-pumped β-BaB2O4 opticalparametric oscillator tunable from 354 nm to 2.370 μm

A pulsed singly resonant optical parametric oscillator (OPO) has been constructed which can provide tunable coherent radiation over the entire wavelength range extending from 354 nm in the near ultraviolet, throughout the visible, to 2.370 μm in the near infrared using a single β-BaB₂O₄ crystal. The oscillator is pumped at 308 nm by a pulsed narrow band injection-seeded XeCl excimer laser in a near-field pumping configuration, and energy conversion efficiencies (optical-to-optical) in excess of 10% have been obtained in a 12-nm-long crystal across the OPO tuning range. The requirements placed upon the spectral and spatial coherence of the pump laser for optimum OPO operation are discussed. Experimental results on several oscillator parameters, including tuning range, oscillation threshold, energy conversion efficiency, and spatial and temporal characteristics, are presented     

Housing and Health Inequalities: Review and Prospects for Research

To date, relatively little research has systematically investigated relationships and pathways between housing, socio-economic status, and health status. At the same time, there is a growing awareness that one of the most important research needs in health inequalities scholarship is to better elucidate those pathways by which differences in socio-economic status manifest in everyday life, and produce, at the aggregate level, the systematic social gradient in health observed in all industrialised countries of the world. Existing 'population health' research on health inequalities provides evidence of the influence of four key factors upon health: social support, workplace organisation, income inequalities, and life course factors. This material is reviewed in the first section of the paper. The second section reviews the existing literature on housing and health, and finds little work that explicitly investigates housing as a factor in the social production of health inequalities. By extension and analogy, the final section shows, each of the four emphases in health inequalities can be translated into a fruitful, substantive research issue for housing and health research.     

A comparison of the effectiveness of malignancy detection in body fluid examination by the cytopathology and hematology laboratories

BACKGROUND: Body fluid specimens in many institutions are submitted for cytologic examination as well as for examination in a clinical microscopy or hematology laboratory. The cytology laboratory is generally seen as the standard for detection of malignancy, whereas the clinical microscopy laboratory is often depended on predominantly for cell counting and categorization. METHODS: To analyze the effectiveness of the hematology laboratory at detecting malignant fluids, this study retrospectively analyzed reports on 397 body fluid specimens (cerebrospinal, pericardial, peritoneal, and pleural) that were concurrently submitted over a 12-month period to both the cytopathology laboratory and the hematology laboratory. RESULTS: Thirty-seven (9.3%) of the cases were diagnosed as malignant by at least one of the two examinations. The cytopathology examination reported 27 (73%) of the 37 malignant cases as malignant and 30 (81.1%) as at least atypical (27 malignant and 3 inconclusive), and the hematology examination reported 34 (91.9%) as malignant and 36 (97.3%) as at least atypical. A concordant malignant diagnosis was given by both laboratories in 24 (64.9%) of the 37 cases. CONCLUSIONS: These results show that examination of specimens by the hematology laboratory can provide a highly sensitive diagnostic evaluation in addition to its more customary role of providing timely cell counts.     

The evaluation of corrosion processes for engineered barrier systems

The U.S. Nuclear Regulatory Commission (NRC) established a corrosion program to evaluate the predicted performance of the U.S. Department of Energy’s proposed engineered barrier system for the potential high-level waste geologic repository at Yucca Mountain, Nevada. The risk-informed, performance-based regulation established by the NRC for the potential licensing of the proposed repository is introduced in this article with a discussion of resolution methods for various technical issues. These resolution methods include probability or consequence screening, deterministic confirmatory research investigations, and the use of the Total-System Performance Assessment Code to determine the risk significance of specific materials degradation processes on the total system performance. An example of the deterministic analysis method is the evaluation of the susceptibility of mill-annealed and gas-tungsten-arc welded alloy 22 to lead-assisted stress corrosion cracking in deaerated PbCl₂ and Pb(NO₃)₂ solutions at 95°C. Also in this article is an evaluation of the risk significance of high-temperature deliquescence-induced localized corrosion of the alloy 22 waste package outer barrier on the total system performance. Author’s Note: This paper was prepared to document work performed by the Center for Nuclear Waste Regulatory Analyses (CNWRA) for the Nuclear Regulatory Commission (NRC) under Contract No. NRC-02-02-012. The activities reported here were performed on behalf of the NRC Office of Nuclear Material Safety and Safeguards, Division of High-Level Waste Repository Safety. This paper is an independent product of the CNWRA and does not necessarily reflect the view or regulatory position of the NRC. The NRC staff views expressed herein are preliminary and do not constitute a final judgment or determination of the matters addressed or of the acceptability of a license application for a geologic repository at Yucca Mountain. For more information, contact A. Csontos, U.S. Nuclear Regulatory Commission, Office of Nuclear Material Safety & Safeguards, Washington, D.C. 20555-0001; (301) 415-6352; fax (301) 415-5397; e-mail aac@nrc.gov.     

Evaluation of the conduction shape factor with a CFD code for a liquid–metal heat transfer in heated triangular rod bundles

A heat transfer due to conduction through a coolant itself is not negligible in a liquid–metal cooled reactor (LMR). This portion of a heat transfer is frequently described with a conduction shape factor during the thermal-hydraulic design of an LMR. The conduction shape factor, which is highly dependent on a pitch-to-diameter (P/D) ratio, is defined as the ratio of the local conduction heat flux at a gap between two subchannels to the reference heat flux calculated by the averaged subchannel temperatures. The shape factors in heated triangular rod arrays for three different pitch-to-diameter ratios are generated through CFX calculations in the present study. The flow paths of 1.0–2.0 m in length are meshed into 180,000–360,000 volumes depending on the flow velocities. The SSG Reynolds stress model is used as a turbulent model in the calculations. The evaluated data fell between the heated-rod data and the plane-source data obtained by theoretical investigations. The conduction shape factors were found to be independent of the heating pattern of the rod arrays. Based on the evaluated data, a correlation for a liquid sodium coolant is suggested, which will improve the accuracy of the subchannel analysis codes for the thermal-hydraulic design of an LMR. When it is compared with the existing correlations, the suggested correlation is expected to enhance the reliability of the conduction shape factor because the data is evaluated by a more realistic numerical experiment.     

Investigation of ammonium polyphosphate dilution with ground eggshells and lignin through the study of natural fibre composite flammability

The interactions of Calcium carbonate (e.g., eggshell powder) and Lignin with ammonium polyphosphate (APP) when used as fire retardants were investigated. Three mixing ratios - 1:3, 1:1, and 3:1, were used with natural fibre reinforced composites containing a hemp mat and an epoxy matrix manufactured using a light resin transfer moulding (L-RTM) process. The thermal decomposition of the retardant mixtures and composites was investigated using thermogravimetric analysis (TGA). The findings showed that even though the decomposition reactions of APP with eggshell powder and lignin mixtures interacted and overlapped, the same interactions could not be seen in the composites. In the composite form while the residue was affected by the retardant, the decomposition reactions were driven primarily by the hemp and epoxy. Flammability of the composites was studied by testing to 20, 35, 50, and 75 kW/m² with a cone calorimeter, and determining the critical heat flux. While the samples with eggshell powder had higher ignition times, the critical heat flux for ignition was 13 kW/m² for all sample groups except for a ratio of 1:3 APP to eggshell powder, which was 14 kW/m². The lowest burning rates (mass loss and heat release) occurred in composites containing only APP, however, the addition of eggshell powder or lignin at even a ratio of 3:1 APP to either provided a notable reduction.     

Case study of attempts to enact self service tobacco display ordinances: a tale of three communities

OBJECTIVE: To examine self service tobacco displays (SSTDs) and youth retail tobacco access by comparing longitudinal illegal tobacco sales rates in three communities in Santa Barbara County, California, that considered or implemented ordinances banning SSTDs. A confirmatory survey was also conducted to substantiate the longitudinal data. DESIGN: A longitudinal case study design was utilised. Five undercover tobacco buys were conducted between 1994 and 1997 (n = 332). In addition, one confirmatory survey was conducted in a geographically separated area, which had no ordinances banning SSTDs (n = 57). RESULTS: Decreases in youth buy rates were reported in all three communities. Most notably, the first city to enact a SSTD ban, Carpinteria, achieved a 0% sales rate, which was maintained throughout the study period. In contrast, Santa Barbara and Goleta experienced considerable drops in their illegal sales rates, but neither community obtained results as dramatic as those found in Carpinteria. The confirmatory survey showed that 32.1% of stores with SSTDs sold cigarettes to minors; this compares to a sales rate of 3.4% in stores without SSTDs (chi(2) (1) = 8.11, p = 0.004). CONCLUSIONS: Efforts to enact self service bans are likely to meet with retail and tobacco industry opposition, as was the case in this study's three communities. The process of community debate, resultant publicity surrounding the issue, and enactment of SSTD ordinances may serve to not only increase merchant awareness of youth tobacco laws and their penalties but also may contribute to reduced youth cigarette sales rates. Implications and limitations of the findings are discussed.