Sol-gel Preparation and Preliminary in vitro Evaluation of Fluorapatite／Hydroxyapatite Solid Solution Films

Fluorapatite/hydroxyapatite solid solution has better biological properties than other apatites, especially used as films or coatings. In this work, sol-gel preparation and in vitro behavior of fluorapatite/hydroxyapatite solid solution films on titanium alloy were investigated. Ca(NO3)2-4H20 and PO(OH)x(OEt)3-x were selected as precursors, and hexafluorophosphoric acid (HPFo) was used as a fluorine containing reagent. The Ca and P precursors were mixed with HPFo to keep the Ca/P molar ratio 1.67. The mixtures refluxed for 12 h were used as dipping sols for the preparation of the films. The phase of the films obtained at 600℃ was apatite. The F contents in the films increased with the concentrations of HPF6 in the dipping sols. The solid solution films were shown to have better stability than hydroxyapatite films, and a reasonably good bioactivity in the in vitro evaluation.     

Analysis of laminated doubly-curved shells by a layerwise theory and radial basis functions collocation,accounting for through-the-thickness deformations

In this paper, the static and free vibration analysis of laminated shells is performed by radial basis functions (RBFs) collocation, according to a layerwise deformation theory (LW). The present LW theory accounts for through-the-thickness deformation, by considering an Mindlin-like evolution of all displacements in each layer. The equations of motion and the boundary conditions are obtained by Carrera’s unified formulation, and further interpolated by collocation with RBFs.     

Admixtures effect on fresh state properties of aerial lime based mortars

Especially in rehabilitation works, the use of lime based mortars have been increasing due to the need of compatibility between the old and the new materials. The mortars fresh state properties are extremely important since determines the material workability and also have a great influence on its hardened state characteristics.In this work, the fresh state properties of aerial lime pastes and aerial lime based mortars were investigated. The torque variation with time and the mortar rheological parameters (relative yield stress and plastic viscosity) were obtained using a rheometer suitable for mortars. The correlation of rheological data with slump and relative density measurements was studied.The mortars workability is affected by several parameters, namely, the binder/aggregate and water/binder ratios, the kneading water content, the admixtures type and amount. The admixtures influence (type and amount) on the fresh state properties of aerial lime based mortars are discussed. The used admixtures were the most common ones for several mortars, such as a water-retaining agent, a plasticizer and an air-entraining agent.     

Electric distribution network reconfiguration based on a fuzzy multi-criteria decision making algorithm

Topological reconfiguration is an important tool for the planning and operation of electric power distribution systems. The amount of time that an algorithm spends to obtain an alternative topological status for the system is not a primary concern, as the main goals of distribution operation planning are the reduction of power losses, the enhancement of the voltage profile, and the increase of reliability levels. The utility can use multiple criteria regarding the observation of regulation policies and public awareness to drive the topological reconfiguration. Several researchers are looking for new optimization methods, as the complexity of this combinatorial issue is high in large systems and the classic optimization methods are failing to address the problem reasonably. Therefore, a new fuzzy multi-criteria decision making algorithm for the proper processing of the information sources available at the utilities in the context of distribution network reconfiguration is proposed. The algorithm is evaluated through the use of a proof-of-concept implementation in a set of case studies based on actual distribution systems.     

Burial grounds’ impact on groundwater and public health: an overview

The most common practice for disposal of dead bodies is inhumation in soil, which favours interactions with the surrounding environment and returns nutrients to the life cycle. However, when the burial ground is located where hydrogeological, geological and climatic conditions are not favourable to the process, contamination of soils and groundwater may occur, and decomposition may be inhibited, leading to social, economic and political problems. The most critical parameters when assessing the pollution potential of a burial ground are inhumation depth, geological formation, depth of the water table, density of inhumations, soil type and climate. Considering that, this paper presents an overview of the potential threat that cemeteries can pose, analysing and discussing the influence of the main variables causing environmental impacts and public health risks.     

Short-Term Immune Responses of Gilthead Seabream (Sparus aurata) Juveniles against Photobacterium damselae subsp. piscicida

Photobacteriosis is a septicaemic bacterial disease affecting several marine species around the globe, resulting in significant economic losses. Although many studies have been performed related to the pathogen virulence and resistance factors, information regarding the host defence mechanisms activated once an infection takes place is still scarce. The present study was designed to understand innate immune responses of farmed juvenile gilthead seabream (Sparus aurata) after Photobacterium damselae subsp. piscicida (Phdp) infection. Therefore, two groups of seabream juveniles were intraperitoneally injected with 100 µL of PBS (placebo) or 100 µL of exponentially growing Phdp (1 × 10⁶ CFU/mL; infected). The blood, plasma, liver, and head kidney of six fish from each treatment were sampled immediately before infection and 3, 6, 9, 24 and 48 h after infection for the broad screening of fish immune and oxidative stress responses. Infected animals presented marked anaemia, neutrophilia and monocytosis, conditions that are correlated with an increased expression of genes related to inflammation and phagocytic activity. Similar studies with different fish species and bacteria can be useful for the definition of health biomarkers that might help fish farmers to prevent the occurrence of such diseases.     

Decision rules for determining terrestrial movement and the consequences for filtering high-resolution global positioning system tracks: a case study using the African lion (Panthera leo)

The combined use of global positioning system (GPS) technology and motion sensors within the discipline of movement ecology has increased over recent years. This is particularly the case for instrumented wildlife, with many studies now opting to record parameters at high (infra-second) sampling frequencies. However, the detail with which GPS loggers can elucidate fine-scale movement depends on the precision and accuracy of fixes, with accuracy being affected by signal reception. We hypothesized that animal behaviour was the main factor affecting fix inaccuracy, with inherent GPS positional noise (jitter) being most apparent during GPS fixes for non-moving locations, thereby producing disproportionate error during rest periods. A movement-verified filtering (MVF) protocol was constructed to compare GPS-derived speed data with dynamic body acceleration, to provide a computationally quick method for identifying genuine travelling movement. This method was tested on 11 free-ranging lions (Panthera leo) fitted with collar-mounted GPS units and tri-axial motion sensors recording at 1 and 40 Hz, respectively. The findings support the hypothesis and show that distance moved estimates were, on average, overestimated by greater than 80% prior to GPS screening. We present the conceptual and mathematical protocols for screening fix inaccuracy within high-resolution GPS datasets and demonstrate the importance that MVF has for avoiding inaccurate and biased estimates of movement.     

Quantification of furanic compounds in coated deep-fried products simulating normal preparation and consumption: Optimisation of HS-SPME analytical conditions by response surface methodology

The validation of a method for the simultaneous quantification of furanic compounds in coated deep-fried samples processed and handled as usually consumed is presented. The deep-fried food was grinded using a device that simulates the mastication, and immediately analysed by headspace solid phase microextraction coupled to gas chromatography-mass spectrometry. Parameters affecting the efficiency of HS-SPME procedure were selected by response surface methodology, using a 2(3) full-factorial central composite design. Optimal conditions were achieved using 2g of sample, 3g of NaCl and 40min of absorption time at 37°C. Consistency between predicted and experimented values was observed and quality parameters of the method were established. As a result, furan, 2-furfural, furfuryl alcohol and 2-pentylfuran were, for the first time, simultaneously detected and quantified (5.59, 0.27, 10.48 and 1.77μgg(-1) sample, respectively) in coated deep-fried fish, contributing to a better understanding of the amounts of these compounds in food.