Measurement of human red blood cell deformability using a single micropore on a thin Si3N4 film

The filtration method for the evaluation of the RBC deformability has been further refined to simulate the deformations encountered in the recticuloendothelial system (in particular the spleen), a recognized site of aged and sickled cells removal. The core of the developed measuring system is a very thin (0.4 micron thick) filter that consists of single micropore (diameters down to 1 micron) on a Si3N4 film which has been constructed using silicon microfabrication techniques. Individual RBC's deformability is quantified measuring the cell pore passage time. From one blood sample 200 passage times are analyzed by a computer, displaying mean and median values as deformability indexes, and class and cumulative histograms for studying the passage times distribution. In this paper the effectiveness of the developed system as a routine clinical evaluation tool is demonstrated by studying several factors that are known to affect the RBC deformability, such as temperature, addition of diamide and glutaraldehyde, and blood storage conditions. In addition, it is experimentally demonstrated that the human RBC can traverse a pore with a diameter as small as 1 micron when the pore length is very short, thus broadening the experimental conditions under which the RBC deformability (fluidity) can be studied.     

A deterministic particle method for the kinetic model of semiconductors: The homogeneous field model

We present a new particle method for the simulation of the semiconductor Boltzmann equation—the weighted particle method. This method differs from the Monte-Carlo method by the approximation of the collision operator—we allocate each particle a weight which varies in time according to the collision integral. This integral is evaluated by means of a quadrature formula, which does not require the use of random numbers. The aim of this paper is to show that this method gives accurate results on physically relevant problems. Linear as well as non-linear collision integrals can be handled the same way by this method. Precise representations of the distribution functions are available, which allow a good insight into the physical processes. In this paper, we only consider the homogeneous field model with an emphasis on the collision operator. Numerical results are presented with a comparison with the Monte-Carlo method.     

The effect of different protein diets on longevity and various biochemical parameters of aged rats]

In this work 23 month old rats were fed for 200 days with different protein diets (NT-diet: 19% protein, 72% of animal origin and LP-diet: 8.8% protein exclusively of vegetable origin). Some metabolic parameters and lifespan (on the base of a 50% death-rate) were determined. The relations of the liver free amino acids glycine + alanine and tyrosine + phenylalanine + branched chain amino acids and the ratio of phenylalanine/tyrosine were determined to be higher in the LP-group. Phenylalanine in liver and urea concentrations in liver and serum were lower in the LP-group. Furthermore the dopamine or serotonin levels were significantly lower in lateral and medial or lateral regions of the hypothalamus respectively in LP-diet fed rats. The norepinephrine content was not modified by the diets. The median lifespan of 23 month old rats was higher by 24% following LP-treatment. These results suggest that the protein component (amino acids) of different diets may modify metabolic parameters and lifespan of animals by mechanisms in which the central regulation may be involved.     

Cimaterol and clenbuterol residue analysis by HPLC-HPTLC in liver.

A method has been developed for the analysis of cimaterol and clenbuterol residues in liver, with detection limits of 0.25 micrograms/kg and 0.5 micrograms/kg, respectively. The recovery varied from 55% to 60%. After extraction, a clean-up procedure with Baker-spe C-18 columns was performed. The two chemical compounds of interest were eluted with methanol. Cimaterol and clenbuterol were quantitatively determined by high-performance liquid chromatography (HPLC) using an RP-Select B (5 microns) column and a post-column reaction procedure. The positive results were confirmed by high-performance thin-layer chromatography (HPTLC) as this technique reaches the same level of sensitivity as the HPLC method.     

Ultrafast all-optical switching in GaAlAs integrated interferometer in 1.55 mu m spectral region

The first observation is reported of ultrafast all-optical switching in an integrated asymmetric Mach-Zehnder interferometer, using the nonresonant nonlinearity in Ga/sub 0.82/Al/sub 0.18/As below half the bandgap. A relative switching fraction of more than 80% has been achieved using 330 fs pulses at around 1.55 mu m from a coupled-cavity mode-locked colour centre laser.<>     

Aging and everyday memory: A cross-cultural study.

Performance on computer-simulated, everyday memory tasks was found to deteriorate with age in 434 Belgian (aged 14–88 yrs) and 434 American Ss matched on gender and age. This age-related memory decline was reasonably consistent across samples. Difficulties in cross-cultural research and the advantages of ecologically valid measurement instruments are discussed. Instruments included a grocery list selective reminding test, the Wechsler Memory Scale, the Benton Visual Retention Test, and the Wechsler Adult Intelligence Scale (WAIS). (PsycINFO Database Record (c) 2010 APA, all rights reserved)     

Improved coupled-mode theory for nonlinear directional couplers

An improved coupled-mode equation for nonlinear directional couplers (NLDC) with Kerr-like nonlinear media is proposed. The method is based on the generalized reciprocity relation in which two sets of field solutions satisfying Maxwell's equations are for the NLDC and for the isolated nonlinear waveguide. That leads to a reasonable result that all the coefficients in the coupled-mode equations, including the coupling coefficient, become power dependent. For the NLDC with a self-focusing or self-defocusing nonlinear material, the authors examine how the coupling coefficient, the coupling length, and the guided power depend on the input power in the range of coupling stronger than in previous reports. It is found that the critical power at which the coupling length becomes infinity does not increase as much with the two guides for the case of self-focusing media     

Uniformly random tree generation

A simple procedure is described for generating a uniformly random tree in a given graph. The probability of obtaining such a tree in a complete graph is given together with the mean number of edge-set selections required and the associated variance.<>     

Evaluating acrosome reaction steps with brightfield and differential interference contrast microscopy techniques.

Acrosomal integrity of bovine spermatozoa was evaluated using naphthol yellow S-erythrosine B stain with bright-field microscopy, .2% glutaraldehyde fixation with Nomarski optics, and live wet smears with Nomarski optics to compare the evaluation techniques. Four ejaculates per two Holstein bulls were enriched through a Percoll gradient, capacitated with heparin, and acrosome-reacted with lysophosphatidylcholine to prepare spermatozoa for in vitro fertilization. Spermatozoa samples were taken after each preparatory step, and percentage of intact acrosomes was evaluated with the stain, .2% glutaraldehyde, and live wet smear. Acrosomal integrity decreased with each preparatory step across all techniques. The decrease in integrity from heparin to lysophosphatidylcholine indicated that all methods detected the acrosome reaction. Technique means across preparation steps showed no differences. Correlations between microscopy techniques were high. The live wet smear used no fixation methods. Both the naphthol yellow S-erythrosine B stain and the .2% glutaraldehyde techniques employed fixation steps that may have introduced artifacts that could influence the data. The live wet smear evaluated by Nomarski optics allowed a comparable evaluation of the acrosome-reacted bovine spermatozoa. Comparing brightfield with differential interference contrast microscopy for detecting the acrosome reaction in bovine spermatozoa showed that both methods were equally accurate.