Volatile compounds of leaves and fruits of Mangifera indica var. coquinho (Anacardiaceae) obtained using solid phase microextraction and hydrodistillation

Volatile compounds present on fruits and leaves of Mangifera indica var. coquinho were investigated by headspace solid phase microextraction (HS-SPME) and hydrodistillation (HD). Conventional techniques, such as hydrodistillation, may impart chemical changes to the original oil composition being also time-consuming. On the other hand, HS-SPME provides solvent-less extractions, shorter extraction times and may supply complementary information about the composition of the compounds. The HS-SPME technique was previously evaluated by the comparative study among the fibres: commercial PDMS, NiTi-ZrO₂ and NiTi-ZrO₂-PDMS. The fibre NiTi-ZrO₂-PDMS showed better sensitivity and precision and was used on the extraction of components. The influence of several parameters like the time and temperature of extraction and desorption time were examined to obtain better efficiency. Fruits and leaves were analysed in mature and immature stages. The profiles corresponding to the volatile compounds detected by both techniques are discussed.     

Comparing the Impact of Environmental Factors During Very High Gravity Brewing Fermentations

The impact of the initial dissolved oxygen, fermentation temperature, wort concentration and yeast pitching rate on the major fermentation process responses were evaluated by full factorial design and statistical analysis by JMP 5.01 (SAS software) software. Fermentation trials were carried out in 2L‐EBC tall tubes using an industrial lager brewing yeast strain. The yeast viability, ethanol production, apparent extract and real degree of fermentation were monitored. The results obtained demonstrate that very high gravity worts at 22°P can be fermented in the same period of time as a 15°P wort, by raising the temperature to 18°C, the oxygen level to about 22 ppm, and increasing the pitching rate to 22 × 10⁶ cell/mL. When diluting to obtain an 11.5°P beer extract, the volumetric brewing capacity increased 91% for the 22°P wort fermentation and 30% using the 15°P wort. After dilution, the fermentation of the 22°P wort resulted in a beer with higher esters levels, primarily the compound ethyl acetate.     

Chitosan biobased and intelligent films: Monitoring pH variations

Many factors can influence the shelf-life product, such as pH, water activity, nutrient levels available oxygen, etc. Variations in food pH could occur due to microorganism growth and chemical reactions that may impact flavour, consistency and shelf-life. The objective of this paper was to obtain and characterize the fast pH-colourimetric indicator device, applying a simple manufacturing technique, using food grade and biodegradable materials. The intelligent film was based on natural compounds as chitosan and anthocyanin (pH-colourimetric indicator). Chitosan intelligent films (C-ATH, 2.0 g/100 g) were obtained incorporating anthocyanin (1.0 g/100 g) in matrix films. Initially, a dark violet colour was observed in chitosan films dried. When immersed in different pH buffers, the colour range of the films varied from pink (in acid pH) to bluish-green (in neutral pH) and to violet (in basic pH). The water solubility and water vapour transmission rate of C-ATH were decreased to 60% and 48%, respectively, as compared to control film (CF, without indicator). The mechanical properties such as tensile strength and rigidity were maintained, and the elongation at break was reduced to 47% compared to CF. The advantages of this system were the simple manufacturing process, biodegradability and usage of natural and safe compounds.     

Nutritional status and its correlates in Equatorial Guinean preschool children: results from a nationally representative survey

BACKGROUND: In Equatorial Guinea, as a result of the recent growth of the oil industry, there is an opportunity to address important public health problems through public and private initiatives. To propose effective nutrition and public health strategies, it is important first to have reliable information on the nutritional status of the population and the underlying factors affecting it. OBJECTIVE: To assess the nutritional status and the prevalence of anemia among Equatoguinean children in a nationally representative sample and to identify the risk factors associated with the nutritional problems detected. METHODS: The study was a cross-sectional survey using a multistaged, stratified, cluster-selected sample. The survey included a sociodemographic, health, and dietary questionnaire and measurement of hematocrit and anthropometric features, from which nutritional indicators based on the National Center for Health Statistics (NCHS) reference and the World Health Organization (WHO) standards were calculated. Logistic regression models were used for the multivariate analysis. A total of 552 children aged 0 to 60 months were surveyed. RESULTS: The overall prevalence of stunting (< -2 height-for-age z-scores [HAZ]) was 29.7% based on the NCHS reference and 35.2% based on WHO standards; the risk factors associated with stunting were age (p < .0001), low socioeconomic status (p = .01), and fishing by a member of the household (p = .003) The prevalence of mild anemia (hemoglobin < 110 g/L) was 69.3%, and that of moderate or severe anemia (hemoglobin < 80 g/L) was 8.3%. The only significant risk factor associated with moderate to severe anemia was low household socioeducational level (p = .01). CONCLUSIONS: Stunting and anemia are public health problems in Equatorial Guinea. Integrated strategies, including fighting poverty and improving maternal education, should be undertaken.     

Sugar Ester Surfactants: Enzymatic Synthesis and Applications in Food Industry

Sugar esters are non-ionic surfactants that can be synthesized in a single enzymatic reaction step using lipases. The stability and efficiency of lipases under unusual conditions and using non-conventional media can be significantly improved through immobilization and protein engineering. Also, the development of de novo enzymes has seen a significant increase lately under the scope of the new field of synthetic biology. Depending on the esterification degree and the nature of fatty acid and/or sugar, a range of sugar esters can be synthesized. Due to their surface activity and emulsifying capacity, sugar esters are promising for applications in food industry.     

Sensory Characterization and Consumer Preference Mapping of Fresh Sausages Manufactured with Goat and Sheep Meat

The main objective of this study was the sensory characterization, by a taste and a consumers’ panel, of fresh sausages from 140 culled goats and 140 culled ewes. Species and type of preparation effects were studied. All data were previously analyzed by analysis of variance. Taste panel data were analyzed by a Generalized Procrustes Analysis (GPA). Consumers’ panel data were analyzed by Preference Mapping. The 1st 2 factors explained 88.22% of total variation in GPA. Different sausages samples were perfectly differentiated by assessors. Goat sausages have been considered harder, more fibrous, and less juicy than sheep sausages. The panelists observed that sheep sausages without paprika had greater intensity of flavor, tasted spicy, and had an off‐odor, while goat sausages with paprika were considered sweeter. Consumers’ panel did not show any preference for the different types of sausages. This means that all types of sausages can have market opportunity.     

Effectiveness of different processing methods in reducing hydrogen cyanide content of flaxseed

A study was conducted to determine the effectiveness of reducing the hydrogen cyanide (HCN) content of flaxseed (FS) by processing. FS was processed by oven heating, single or repeated pelleting alone or in a mix with corn or other ingredients, autoclaving, and microwave roasting. The comparative effectiveness in reducing HCN in FS by these processes was monitored through HCN measurements by alkaline titration. The HCN content was 377 mg kg^?1 in raw feed‐grade FS and 139 mg kg^?1 in a human food‐grade FS. All processing methods tested significantly (p < 0.05) reduced the HCN content of FS. Autoclaving FS reduced its HCN content by 29.7%. Microwave roasting of FS reduced the HCN content by 83.3%. Because of the 5.7% water loss recorded after 4 min of FS roasting, this reduction could be related to more evaporation of the newly formed HCN. Pelleting FS once reduced HCN content by 13.3%, and three and six repeated pelleting processes reduced HCN content by 29.0% and 54.9% respectively. When FS was pelleted in a mix with 50% corn, the HCN reduction was even greater. After pelleting six times, HCN reduction reached 63.8%. However, the greatest reduction in HCN content was 73.8%, and was obtained when FS was mixed with several ingredients and pelleted twice. The HCN reduction could be the result of deactivation of the glycosidase, or the evaporation of HCN formed from cyanogenic glycosides. The HCN reduction increased as the number of pelletings and the temperature of the pelleted product increased. The greater and prolonged exposure to a higher temperature by several pelletings seems to promote a greater HCN reduction. The appropriate processing of FS is essential for the use of this oilseed in animal feeding. Copyright © 2003 Society of Chemical Industry     

A novel humid electronic nose combined with an electronic tongue for assessing deterioration of wine

We report herein the use of a combined system for the analysis of the spoilage of wine when in contact with air. The system consists of a potentiometric electronic tongue and a humid electronic nose. The potentiometric electronic tongue was built with thick-film serigraphic techniques using commercially available resistances and conductors for hybrid electronic circuits; i.e. Ag, Au, Cu, Ru, AgCl, and C. The humid electronic nose was designed in order to detect vapours that emanate from the wine and are apprehended by a moist environment. The humid nose was constructed using a piece of thin cloth sewn, damped with distilled water, forming five hollows of the right size to introduce the electrodes. In this particular case four electrodes were used for the humid electronic nose: a glass electrode, aluminium (Al), graphite and platinum (Pt) wires and an Ag-AgCl reference electrode. The humid electronic nose together with the potentiometric electronic tongue were used for the evaluation of the evolution in the course of time of wine samples. Additionally to the analysis performed by the tongue and nose, the spoilage of the wines was followed via a simple determination of the titratable (total) acidity.     

Increasing selective pressure towards the best compromise in evolutionary multiobjective optimization: The extended NOSGA method

Most current approaches in the evolutionary multiobjective optimization literature concentrate on adapting an evolutionary algorithm to generate an approximation of the Pareto frontier. However, finding this set does not solve the problem. The decision-maker still has to choose the best compromise solution out of that set. Here, we introduce a new characterization of the best compromise solution of a multiobjective optimization problem. By using a relational system of preferences based on a multicriteria decision aid way of thinking, and an outranked-based dominance generalization, we derive some necessary and sufficient conditions which describe satisfactory approximations to the best compromise. Such conditions define a lexicographic minimum of a bi-objective optimization problem, which is a map of the original one. The NOSGA-II method is a NSGA-II inspired efficient way of solving the resulting mapped problem.