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31.
Agata Daszkowska-Golec Edyta Chorazy Miroslaw Maluszynski Iwona Szarejko 《International journal of molecular sciences》2013,14(7):13403-13432
Abscisic acid plays a pivotal role in the abiotic stress response in plants. Although great progress has been achieved explaining the complexity of the stress and ABA signaling cascade, there are still many questions to answer. Mutants are a valuable tool in the identification of new genes or new alleles of already known genes and in elucidating their role in signaling pathways. We applied a suppressor mutation approach in order to find new components of ABA and abiotic stress signaling in Arabidopsis. Using the abh1 (ABA hypersensitive 1) insertional mutant as a parental line for EMS mutagenesis, we selected several mutants with suppressed hypersensitivity to ABA during seed germination. Here, we present the response to ABA and a wide range of abiotic stresses during the seed germination and young seedling development of two suppressor mutants—soa2 (suppressor of abh1 hypersensitivity to ABA 2) and soa3 (suppressor of abh1 hypersensitivity to ABA 3). Generally, both mutants displayed a suppression of the hypersensitivity of abh1 to ABA, NaCl and mannitol during germination. Both mutants showed a higher level of tolerance than Columbia-0 (Col-0—the parental line of abh1) in high concentrations of glucose. Additionally, soa2 exhibited better root growth than Col-0 in the presence of high ABA concentrations. soa2 and soa3 were drought tolerant and both had about 50% fewer stomata per mm2 than the wild-type but the same number as their parental line—abh1. Taking into account that suppressor mutants had the same genetic background as their parental line—abh1, it was necessary to backcross abh1 with Landsberg erecta four times for the map-based cloning approach. Mapping populations, derived from the cross of abh1 in the Landsberg erecta background with each suppressor mutant, were created. Map based cloning in order to identify the suppressor genes is in progress. 相似文献
32.
D. A. Strizhakov V. I. Korbut Kh. M. Kadiev V. E. Agabekov S. N. Khadzhiev 《Petroleum Chemistry》2013,53(4):255-261
Hydrothermal processing of electron-beam irradiated pine sawdust in a mixture with vacuum tower bottoms (VTB) in an autoclave with continuous removal of the vapor phase at a hydrogen pressure of 7.0 MPa has been investigated. It has been found that as the sawdust irradiation dose increases to 1.0 MGy, the degree of VTB conversion during the hydrothermal treatment of the mixture increases and the amount of the hydrocarbon fraction in the resulting liquid product grows to reach a yield of 38.0 wt %. At a temperature of 430°C, the conversion of biomass for two hours is 83–84 wt % and the yields of liquid and gaseous products are 49.0 and 35.0 wt %, respectively. 相似文献
33.
Dorota Pierzchaa Kamila Liput Agnieszka Korwin-Kossakowska Magdalena Oguszka Ewa Poawska Agata Nawrocka Pawe Urbaski Aleksandra Ciepoch Edyta Juszczuk-Kubiak Adam Lepczyski Brygida
laska Krzysztof Kowal Marinus F. W. te Pas Magdalena
miech Pawe Leszczyski Hiroaki Taniguchi Leyland Fraser Przemysaw Sobiech Mateusz Sachajko Magdalena Herudzinska Chandra S. Pareek Mariusz Pierzchaa 《International journal of molecular sciences》2021,22(13)
The molecular mechanism underlying embryonic implantation is vital to understand the correct communications between endometrium and developing conceptus during early stages of pregnancy. This study’s objective was to determine molecular changes in the uterine endometrial proteome during the preimplantation and peri-implantation between 9 days (9D), 12 days (12D), and 16 days (16D) of pregnant Polish Large White (PLW) gilts. 2DE-MALDI-TOF/TOF and ClueGOTM approaches were employed to analyse the biological networks and molecular changes in porcine endometrial proteome during maternal recognition of pregnancy. A total of sixteen differentially expressed proteins (DEPs) were identified using 2-DE gels and MALDI-TOF/TOF mass spectrometry. Comparison between 9D and 12D of pregnancy identified APOA1, CAPZB, LDHB, CCT5, ANXA4, CFB, TTR upregulated DEPs, and ANXA5, SMS downregulated DEPs. Comparison between 9D and 16D of pregnancy identified HP, APOA1, ACTB, CCT5, ANXA4, CFB upregulated DEPs and ANXA5, SMS, LDHB, ACTR3, HP, ENO3, OAT downregulated DEPs. However, a comparison between 12D and 16D of pregnancy identified HP, ACTB upregulated DEPs, and CRYM, ANXA4, ANXA5, CAPZB, LDHB, ACTR3, CCT5, ENO3, OAT, TTR down-regulated DEPs. Outcomes of this study revealed key proteins and their interactions with metabolic pathways involved in the recognition and establishment of early pregnancy in PLW gilts. 相似文献
34.
Joanna Jeruzal-
witecka Edyta Borkowska Mateusz aszczych Zuzanna Nowicka Wioletta Pietruszewska 《International journal of molecular sciences》2022,23(13)
Studies have shown differences in TAS2R38 receptor expression in patients with chronic rhinosinusitis (CRS) compared to healthy controls. Known agonists of TAS2R38 stimulate epithelial cells, leading to robust intracellular nitric oxide (NO) production, which damages bacterial membranes, enzymes, and DNA, but also increases ciliary beat frequency. In this study we examined, using qRT-PCR, the expression of TAS2R38 receptor in nasal polyps (NP) of patients with CRS (N = 107) and in inferior turbinate mucosa (ITM) of patients with CRS and controls (N = 39), and confronted it with clinical features and the severity of the disease. The expression was shown in 43 (50.00%) samples of ITM in the study group (N = 107), in 28 (71.79%) in the control group (N = 39) (p = 0.037), and in 43 (46.24%) of NP. There were no differences in levels of the expression in all analyzed tissues. Patients who rated their symptoms at 0–3 showed higher TAS2R38 expression in ITM in comparison to the patients with 8–10 points on the VAS scale (p = 0.020). A noticeable, however not significant, correlation between the TAS2R38 expression in ITM and the Lund–Mackay CT score was shown (p = 0.068; R = −0.28). Patients with coexisting asthma had significantly higher receptor expression in the NP (p = 0.012). Our study is the first to confirm the presence of the TAS2R38 receptor in NP. Expression of the TAS2R38 receptor is reduced in the sinonasal mucosa in patients with more advanced CRS with NP. 相似文献
35.
Agnieszka Krawczyk-ebek Monika Dymarska Tomasz Janeczko Edyta Kostrzewa-Susow 《International journal of molecular sciences》2022,23(10)
Flavonoid compounds exhibit numerous biological activities and significantly impact human health. The presence of methyl or glucosyl moieties attached to the flavonoid core remarkably modifies their physicochemical properties and improves intestinal absorption. Combined chemical and biotechnological methods can be applied to obtain such derivatives. In the presented study, 4′-methylflavanone was synthesized and biotransformed in the cultures of three strains of entomopathogenic filamentous fungi, i.e., Isaria fumosorosea KCH J2, Beauveria bassiana KCH J1.5, and Isaria farinosa KCH J2.1. The microbial transformation products in the culture of I. fumosorosea KCH J2, flavanone 4′-methylene-O-β-D-(4″-O-methyl)-glucopyranoside, 2-phenyl-(4′-hydroxymethyl)-4-hydroxychromane, and flavanone 4′-carboxylic acid were obtained. Biotransformation of 4′-methylflavanone in the culture of B. bassiana KCH J1.5 resulted in the formation of one main product, i.e., flavanone 4′-methylene-O-β-D-(4″-O-methyl)-glucopyranoside. In the case of I. farinosa KCH J2.6 as a biocatalyst, three products, i.e., flavanone 4′-methylene-O-β-D-(4″-O-methyl)-glucopyranoside, flavanone 4′-carboxylic acid, and 4′-hydroxymethylflavanone 4-O-β-D-(4″-O-methyl)-glucopyranoside were obtained. The Swiss-ADME online simulations confirmed the increase in water solubility of 4′-methylflavanone glycosides and analyses performed using the Way2Drug Pass Online prediction tool indicated that flavanone 4′-methylene-O-β-D-(4″-O-methyl)-glucopyranoside and 4′-hydroxymethylflavanone 4-O-β-D-(4″-O-methyl)-glucopyranoside, which had not been previously reported in the literature, are promising anticarcinogenic, antimicrobial, and hepatoprotective agents. 相似文献
36.
Anna Tylutka Barbara Morawin Edyta Wawrzyniak-Gramacka Eryk Wacka Wiktoria Nowicka Jaroslaw Hiczkiewicz Agnieszka Zembron-Lacny 《International journal of molecular sciences》2022,23(21)
The immunosenescence-related disproportion in T lymphocytes may have important consequences for endothelial dysfunction, which is a key event in vascular aging. The study was designed to assess the prognostic values of the inflammatory-immune profile to better predict and prevent vascular diseases associated with old age. Eighty individuals aged 70.9 ± 5.3 years were allocated to a low- (LGI) or high-grade inflammation (HGI) group based on CRP (<3 or ≥3 mg/L) as a conventional risk marker of cardiovascular diseases. Significant changes in inflammatory and endothelium-specific variables IL-1β, IL-6, TNFα, oxLDL, H2O2, NO, 3-nitrotyrosine, and endothelial progenitor cells (OR 7.61, 95% CI 2.56–29.05, p < 0.0001), confirmed their interplay in vascular inflammation. The flow-cytometry analysis demonstrated a high disproportion in T lymphocytes CD4+ and CD8+ between LGI and HGI groups. CRP was <3 mg/mL for the CD4/CD8 ratio within the reference values ≥ 1 or ≤2.5, unlike for the CD4/CD8 ratio < 1 and >2.5. The odds ratios for the distribution of CD4+ (OR 5.98, 95% CI 0.001–0.008, p < 0.001), CD8+ (OR 0.23, 95% CI 0.08–0.59, p < 0.01), and CD8CD45RO+ T naïve cells (OR 0.27, 95% CI 0.097–0.695, p < 0.01) and CD4/CD8 (OR 5.69, 95% CI 2.07–17.32, p < 0.001) indicated a potential diagnostic value of T lymphocytes for clinical prognosis in aging-related vascular dysfunction. 相似文献
37.
Yu. A. Bashmakov L. A. Gorbov M. S. Korbut A. I. Lebedev L. K. Lytkin S. V. Rusakov 《Instruments and Experimental Techniques》2003,46(4):462-466
The space and energy resolutions of the SPACAL electromagnetic calorimeter with a transverse orientation of the scintillating fibers have been measured. The main parameters of the calorimeter are presented. The results of experiments with an electron test beam of the DESY synchrotron at energies E = 1–6 GeV are discussed. The measured energy resolution of the calorimeter is found to be (/E) = 12.7%/E 2.0% (E is expressed in terms of GeV), and the space resolution is
x, y
(E) = 1 mm at E = 4 GeV. 相似文献
38.
39.
Structural (XRD) and spectroscopic (EPR, IR and Raman) investigations were performed to elucidate the influence of CeO2 content on the phase composition and surface chemistry of CexZr1−xO2 solid solutions (x = 0.10–0.85), interacting with NO and NO2 in the absence and presence of oxygen. Strong influence of ceria loading on the adsorption modes of both nitrogen oxides and the nature of the resultant surface species was revealed. Adsorption of NO led to formation of mononitrosyl complexes, dimers and N2O, whereas interaction of NO2 with the ceria–zirconia catalyst resulted in the adsorbate disproportionation or coupling, depending on the sample composition. 相似文献
40.
The article proposes a new method of obtaining silver nanoparticles on polysiloxane networks using the reducing properties of Si–H groups. Three types of networks with different architecture and distribution of Si–H groups were studied as reducing agents for silver ions and as matrices for the obtained silver nanoparticles. As established by FTIR spectroscopic studies, the redox reaction between Ag+ ions from the silver heptafluorobutyrate solution in toluene and Si–H groups of the networks occurred, which resulted in the appearance of silver nanoparticles in the systems. The amount of metal introduced into individual polysiloxane networks is closely related to the consumption of Si–H groups in them. The type of polysiloxane networks used affects the size of Ag NPs obtained and their distribution on the carrier. Polysiloxane-Ag systems are observed to be an effective catalyst on reduction of hazardous dye like methyl red, which is confirmed by a decrease in absorbance maximum values. 相似文献