Further characterization of the complementation group B temperature-sensitive mutant of respiratory syncytial virus

ts-2, a temperature-sensitive and plaque morphology mutant of respiratory syncytial virus and sole representative of complementation group B, was compared with members of the other complementation groups of respiratory syncytial virus (group A [ts-1] and group C [ts-7]). ts-2 was found to be 10- to 1,000-fold more restricted in growth and ability to spread at restrictive temperatures (37, 38, and 39 degrees C) than at the permissive temperature (32 degrees C). In temperature shift-up experiments, the ts defect of ts-1 and other members of complementation group A was found to effect a late function that was required for at least 13 h in the replicative cycle. The ts lesion of ts-7 affected a function early in the replication cycle. In contrast, ts-2 was not temperature sensitive when studied by the shift-up technique. The discrepancy between the ts plaque property and failure to detect temperature sensitivity during the shift-up experiment was resolved when it was shown that ts-2 had a defect in adsorption or penetration or both at the restrictive temperature. Clonal analysis of revertant ts-2 showed a coordinate restoration of ts+ phenotype ans syncytium-forming capacity. It appears that ts-2 has a defect in a protein that is involved in adsorption and/or penetration of virus and is also responsible for cell fusion activity.     

Synthesis of novel alkyl-aryl polyamide

A laboratory procedure for synthesizing an alkylaryl polyamide curing agent for liquid epoxy resins from commercially available, low cost materials is given. The various chemical reactions required to obtain the desired end-product are described, and several properties of the novel polyamide are listed. This agent was found suitable for producing foamed or frothed epoxy products.     

Crystallinity determination in polymers and a quantitative comparison for polyethylene

A review is given of methods most often used in determining the crystallinity of polymers. In general, emphasis is placed on specific features peculiar to the various techniques rather than experimental aspects. Because of the considerable and increasing use of differential scanning calorimetry, enthalpic methods of crystallinity determination are considered in some detail. The need for a critical selection of parameters representing the fully amorphous and crystalline states is emphasised. This selection can be made for polyethylene, and results of crystallinity determinations using several methods are given for this polymer. Agreement is generally good for unoriented melt-crystallised material (the n.m.r. value is, as expected, anomalous). Interfacial effects are therefore negligible and the structure is adequately represented by a simple two-phase model. Complications arise when polymer is oriented (single crystals grown from solution are included here) and there is little correlation between the various crystallinities.     

The interpretation of impedance measurements on oxide-coated aluminium part 2. The effect of flaws in As-formed films

The interpretation of a.c. impedance data, corrected for solution and double layer losses, is considered for air-formed and anodic alumina films. The effect of flaws on the impedance characteristics could be considerable at the flaw densities observed, and in certain circumstances could predominate over the prevailing structural losses in the bulk film material. A distinction is made between “residual” flaws, associated with regions of impurity segregation in the surface and present in all films, and “mechanical” flaws, associated with relatively gross structural defects in the metal and particularly predominant in air-formed films.     

Defensive sesquiterpenoids from a dipterocarp (Dipterocarpus kerrii)

Four sesquiterpenoids (2, 4, 7, and9) have been isolated and characterized from the termiticidal fraction ofDipterocarpus kerrii resin. The major constituent of this resin is -gurjunene (1).Presented at the Third Annual Meeting of the International Society of Chemical Ecology, June 21–24, 1986, University of California, Berkeley.     

Identifying the task variables that influence perceived object assembly complexity

There is a general lack of understanding as to what issues affect assembly task performance when using diagrammatic instructions because few of the task variables contributing to assembly complexity have been identified. Using a task analysis of a range of self-assembly products, seven task variables hypothesized to predict assembly complexity were identified and studied in the instruction comprehension phase of assembly. Experiment 1 took nine real world assembly instructions and described each in terms of the seven task variables. Seventy-two participants gave a subjective rating of assembly difficulty for each assembly, showing a clear relationship between the task variables and perceived assembly difficulty. As real world assemblies provide little control a second experiment used an orthogonal design to systematically vary the values of each of the assembly task variables in 16 abstract assemblies. Forty-two participants compared the 16 assembly instructions to a final assembly. There was a clear relationship between the task variables and the time taken to view the instructions. Further, it was found that it is possible to predict the complexity of assembly tasks based upon the levels of the task variables identified. The task variables identified are a significant step towards identifying the factors that influence assembly complexity, together with providing progress towards a tool for predicting assembly complexity.     

Spectroelectrochemical sensing based on attenuated total internal reflectance stripping voltammetry. 2. Determination of mercury and lead

Detection of lead and mercury by attenuated total internal reflectance spectroscopy coupled to stripping voltammetry is demonstrated. Changes in attenuation of light passing through an indium tin oxide optically transparent electrode (ITO-OTE) accompany the electrodeposition and stripping of lead and mercury on the electrode surface. The change in absorbance during stripping of electrodeposited metal constitutes the analytical response that enables detection over a range of 2.5 x 10(-7)-5 x 10(-5) and 5 x 10(-8)-5 x 10(-5) M for mercury and lead, respectively. The spectroelectrochemical responses of mercury and lead on the ITO surface are characterized and optimized with respect to solution conditions, the potential excitation signals used for deposition and stripping, and wavelength for detection. The deposited metals were examined by environmental scanning electron miscroscopy, and the electrodeposition pattern of lead and mercury was found to influence the optical response.     

Lysosomes and essential fatty acid deficiency

The hydrolytic activity usually associated with lysosomes increased in the homogenates and subcellular fractions of rat liver as a result of essential fatty acid (EFA) deficiency. The proportion of the total (tissue homogenate) activity found in each subcellular fraction, however, was unchanged by EFA deficiency. Lysosomes isolated from normal and EFA-deficient rat livers differed significantly in their stability to thermal and osmotic variations. This suggested that lysosomal membranes, like other membranes, were altered by EFA deficiency. In spite of increased tissue-bound hydrolytic activity and altered lysosomal membranes, hydrolytic activity of the serum was not markedly changed in EFA deficiency. These minor changes in hydrolytic activity and in lysosomal membrane stability seemed insufficient to explain the general lesions of EFA deficiency. Published with the approval of the Director of The Wisconsin Agricultural Experiment Station.