Deconstructing the Effects of Matrix Elasticity and Geometry in Mesenchymal Stem Cell Lineage Commitment

A wide variety of environmental factors including physical and biochemical signals are responsible for stem cell behavior and function. In particular, matrix elasticity and cell shape have been shown to determine stem cell function, yet little is known about the interplay between how these physical cues control cell differentiation. For the first time, by using ultraviolet (UV) lithography to pattern poly(ethylene) glycol (PEG) hydrogels, it is possible to manufacture microenvironments capable of parsing the effects of matrix elasticity, cell shape, and cell size in order to explore the relationship between matrix elasticity and cell shape in mesenchymal stem cell (MSC) lineage commitment. These data show that cells cultured on 1000 μm² circles, squares, and rectangles are primarily adipogenic lineage regardless of matrix elasticity, while cells cultured on 2500 and 5000 μm² shapes more heavily depend on shape and elasticity for lineage specification. It is further characterized how modifying the cell cytoskeleton through pharmacological inhibitors can modify cell behavior. By showing MSC lineage commitment relationships due to physical signals, this study highlights the importance of cell shape and matrix elasticity in further understanding stem cell behavior for future tissue engineering strategies.     

Design criterion for zero-input asymptotic overflow-stability of recursive digital filters in the presence of quantization

This paper investigates the zero-input stability properties of the exact second-order recursive digital filter having both overflow and quantization non-linearities. Two examples demonstrate the adverse influence of quantization on the overflow-stability property of the filter. Three sets of conditions are presented to ensure asymptotic overflow-stability in the presence of quantization. Using these criteria, various regions in the coefficient plane corresponding to different minimum internal wordlengths required to ensure the non-interaction of overflow and quantization are derived. These results thus form a useful design criterion.This work was supported in part by a Monash Graduate Scholarship (awarded to P.K. Sim) and Telecom Australia under Contract No. 64514.     

Symbolic Compression and Processing of Document Images

In this paper, we describe a compression and representation scheme which exploits the component-level redundancy found within a document image. The approach identifies patterns which appear repeatedly, represents similar patterns with a single prototype, stores the location of pattern instances, and codes the residuals between the prototypes and the pattern instances. Using a novel encoding scheme, we provide a representation that facilitates scalable lossy compression and progressive transmission and supports document image analysis in the compressed domain. We motivate the approach, provide details of the encoding procedures, report compression results, and describe a class of document image understanding tasks that operate on the compressed representation.     

Quantitative automated inspection of standard parts using machine vision

On line automated visual inspection for quality and process control is becoming a very important requirement in an automated manufacturing environment. This paper examines the possibility of real-time inspection of standard part using machine vision.     

Elastic buckling of circular annular plate reinforced with carbon nanotubes

The buckling analysis of circular annular plate reinforced by carbon nanotubes (CNTs) subjected to compressive and torsional loads with various axially symmetric boundary conditions are studied in this paper. The developed model is based on the classical laminated plate theory (CLPT). The Mori‐Tanaka method is employed to calculate the effective elastic modulus of composites having aligned oriented straight CNTs. The eigenvalues of the problem are obtained by means of an analytical approach based on the optimized Rayleigh‐Ritz method. The effects of CNTs orientation angles, edge conditions, geometric ratio of plate and agglomeration of the randomly oriented straight CNTs are investigated on the critical buckling loads. Extensive studies validity of the results based on available data have been carried out prior to the presentation of salient results of this analysis. POLYM. COMPOS., 2011. © 2011 Society of Plastics Engineers     

Finite element modelling the mechanical performance of pressure garments produced from elastic weft knitted fabrics

Compression garments mainly produced from elastic knitted fabrics have attracted many attentions due to their medical care performances. Components’ characteristics of the pressure garments such as yarn and fabric structure affect significantly the pressure applied on the human body. In this paper, it is aimed to simulate the effect of yarn’s mechanical properties as well as fabric structure on mechanical performance of the compression garment. For this purpose, a precise geometrical model for fabric structure is needed by which the pressure applied to the body could be predicted. Accordingly, double jersey knitted fabrics containing elastane weft yarns were produced on an electronic flat knitting machine and the fabric tensile properties were measured in course direction. Using equations governing the fabric structural unit-cell, a real geometric model was created in a finite element software environment. Considering the linear visco-elastic properties for elastane weft yarn, stress-strain curve was extracted. The results obtained from numerical simulation were compared with the experimental data in order to validate the proposed geometrical model. The findings demonstrate a good agreement between experimental and simulation results.     

Physical Analysis of Silver Thin Films Deposited by Electron Beam Technique

Protection of Metals and Physical Chemistry of Surfaces - The effect of annealing temperature on the properties of silver thin films has been investigated. Ag thin films have been deposited on...     

Effect of phytic acid–modified layered double hydroxide on flammability and mechanical properties of intumescent flame retardant polypropylene system

Phytic acid–modified layered double hydroxide (Ph‐LDH) was synthesized via coprecipitation method and subsequently was used in polypropylene (PP) by combining with an ammonium polyphosphate (APP) via melt compounding method. The synergistic effect between APP and Ph‐LDH on the thermal stability, flammability, and mechanical properties of the resultant PP composites was investigated by thermogravimetric analysis, limiting oxygen index, vertical burning test (UL‐94), cone calorimeter tests, tensile test, and impact test. Morphologies of the chars obtained from the samples after the cone calorimeter tests were studied by scanning electron microscopy. The combination of APP and Ph‐LDH slightly influenced the impact and tensile properties of PP. Also, the synergistic effect between APP and Ph‐LDH occurred in the cone calorimeter test. Moreover, the combination of APP and Ph‐LDH produced better quality char that effectively suppressed the spread of the flame and volatile and finally extinguished the fire.     

Improved rheological properties and stability of multiwalled carbon nanotubes/polymer in harsh environment

This work aims to improve the rheological properties and stability of multiwalled carbon nanotubes (MWCNTs)/acrylamide (AA) base skeleton polymer blends at harsh environment of high salinity-high temperature (HS-HT) or various pH. Different co/terpolymers have been accomplished to modify the structure of AA polymer by free-radical copolymerization of AA-based monomers. Anionic, cationic, and hydrophobic functional groups were used for the synthesis of polyelectrolyte, polyampholytic, and partially hydrophobic AA polymer types. The conversion, molecular weight, and poly dispersity of co/terpolymers have been evaluated by nuclear magnetic resonance (¹H-NMR), gel permeation chromatography, and differential scanning calorimetry analysis. The effects of sonication power, concentration of polymer, and concentration of MWCNTs were also investigated on rheological behavior of co/terpolymers. The results show that negative polyelectrolyte and polyampholytic polymers are the best candidates for the improvement of MWCNTs/polymer stability and viscosity at HS-HT and alkali environment, respectively. © 2018 Wiley Periodicals, Inc. J. Appl. Polym. Sci. 2019 , 136, 47205.