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排序方式: 共有144条查询结果,搜索用时 15 毫秒
81.
82.
Proteins in bovine milk are a common source of bioactive peptides. The peptides are released by the digestion of caseins and whey proteins. Peptides derived from the different genetic variants A, B, C, E, F1, F2, G1, G2, H, I, and J of bovine κ-casein (CSN3) were investigated for their inhibitory activities against angiotensin I converting enzyme (ACE). Amino acid sequences of the CSN3 variants were analyzed in silico to detect potential ACE inhibitory peptides. Besides known biologically active peptides, exclusive peptides were identified in some CSN3 variants and their biological activity was determined: within CSN3*B and CSN3*C, the ACE inhibitory peptide ASP (IC50 = 242.3; the IC50 value is equivalent to the micromolar concentration of peptide mediating a 50% inhibition of ACE activity) and within CSN3*C the peptide AHHP (IC50 = 847.6) was detected. Furthermore, the peptides VSP (IC50 = 21.8) and ACHP (IC50 = 360.7) were identified in CSN3*F1 and CSN3*G2, respectively. 相似文献
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85.
Klaus Weimann 《Propellants, Explosives, Pyrotechnics》1993,18(5):294-298
Results of experimental investigations of the influence of charge length and confinement on the velocity, form and terminal ballistic performance of explosively formed projectiles are presented. In order to make quite sure that the projectile shows no asymmetries, a new technique was developed to form projectiles with “star-shaped tail”. 相似文献
86.
Jochen Neuhaus Annett Weimann Mandy Berndt-Paetz 《International journal of molecular sciences》2021,22(21)
The differential activation of Wnt pathways (canonical: Wnt/β-catenin; non-canonical: planar cell polarity (PCP), Wnt/Ca2+) depends on the cell-specific availability and regulation of Wnt receptors, called Frizzled (FZD). FZDs selectively recruit co-receptors to activate various downstream effectors. We established a proximity ligation assay (PLA) for the detection of endogenous FZD–co-receptor interactions and analyzed time-dependent Wnt pathway activation in cultured cells. Prostate cancer cells (PC-3) stimulated by Wnt ligands (Wnt5A, Wnt10B) were analyzed by Cy3-PLA for the co-localization of FZD6 and co-receptors (canonical: LRP6, non-canonical: ROR1) at the single-cell level. Downstream effector activation was assayed by immunocytochemistry. PLA allowed the specific (siRNA-verified) detection of FZD6–LRP6 and FZD6–ROR1 complexes as highly fluorescent spots. Incubation with Wnt10B led to increased FZD6–LRP6 interactions after 2 to 4 min and resulted in nuclear accumulation of β-catenin within 5 min. Wnt5A stimulation resulted in a higher number of FZD6–ROR1 complexes after 2 min. Elevated levels of phosphorylated myosin phosphatase target 1 suggested subsequent Wnt/PCP activation in PC-3. This is the first study demonstrating time-dependent interactions of endogenous Wnt (co-)receptors followed by rapid Wnt/β-catenin and Wnt/PCP activation in PC-3. In conclusion, the PLA could uncover novel signatures of Wnt receptor activation in mammalian cells and may provide new insights into involved signaling routes. 相似文献
87.
Makon R.E. Driad R. Schneider K. Massler H. Aidam R. Quay R. Schlechtweg M. Weimann G. 《Electronics letters》2005,41(17):961-963
A fundamental low phase noise W-band VCO extended by an output buffer using InP/InGaAs DHBT technology is reported. The fully integrated differential VCO exhibits operation frequencies ranging from 83 to 89 GHz. At 87 GHz, a minimum phase noise of -102 dBc/Hz at 1 MHz offset frequency has been achieved. Within the tuning range, a single ended output power up to 5 dBm was measured, resulting in a total signal power of 8 dBm. 相似文献
88.
J. Schicho F.-O. Schreyer M. Weimann 《Applicable Algebra in Engineering, Communication and Computing》2013,24(5):313-341
We develop in this article an algorithm that, given a projective curve $C$ , computes a gonal map, that is, a finite morphism from $C$ to $\mathbb P ^1$ of minimal degree. Our method is based on the computation of scrollar syzygies of canonical curves. We develop an improved version of our algorithm for curves with a unique gonal map and we discuss a characterization of such curves in terms of Betti numbers. Finally, we derive an efficient algorithm for radical parametrization of curves of gonality $\le 4$ . 相似文献
89.
Fillmann G Watson GM Howsam M Francioni E Depledge MH Readman JW 《Environmental science & technology》2004,38(9):2649-2656
While immunoassays have been extensively applied to evaluate environmental contamination, to date they have rarely been used for the analysis of biological fluids outside of human medicine. These media are important because pollutants such as polycyclic aromatic hydrocarbons (PAHs) and their metabolites become concentrated in tissues, body fluids, and excreta, thereby offering a measure of exposure to biologically available contaminants. Such analyses also provide a nondestructive tool for monitoring exposure. Crabs (Carcinus maenas) were exposed to phenanthrene and pyrene (separately) at concentrations ranging from 0 to 200 microg L(-1). After 48 h, urine samples were taken and analyzed by immunoassay and UV-fluorescence spectrophotometry. Urinary levels (calibrated against hydroxylated metabolites) proved to be dose dependent for both compounds, and good agreementwas demonstrated between the immunoassay and the fluorescence techniques. The cross reactivity of the immunochemical technique (ELISA) for pyrene and hydroxy-metabolites was lower than for phenanthrene. HPLC analyses demonstrated that urine from the crabs exposed to pyrene contained mainly conjugate PAH metabolites whose concentrations (the sum of the four main pyrene metabolites/conjugates) showed very good agreement with the ELISA (r2 > 0.94) and fluorescence (r2 > 0.91) data. Environmental samples were also analyzed by ELISA and UV-fluorescence, and both techniques detected PAH (mainly petrogenic) contamination in the urine samples from a polluted harbor. These data demonstrate the potential of urine analyses by ELISA and UV-fluorescence to measure exposure of crabs to PAH. 相似文献
90.
Quantitative trait loci mapping of functional traits in the German Holstein cattle population 总被引:1,自引:0,他引:1
Kühn Ch Bennewitz J Reinsch N Xu N Thomsen H Looft C Brockmann GA Schwerin M Weimann C Hiendleder S Erhardt G Medjugorac I Förster M Brenig B Reinhardt F Reents R Russ I Averdunk G Blümel J Kalm E 《Journal of dairy science》2003,86(1):360-368
A whole-genome scan to detect quantitative trait loci (QTL) for functional traits was performed in the German Holstein cattle population. For this purpose, 263 genetic markers across all autosomes and the pseudoautosomal region of the sex chromosomes were genotyped in 16 granddaughter-design families with 872 sons. The traits investigated were deregressed breedingvalues for maternal and direct effects on dystocia (DYSm, DYSd) and stillbirth (STIm, STId) as well as maternal and paternal effects on nonreturn rates of 90 d (NR90m, NR90p). Furthermore, deregressed breeding values for functional herd life (FHL) and daughter yield deviation for somatic cell count (SCC) were investigated. Weighted multimarker regression analyses across families and permutation tests were applied for the detection of QTL and the calculation of statistical significance. A ten percent genomewise significant QTL was localized for DYSm on chromosome 8 and for SCC on chromosome 18. A further 24 putative QTL exceeding the 5% chromosomewise threshold were detected. On chromosomes 7, 8, 10, 18, and X/Yps, coincidence of QTL for several traits was observed. Our results suggest that loci with influence on udder health may also contribute to genetic variance of longevity. Prior to implementation of these QTL in marker assisted selection programs for functional traits, information about direct and correlated effects of these QTL as well as fine mapping of their chromosomal positions is required. 相似文献