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81.
Capillary zone electrophoresis (CZE) has been applied to separation and characterisation of enzymatic (tryptic) hydrolysates of water-soluble proteins from Bacillus thuringiensis (Bt)-transgenic (Aristis-Bt) and two native non-transgenic (Aristis and Coventry) maize varieties. Water-soluble proteins were extracted from the flour of these maize species and digested by bovine pancreatic trypsin immobilised on agarose gel in 100 mM ammonium hydrocarbonate buffer, pH 7.9. The yielded tryptic digests of proteins were analysed by CZE in four acidic background electrolytes (BGEs) (100 mM H3PO4, 50 mM Tris, pH 2.25; 500 mM acetic acid, pH 2.54; 200 mM formic acid, 200 mM acetic acid, pH 2.05; and 200 mM iminodiacetic acid, pH 2.26) using a lab-made CZE apparatus equipped with bare fused silica capillary and UV-absorption detector operating at 206 nm. Among the tested BGEs, the best resolution of the tryptic peptides of extracted proteins of the above three maize species was obtained in isoelectric BGE, 200 mM iminodiacetic acid, pH 2.26. Selected resolved tryptic peptides of proteins were characterised by effective electrophoretic mobilities and corrected (migration times normalised) peak areas. Some significant relative qualitative and quantitative differences in CZE-UV profiling of tryptic protein digests were found, which can be potentially used to differentiate transgenic Aristis Bt and non-transgenic Aristis varieties or two native non-transgenic varieties, Aristis and Coventry. 相似文献
82.
Natalia D. Lenenko Elena N. Semina Vladimir I. Zaikovskii Alexandre S. Golub 《Materials Chemistry and Physics》2013
Microstructure of layered nanocomposite compound consisting of molybdenum disulfide single layers and the layers of octadecyltrimethylammonium molecules as well as the structure of destruction products of this hybrid compound were studied by high resolution transmission electron microscopy (HRTEM) and powder X-ray diffraction (XRD) techniques. Changes in composition, ordering and morphology of the host and guest layers of the compound occurring due to release of organic guest from the interlayer space of MoS2 on heating or on action of electronic beam have been revealed. Removal of the guest was found to initiate formation in the initial layered structure of the packets consisting of a few MoS2 layers which come close together within the distances of ca. 1–1.5 nm and 0.6–0.7 nm after heating at 250 °C and 400 °C, respectively. Leaving the guest also causes deformations of MoS2 layers resulting in their non-flat geometry. At 400 °C, strong bending of a part of the sulfide layers with the radius as small as 3–4 nm was observed. 相似文献
83.
Guillermo Rodrigo Mark P. Zwart Santiago F. Elena 《Journal of the Royal Society Interface》2014,11(98)
The cornerstone of today''s plant virology consists of deciphering the molecular and mechanistic basis of host–pathogen interactions. Among these interactions, the onset of systemic infection is a fundamental variable in studying both within- and between-host infection dynamics, with implications in epidemiology. Here, we developed a mechanistic model using probabilistic and spatio-temporal concepts to explain dynamic signatures of virus systemic infection. The model dealt with the inherent characteristic of plant viruses to use two different and sequential stages for their within-host propagation: cell-to-cell movement from the initial infected cell and systemic spread by reaching the vascular system. We identified the speed of cell-to-cell movement and the number of primary infection foci in the inoculated leaf as the key factors governing this dynamic process. Our results allowed us to quantitatively understand the timing of the onset of systemic infection, describing this global process as a consequence of local spread of viral populations. Finally, we considered the significance of our predictions for the evolution of plant RNA viruses. 相似文献
84.
Yury I. Lyakhovetsky Elena A. Shilova Boris L. Tumanskii Alexander V. Usatov Era A. Avetisyan Sergei R. Sterlin 《Fullerenes, Nanotubes and Carbon Nanostructures》2013,21(2):263-287
Abstract Both [60]- and [70]fullerene react with either the Scherer radical (perfluorodiisopropylethylmethyl, C9F19), a mixture of branched perfluorononenes (C9F18, the product of hexafluoropropene trimerization), or β-fluorosulphatotetrafluoroethyldiheptafluoroisopropylmethyl radical (C9F18OSO2F) in the ionization chamber of a mass spectrometer to give the positive parent ions of trifluoromethylation products, the reaction being accompanied by hydrogen addition. The reaction occurs at least partly on the walls of the ionization chamber, by a radical mechanism employing CF3 radicals formed from the radical reactants both thermally and under electron impact; only the latter route occurs with the perfluorononenes. 相似文献
85.
86.
Merichel Plaza Susana Santoyo Laura Jaime Belkis Avalo Alejandro Cifuentes Guillermo Reglero Guillermo García-Blairsy Reina Francisco Javier Señoráns Elena Ibáñez 《LWT》2012,46(1):245-253
Chlorella vulgaris has been referred as a potential source of bioactive compounds (carotenoids and fatty acids). In this work, the ability of an environmentally friendly extraction technique such as Pressurized Liquid Extraction (PLE) and a traditional extraction technique such as Ultrasound-Assisted Extraction (UAE) to obtain functional compounds from C. vulgaris, at analytical scale, has been demonstrated. Seeked bioactivities were antioxidant and antimicrobial, for their interest in the food industry. Therefore, a methodology including analytical extraction, in-vitro assays and chemical characterization via HPLC-DAD and GC–MS has been used to determine the interest of Chlorella as a source of functional food ingredients. Results demonstrated that PLE provide higher yields than UAE while similar bioactivities were obtained. Important concentration of carotenoids (lutein, neoxanthin, β-carotene, etc.), chlorophylls, sterols, phytols, and fatty acids (among others) have been found in Chlorella extracts that could be correlated to the observed biological activity. 相似文献
87.
Nikolaos Korfiatis Elena García-Bariocanal Salvador Sánchez-Alonso 《Electronic Commerce Research and Applications》2012,11(3):205-217
Online reviews have received much attention recently in the literature, as their visibility has been proven to play an important role during the purchase process. Furthermore, recent theoretical insight argue that the votes casted on how helpful an online review is (review helpfulness) are of particular importance, since they constitute a focal point for examining consumer decision making during the purchase process. In this paper, we explore the interplay between online review helpfulness, rating score and the qualitative characteristics of the review text as measured by readability tests. We construct a theoretical model based on three elements: conformity, understandability and expressiveness and we investigate the directional relationship between the qualitative characteristics of the review text, review helpfulness and the impact of review helpfulness on the review score. Furthermore, we examine whether this relation holds for extreme and moderate review scores. To validate this model we applied four basic readability measures to a dataset containing 37,221 reviews collected from Amazon UK, in order to determine the relationship between the percentage of helpful votes awarded to a review and the review text’s stylistic elements. We also investigated the interrelationships between extremely helpful and unhelpful reviews, as well as absolutely positive and negative reviews using intergroup comparisons. We found that review readability had a greater effect on the helpfulness ratio of a review than its length; in addition, extremely helpful reviews received a higher score than those considered less helpful. The present study contributes to the ever growing literature on on-line reviews by showing that readability tests demonstrate a directional relationship with average length reviews and their helpfulness and that this relationship holds both for moderate and extreme review scores. 相似文献
88.
Maria Albareda-Sambola Antonio Alonso-Ayuso Laureano F. Escudero Elena Fernández Celeste Pizarro 《Computers & Operations Research》2013
A multi-period discrete facility location problem is introduced for a risk neutral strategy with uncertainty in the costs and some of the requirements along the planning horizon. A compact 0–1 formulation for the Deterministic Equivalent Model of the problem under two alternative strategies for the location decisions is presented. Furthermore, a new algorithmic matheuristic, Fix-and-Relax-Coordination, is introduced. This solution scheme is based on a specialization of the Branch-and-Fix Coordination methodology, which exploits the Nonanticipativity Constraints and uses the Twin Node Family concept. The results of an extensive computational experience allow to compare the alternative modeling strategies and assess the effectiveness of the proposed approach versus the plain use of a state-of-the-art MIP solver. 相似文献
89.
Elena González Antonio Fernández Francesco Bianconi 《Journal of Mathematical Imaging and Vision》2014,50(3):300-313
The use of co-occurrences of patterns in image analysis has been recently suggested as one of the possible strategies to improve on the bag-of-features model. The intrinsically high number of features of the method, however, is a potential limit to its widespread application. Its extension into rotation invariant versions also requires careful consideration. In this paper we present a general, rotation invariant framework for co-occurrences of patterns and investigate possible solutions to the dimensionality problem. Using local binary patterns as bag-of-features model, we experimentally evaluate the potential advantages that co-occurrences can provide in comparison with bag-of-features. The results show that co-occurrences remarkably improve classification accuracy in some datasets, but in others the gain is negligible, or even negative. We found that this surprising outcome has an interesting explanation in terms of the degree of association between pairs of patterns in an image, and, in particular, that the higher the degree of association, the lower the gain provided by co-occurrences in comparison with bag-of-features. 相似文献