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81.
Orlistat is a specific inhibitor of pancreatic and gastric lipases leading to decreased absorption of fat. In the present study, we measured the effect of orlistat on lymphatic fat transport in rats following intake of oils very different in FA composition and TAG structure, and compared this with the transport in normal rats and rats with fat malabsorption. Rats were subjected to cannulation of the main mesenteric lymph duct, and a feeding catheter was inserted into the stomach. In addition, malabsorbing rats were cannulated in the common bile and pancreatic duct. Emulsified safflower, fish, and randomized oils were administered, and lymph was collected for 24 h and analyzed for FA composition. Administration of 25 mg orlistat together with the dietary oils resulted in very small changes from baseline lymphatic transport, indicating that inhibition of the fat absorption was almost complete and furthermore that the source of fat had no influence on the inhibitory effect of orlistat. Orlistat did not interfere with the absorption of the hydrolysis products, since high absorption of sn-2 MAG and FFA (oleic acid) mixed with orlistat was observed. The baseline lymphatic transport in the orlistat group was higher than in the malabsorbing group, but this was the result of generally lower transport of endogenous FA in the malabsorbing group, presumably caused by the absence of bile FA. The transport of FA in normal rats was several-fold higher than the transport after orlistat addition and in malabsorbing rats. Thus, this study showed that orlistat inhibited fat hydrolysis, and thereby lymphatic absorption, almost completely independently of the fat administered. 相似文献
82.
The potential of luminescent semiconductor quantum dots (QDs) to enable development of hybrid inorganic-bioreceptor sensing materials has remained largely unrealized. We report the design, formation and testing of QD-protein assemblies that function as chemical sensors. In these assemblies, multiple copies of Escherichia coli maltose-binding protein (MBP) coordinate to each QD by a C-terminal oligohistidine segment and function as sugar receptors. Sensors are self-assembled in solution in a controllable manner. In one configuration, a beta-cyclodextrin-QSY9 dark quencher conjugate bound in the MBP saccharide binding site results in fluorescence resonance energy-transfer (FRET) quenching of QD photoluminescence. Added maltose displaces the beta-cyclodextrin-QSY9, and QD photoluminescence increases in a systematic manner. A second maltose sensor assembly consists of QDs coupled with Cy3-labelled MBP bound to beta-cyclodextrin-Cy3.5. In this case, the QD donor drives sensor function through a two-step FRET mechanism that overcomes inherent QD donor-acceptor distance limitations. Quantum dot-biomolecule assemblies constructed using these methods may facilitate development of new hybrid sensing materials. 相似文献
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84.
Computer self-efficacy (CSE) is known to enhance individual competence and performance in learning and using technology as well as improve technology attitudes and beliefs. Using longitudinal studies, CSE increases over time during organizational technology training. While these studies have been instrumental in our understanding of how self-efficacy operates in organizations, some critical questions remain unanswered. In particular these studies cannot answer how long it takes for one’s CSE to increase during training, nor can it describe the shape of the change trajectory (linear? some other shape?). The answers to these questions will provide organizations a much clearer perspective on training expectations, understanding when benefits from training through enhanced CSE might be expected, and when to start and ramp up/down training efforts. This study examines these issues by collecting repeated waves of data from 230 respondents in a technology lab training setting and using a relatively new structural equation modeling technique, latent growth modeling. Findings suggest that it takes about 2 months of training for individuals to display significant increases in CSE, and that the growth trajectory for CSE in non-linear. In the proposed model, anxiety is a significant predictor of CSE change, while CSE change significantly predicted software-specific self-efficacies. 相似文献
85.
Psychological health-sickness (PHS) as a predictor of outcomes in dynamic and other psychotherapies.
Luborsky Lester; Diguer Louis; Luborsky Ellen; McLellan A. Thomas; Woody George; Alexander Leslie 《Canadian Metallurgical Quarterly》1993,61(4):542
This is the first dedicated review of quantitative studies on Freud's proposition that the poorer the psychological health, the more limited are the benefits from treatment. Since observer-rated scales for PHS were developed in 1949, many studies have applied them, and the majority show significant prediction of outcomes of psychotherapy, with correlations between .2 and .35. This article reviews (1) the main methods of measurement, (2) the record of predictive success, (3) validity studies, (4) the relation to psychiatric diagnosis, (5) prediction in forms of treatment other than psychotherapy, and (6) theories of why psychological health predicts outcomes of psychotherapy. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
86.
Wax esters (WE) belong to the class of neutral lipids. They are formed by an esterification of a fatty alcohol and an activated fatty acid. Dependent on the chain length and desaturation degree of the fatty acid and the fatty alcohol moiety, WE can have diverse physicochemical properties. WE derived from monounsaturated long-chain acyl moieties are of industrial interest due to their very good lubrication properties. Whereas WE were obtained in the past from spermaceti organs of the sperm whale, industrial WE are nowadays mostly produced chemically from fossil fuels. In order to produce WE more sustainably, attempts to produce industrial WE in transgenic plants are steadily increasing. To achieve this, different combinations of WE producing enzymes are expressed in developing Arabidopsis thaliana or Camelina sativa seeds. Here we report the identification and characterization of a fifth wax synthase from the organism Marinobacter aquaeolei VT8, MaWSD5. It belongs to the class of bifunctional wax synthase/acyl-CoA:diacylglycerol O-acyltransferases (WSD). The protein was purified to homogeneity. In vivo and in vitro substrate analyses revealed that MaWSD5 is able to synthesize WE but no triacylglycerols. The protein produces WE from saturated and monounsaturated mid- and long-chain substrates. Arabidopsis thaliana seeds expressing a fatty acid reductase from Marinobacter aquaeolei VT8 and MaWSD5 produce WE. Main WE synthesized are 20:1/18:1 and 20:1/20:1. This makes MaWSD5 a suitable candidate for industrial WE production in planta. 相似文献
87.
88.
A social-cognitive approach to motivation and personality. 总被引:9,自引:0,他引:9
Past work has documented and described major patterns of adaptive and maladaptive behavior: the mastery-oriented and the helpless patterns. In this article, we present a research-based model that accounts for these patterns in terms of underlying psychological processes. The model specifies how individuals' implicit theories orient them toward particular goals and how these goals set up the different patterns. Indeed, we show how each feature (cognitive, affective, and behavioral) of the adaptive and maladaptive patterns can be seen to follow directly from different goals. We then examine the generality of the model and use it to illuminate phenomena in a wide variety of domains. Finally, we place the model in its broadest context and examine its implications for our understanding of motivational and personality processes. (PsycINFO Database Record (c) 2010 APA, all rights reserved) 相似文献
89.
90.
Transreac is a computer model for the simulation of the chemical attack to which mineral building materials are exposed. For reliability analysis, Transreac is used in conjunction with the Monte Carlo simulation method. The probabilistic model was evaluated for cement mortar corroded as a result of sulfate attack. The experimentally derived corrosion effects and their scatter are in good agreement with simulation results. In addition, the probability of failure was predicted and a sensitivity analysis was performed. 相似文献