Therapeutic Effects of hiPSC-Derived Glial and Neuronal Progenitor Cells-Conditioned Medium in Experimental Ischemic Stroke in Rats

Transplantation of various types of stem cells as a possible therapy for stroke has been tested for years, and the results are promising. Recent investigations have shown that the administration of the conditioned media obtained after stem cell cultivation can also be effective in the therapy of the central nervous system pathology (hypothesis of their paracrine action). The aim of this study was to evaluate the therapeutic effects of the conditioned medium of hiPSC-derived glial and neuronal progenitor cells in the rat middle cerebral artery occlusion model of the ischemic stroke. Secretory activity of the cultured neuronal and glial progenitor cells was evaluated by proteomic and immunosorbent-based approaches. Therapeutic effects were assessed by overall survival, neurologic deficit and infarct volume dynamics, as well as by the end-point values of the apoptosis- and inflammation-related gene expression levels, the extent of microglia/macrophage infiltration and the numbers of formed blood vessels in the affected area of the brain. As a result, 31% of the protein species discovered in glial progenitor cells-conditioned medium and 45% in neuronal progenitor cells-conditioned medium were cell type specific. The glial progenitor cell-conditioned media showed a higher content of neurotrophins (BDNF, GDNF, CNTF and NGF). We showed that intra-arterial administration of glial progenitor cells-conditioned medium promoted a faster decrease in neurological deficit compared to the control group, reduced microglia/macrophage infiltration, reduced expression of pro-apoptotic gene Bax and pro-inflammatory cytokine gene Tnf, increased expression of anti-inflammatory cytokine genes (Il4, Il10, Il13) and promoted the formation of blood vessels within the damaged area. None of these effects were exerted by the neuronal progenitor cell-conditioned media. The results indicate pronounced cytoprotective, anti-inflammatory and angiogenic properties of soluble factors secreted by glial progenitor cells.     

Effect of HCl pre-treatment on corrosion resistance of cerium-based conversion coatings on magnesium and magnesium alloys

The corrosion protection afforded by a cerium conversion coating, formed by immersion in a solution containing rare earth salt and hydrogen peroxide, on pure magnesium and two magnesium alloys, AZ91 and AM50, has been studied. The effect of HCl pre-treatments on the morphology and on the corrosion resistance of the cerium conversion layer was investigated. A thicker and more homogeneous distribution of the conversion coating was obtained when the sample surface was pre-treated with acid. Higher amounts of cerium on the surface of the pre-treated samples were detected. The cerium conversion coating increased the corrosion resistance of the alloys because it ennobled the corrosion potential and decreased both the anodic and cathodic current. The acid pre-treatment further increased the corrosion resistance of the coated alloys. After five days of immersion in chloride environment the untreated samples showed localized corrosion while the chemical conversion coated samples appeared unaffected.     

Synthesis and characterization of polyurea microcapsules containing essential oils with antigerminative activity

In this paper we report on the preparation and characterization of polyurea‐based microencapsulated systems, containing essential oils as core materials, for potential applications in controlled‐release formulations of agrochemicals. Microcapsules were synthesized by interfacial polymerization in o/w emulsion between polyfunctional isocyanates and diamines, to investigate the effect of the monomer kind on the morphology and properties of the produced samples. The synthetic conditions that gave the best results were used to microencapsulate four essential oils, able to interfere with the seed germination and radicle elongation of some test plants. The produced samples were characterized, with the aim to analyze their morphology and to verify the effectiveness of essential oil microencapsulation. Moreover, preliminary bioassay based on seed germination and subsequent radical growth were carried out to study the effects of the microencapsulated essential oils. © 2007 Wiley Periodicals, Inc. J Appl Polym Sci 2007     

追求影像臻于完美

Nick Lee澳籍华人,有十余年的广告创意经验,曾担任过跨国传媒广告机构的创意总监等工作,同时为多家中国企业客户和众多跨国企业提供过成功的服务及案例.现创立一支精锐的团队REDFLAG STUDIO(商艺广告传播)于上海,有志借助这个平台,不仅为客户提供高品质的实视化作品,而且希望能够为中国的"创意视觉经济"的发展助力.     

Die design for rigid PVC—the effect of die land length on extrudate swell

PVC profile extrusion compounds have a unique morphology. While other polymers gradually decrease in extrusion die swell with increasing length/thickness (L/D) ratio, PVC profile extrusion compounds have a low die swell, quite independent of the die's L/D ratio in the range of 5 to 20. The fact that the die land length can be changed without changing the extrudate swell is an important consideration, which makes die design and balancing dies simpler and easier for PVC profile extrusion compounds. While other polymers substantially increase extrudate swell with increased shear rate, the swell of the PVC profile compounds is not much affected by shear or extrusion rate. This unique behavior allows wider processing latitude in profile extrusion and faster extrusion rates than with other polymers. Another unique factor in the rheology of PVC profile extrusion compounds is that extrusion die swell increases with increasing melt temperature, while other polymers have decreasing die swell with increasing melt temperature. The unusual rheology of PVC profile extrusion compounds is attributed to its unique melt morphology, where the melt flow units are 1 um bundles and molecules that have low surface to surface interaction and entanglement at low processing temperatures but increased melting and increased entanglement at higher processing temperatures. Other polymers, unlike PVC, have melt flow at the molecular level.     

A coarse-grain reconfigurable architecture for multimedia applications supporting subword and floating-point calculations

Signal processors exploiting ASIC acceleration suffer from sky-rocketing manufacturing costs and long design cycles. FPGA-based systems provide a programmable alternative for exploiting computation parallelism, but the flexibility they provide is not as high as in processor-oriented architectures: HDL or C-to-HDL flows still require specific expertise and a hardware knowledge background. On the other hand, the large size of the configuration bitstream and the inherent complexity of FPGA devices make their dynamic reconfiguration not a very viable approach. Coarse-grained reconfigurable architectures (CGRAs) are an appealing solution but they pose implementation problems and tend to be application specific. This paper presents a scalable CGRA which eases the implementation of algorithms on field programmable gate array (FPGA) platforms. This design option is based on two levels of programmability: it takes advantage of performance and reliability provided by state-of-the-art FPGA technology, and at the same time it provides the user with flexibility, performance and ease of reconfiguration typical of standard CGRAs. The basic cell template provides advanced features such as sub-word SIMD integer and floating-point computation capabilities, as well as saturating arithmetic. Multiple reconfiguration contexts and partial run-time reconfiguration capabilities are provided, tackling this way the problem of high reconfiguration overhead typical of FPGAs. Selected instances of the proposed architecture have been implemented on an Altera Stratix II EP2S180 FPGA. On this system, we mapped some common DSP, image processing, 3D graphics and audio compression algorithms in order to validate our approach and to demonstrate its effectiveness by benchmarking the benefits achieved.     

A coarse-grain reconfigurable architecture for multimedia applications featuring subword computation capabilities

This paper presents the design and the implementation of a coarse-grain reconfigurable machine used as an accelerator for a programmable RISC core, to speed up the execution of computationally demanding tasks like multimedia applications. We created a VHDL model of the proposed architecture and implemented it on a FPGA board for prototyping purposes; then we mapped on our architecture some DSP and image processing algorithms as a benchmark. In particular, we provided the proposed architecture with subword computation capabilities, which turns out to be extremely effective especially when dealing with image processing algorithms, achieving significant benefits in terms of speed and efficiency in resource usage. To create the configuration bitstream (configware) we created a tool based on a graphical user interface (GUI) which provides a first step towards the automation of the programming flow of our design: the tool is meant to ease the life of the programmer, relieving him from the burden of calculating the configuration bits by hand. Synthesis results indicate that the area occupation and the operating frequency of our design are reasonable also when compared to other similar design. In addition to this, the amount of clock cycles taken by our machine to perform a given algorithm is orders of magnitude smaller than the one required by a corresponding software implementation on a RISC microprocessor.     

Real-time scheduling for energy harvesting sensor nodes

Energy harvesting has recently emerged as a feasible option to increase the operating time of sensor networks. If each node of the network, however, is powered by a fluctuating energy source, common power management solutions have to be reconceived. This holds in particular if real-time responsiveness of a given application has to be guaranteed. Task scheduling at the single nodes should account for the properties of the energy source, capacity of the energy storage as well as deadlines of the single tasks. We show that conventional scheduling algorithms (like e.g. EDF) are not suitable for this scenario. Based on this motivation, we have constructed optimal scheduling algorithms that jointly handle constraints from both energy and time domain. Further we present an admittance test that decides for arbitrary task sets, whether they can be scheduled without deadline violations. To this end, we introduce the concept of energy variability characterization curves (EVCC) which nicely captures the dynamics of various energy sources. Simulation results show that our algorithms allow significant reductions of the battery size compared to Earliest Deadline First scheduling.

Maternally modulated infant separation responses are regulated by D2-family dopamine receptors.

Although dopamine is necessary for mammalian adult pair-bond formation and maternal behavior, its function in infant social behavior and attachment has been less thoroughly explored. The vocalization rate of an isolated rat pup is influenced by recent social contact. Interactions with the dam potentiate vocalization rate. Interactions with littermates or adult males do not. Systemic administration of the D2-family agonist quinpirole specifically blocked maternal potentiation at doses that did not alter vocalization rate in an isolation prior to dam contact. This result was not explained by quinpirole's effects on body temperature or locomotion. The results are consistent with a role for dopamine in infant social behavior. (PsycINFO Database Record (c) 2010 APA, all rights reserved)