A 5‐MHz AT‐cut quartz crystal oscillating circuit functioning in 350 nA

An oscillating circuit functioning at ultra low power (350 nA) for a 5‐MHz AT‐cut quartz crystal oscillator was investigated. This circuit has a resistance between the power terminal of the CMOS‐IC and the power supply, and another between the earth terminal of the CMOS‐IC and the ground (GND). These resistances discourage an inrush of current, and set a gain (g_m) necessary for oscillating the circuit at minimum. The developed circuit is quite simple, but enables driving at once‐unthinkable, low power (below 1 µA). © 2007 Institute of Electrical Engineers of Japan. Published by John Wiley & Sons, Inc.     

Electrochemistry of LiMn2O4 epitaxial films deposited on various single crystal substrates

LiMn₂O₄ epitaxial thin films were synthesized on SrTiO₃:Nb(1 1 1) and Al₂O₃(0 0 1) single crystal substrates by pulsed laser deposition (PLD) method and the electrochemical properties were discussed comparing with that of amorphous LiMn₂O₄ film on polycrystalline Au substrate. LiMn₂O₄ epitaxial film showed only a single plateau in charge–discharge curves and a single redox peak at the corresponding voltage of cyclic voltammograms. This phenomenon seems to originate from the effect of the epitaxy: the film is directly connected with the substrate by the chemical bond and this connection would suppress the phase transition of Li_xMn₂O₄ film during lithium (de-)intercalation. The discharge voltage of LiMn₂O₄ epitaxial film on SrTiO₃ was lower than that of LiMn₂O₄ film on Al₂O₃. This lowered discharge voltage may be caused by the electronic interaction between LiMn₂O₄ film and SrTiO₃:Nb n-type semiconductor substrate.     

Determination of epoxidized soybean oil and linseed oil in wrapping film and cap sealing

A determination method was developed for epoxidized soybean oil (ESBO) and epoxidized linseed oil (ELO), which are used as plasticizers and/or stabilizers, in wrapping film and cap sealings. The ESBO method reported by Castle et al. was improved. Samples were extracted with acetone-hexane (3: 7), transmethylated under alkaline conditions, then derivatized to the 1,3-dioxolanes and analyzed by GC/MS. The recoveries of spiked ESBO and ELO were between 92.6% and 104.4%. The determination limits were 0.01 mg/g for ESBO and 0.02 mg/g for ELO in the wrapping film, and 0.04 mg/g and 0.08 mg/g in the cap sealing. ESBO and ELO were surveyed in 10 samples each of wrapping film and cap sealings currently available on the Japanese market. ESBO was found at 34.7-82.8 mg/g in polyvinyl chloride (PVC) wrapping films and at 5.47-399 mg/g in cap sealings. ELO was detected at 8.6-11.4 mg/g in polyvinylidene chloride (PVDC) wrapping films, and at 46.4 mg/g in a PVC wrapping film.     

TWO REACTION PATH KINETICS IN CO OXIDATION ON ZINC OXIDE

The mode of transient response curves in CO oxidation on ZnO is drastically changed to a false start, an overshoot or an instantaneous mode, depending on the concentration region of CO. These characteristic modes and the anomalous steady state rate data which show maximum and minimum are consistently explained by the two reaction pathways model.     

Molecular analysis of glucose phosphate isomerase deficiency associated with hereditary hemolytic anemia

We report here two new cases of glucose phosphate isomerase (GPI) deficiency associated with hemolytic anemia and present the results of molecular analysis of the five Japanese GPI variants. A Japanese girl (GPI Fukuoka) had an episode of prolonged neonatal jaundice and at 3 years of age was admitted due to acute hemolytic crisis occurring with upper respiratory tract infection. Red blood cell (RBC) GPI activity was decreased to 11.8% of normal and the reduced glutathione (GSH) level of RBCs was slightly decreased. A 54-year-old Japanese man (GPI Iwate) was hospitalized due to chronic active hepatitis, and compensated hemolysis was noted. RBC GPI activity of the proband was decreased to 18.8%, and the GSH content was about half of the normal mean value. Sequencing of the reticulocyte GPIcDNA showed homozygous missense mutations 1028CAG-->CGG (343Gln-->Arg), 14ACC-->A7C (5Thr-->lle), 671ACG-->A7G (224Thr-->Met), and 1615GAC-->AAC (539Asp-->Asn) in GPI Narita, GPI Matsumoto, GPI Iwate, and GPI Fukuoka, respectively. We also identified GPI Kinki as a compound heterozygote of 1124ACA-->AGA(375Thr-->Arg)/ 1615GAC-->AAC(539Asp-->Asn). Our findings, together with the previous results of other investigators, showed that the GPI gene mutations so far identified were heterogeneous, although most GPI variants had common biochemical characteristics such as heat instability and normal kinetics. Several amino acid substitutions were identified in the proximity of the catalytically important amino acid residues such as Ser/Asp 159/160, Asp341, and Lys518, which have been identified in the structural analysis of the pig GPI. The molecular characterization of human GPI variants, therefore, may provide new insights into the genotype-phenotype correlation of GPI deficiency as well as the structure-function relationship of this enzyme.     

Super catalytic antibody and antigenase

By immunizing ground-state peptides or proteins, we can produce super catalytic antibodies possessing serine protease-like characteristics. The unique feature of super catalytic antibodies is their ability to decompose a target molecule that is being killed. The authors have succeeded in preparing super catalytic antibodies that destroy (i) the HIV-1 envelope protein gp41, (ii) chemokine receptor CCR5 peptide, and (iii) Helicobacter pylori urease, etc. Some of them can degrade antigens at high catalytic reaction rates. Regarding their Km and kcat, super catalytic antibodies show intermediary values between that of enzymes (high Km and kcat) and that of antibodies (low Km and kcat [=0]). The catalytic function of an antibody mostly resides in its light chain. From mouse Vkappa germline analysis, it became clear that super catalytic antibodies are generated from some discrete germlines such as bb1, cr1, cs1, bl1, bj2 and bd2. In these Vkappa germlines, at least one catalytic triad composed of three amino acid residues, namely, Asp1, Ser27a and His93, is encoded. Namely, the antibody light chains (super catalytic antibodies) generated from the germlines are inherently able to enzymatically decompose antigens. Thus, such antibody light chains can be referred to as antigenase (antigen-decomposing enzyme) and may have arisen during the evolution of antibodies to acquire a higher ability than that of enzymes for developing a sophisticated self-defense system for survival.