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41.
42.
This review describes the current knowledge and applications of pulsatile flow in microfluidic systems. Elements of fluid dynamics at low Reynolds number are first described in the context of pulsatile flow. Then the practical applications in microfluidic processes are presented: the methods to generate a pulsatile flow, the generation of emulsion droplets through harmonic flow rate perturbation, the applications in mixing and particle separation, and the benefits of pulsatile flow for clog mitigation. The second part of the review is devoted to pulsatile flow in biological applications. Pulsatile flows can be used for mimicking physiological systems, to alter or enhance cell cultures, and for bioassay automation. Pulsatile flows offer unique advantages over a steady flow, especially in microfluidic systems, but also require some new physical insights and more rigorous investigation to fully benefit future applications. 相似文献
43.
Layered Simple Hydroxides Functionalized by Fluorene‐Phosphonic Acids: Synthesis,Interface Theoretical Insights,and Magnetoelectric Effect
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Quentin Evrard Ziyad Chaker Mélissa Roger Charlotte M. Sevrain Emilie Delahaye Mathieu Gallart Pierre Gilliot Cédric Leuvrey Jean‐Michel Rueff Pierre Rabu Carlo Massobrio Mauro Boero Alain Pautrat Paul‐Alain Jaffrès Guido Ori Guillaume Rogez 《Advanced functional materials》2017,27(41)
Copper‐ and cobalt‐based layered simple hydroxides (LSH) are successfully functionalized by a series of fluorene mono‐ and diphosphonic acids, using anionic exchange reactions and a preintercalation strategy. The lateral functionalization of the fluorene moieties has only little impact on the overall structure of the obtained layered hybrid materials but it influences the organization of the molecules within the interlamellar spacing. For bulky fluorene (9,9‐dioctyl derivative), luminescence is preserved when inserted into copper and cobalt hydroxydes, whereas it is completely quenched for the other fluorenes. Detailed characterization of the internal structure and chemical bonding properties for copper‐ and cobalt‐based hybrids is performed via ancillary experimental techniques. For the copper‐based LSH class, for which more elusive findings are found, first‐principles molecular dynamics simulations unravel the fundamental stabilizing role of the H‐bonding network promoted within the local environments of the fluorene mono‐ and diphosphonic acids. The cobalt series of compounds constitute a new class of hybrid magnets, with ordering temperatures ranging from 11.8 to 17.8 K and show a clear magnetoelectric effect. This effect appears above a threshold magnetic field, which is null below the magnetic ordering temperature, and it persists in the paramagnetic regime till about 110 K. 相似文献
44.
Analysis of bacterial diversity in river biofilms using 16S rDNA PCR-DGGE: methodological settings and fingerprints interpretation 总被引:5,自引:0,他引:5
Reliability of bacterial diversity assessment using polymerase chain reaction (PCR) denaturing gradient gel electrophoresis (DGGE) analysis of 16S rDNA fragments was evaluated for a particular complex microbial assemblage: river epilithic biofilm. By comparing 3 routine protocols on replicates of one river biofilm sample, we found that common DNA extraction procedures gave comparable diversity (from 28.0 to 30.7 bands detected) and community composition (> 75% of homology) despite differences in the total amount of extracted DNA (from 0.9 to 4.2 microg). Therefore methodological improvements only concerned electrophoretic separation of DNA fragments (range of denaturing gradient from 35% to 70% and migration time=18h) and standardisation of DNA amounts used (PCR-template=50 ng, gel loading=700 ng). Using such a standardised methodology we found a good reproducibility of all steps of the procedure. When an Escherichia coli strain was introduced as a contaminant in a biofilm sample, we were able to recover ribotypes from the strain. As concerns fields sampling, a satisfactory repeatability of banding patterns from neighbouring pebbles (sampling point) allowed discriminating between the biofilm intrasite variability (various points from a cross-profile). These trials confirmed that PCR-DGGE is suitable to assess a reliable genetic fingerprint of epilithic biofilms in the river. Phylogenetic analysis of 40 partial sequences of 16S rDNA from DGGE gels of two sets of river biofilms samples proved evidences for the retrieval of DNA fragments related to phototroph Eukarya. However, in both cases plastidial 16S rDNA represented less than 25% of the analysed operational taxonomic units. Taking into account that Cyanobacteria, as members of the Bacteria, were also detected, sequence analysis of relevant bands from the pattern is required to target "bacteria", i.e. the functional group of prokaryotic microorganisms to which one commonly refers as a key component in sustaining the nutrient turnover. 相似文献
45.
Bussy C Cambedouzou J Lanone S Leccia E Heresanu V Pinault M Mayne-L'hermite M Brun N Mory C Cotte M Doucet J Boczkowski J Launois P 《Nano letters》2008,8(9):2659-2663
X-ray fluorescence microscopy (microXRF) is applied for the first time to study macrophages exposed to unpurified and purified single-walled (SW) and multiwalled (MW) carbon nanotubes (CNT). Investigating chemical elemental distributions allows one to (i) image nanotube localization within a cell and (ii) detect chemical modification of the cell after CNT internalization. An excess of calcium is detected for cells exposed to unpurified SWCNT and MWCNT and related toxicological assays are discussed. 相似文献
46.
The ductility of an elastic structure with a growing crack may be defined as the ratio of the additional load-point displacement that is caused by the crack at the moment of loss of stability under displacement control to the elastic displacement at no crack at the moment of peak load. The stability loss at displacement control is known to occur when the load-deflection curve of the whole structural system with the loading device (characterized by a spring) reaches a snapback point. Based on the known stress intensity factor as a function of crack length, the well-known method of linear elastic fracture mechanics is used to calculate the load-deflection curve and determine the states of snapback and maximum loads. An example of a notched three-point bend beam with a growing crack is analyzed numerically. The ductility is determined and its dependence of the structure size, slenderness, and stiffness of the loading device is clarified. The family of the curves of ductility versus structure size at various loading device stiffnesses is found to exhibit at a certain critical stiffness a transition from bounded single-valued functions of D to unbounded two-valued functions of D. The method of solution is general and is applicable to cracked structures of any shape. The flexibility (force) method can be adapted to extend the ductility analysis to structural assemblages provided that the stress intensity factor of the cracked structural part considered alone is known. This study leads to an improved understanding of ductility, which should be useful mainly for design against dynamic loads. 相似文献
47.
A Grubisic E Ringe CM Cobley Y Xia LD Marks RP Van Duyne DJ Nesbitt 《Nano letters》2012,12(9):4823-4829
Electron emission from single, supported Ag nanocubes excited with ultrafast laser pulses (λ = 800 nm) is studied via spatial and polarization correlated (i) dark field scattering microscopy (DFM), (ii) scanning photoionization microscopy (SPIM), and (iii) high-resolution transmission electron microscopy (HRTEM). Laser-induced electron emission is found to peak for laser polarization aligned with cube diagonals, suggesting the critical influence of plasmonic near-field enhancement of the incident electric field on the overall electron yield. For laser pulses with photon energy below the metal work function, coherent multiphoton photoelectron emission (MPPE) is identified as the most probable mechanism responsible for electron emission from Ag nanocubes and likely metal nanoparticles/surfaces in general. 相似文献
48.
49.
Modulation of Starch Digestibility in Breakfast Cereals Consumed by Subjects with Metabolic Risk: Impact on Markers of Oxidative Stress and Inflammation during Fasting and the Postprandial Period
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Stéphanie Lambert‐Porcheron Sylvie Normand Emilie Blond Monique Sothier Hubert Roth Alexandra Meynier Sophie Vinoy Martine Laville Julie‐Anne Nazare 《Molecular nutrition & food research》2017,61(12)
Scope : Decreasing postprandial glycaemic excursions may have a beneficial effect on inflammatory and oxidative stress profiles. In this study, we investigated the impact of carbohydrate digestibility modulation per se, as a means of reducing the glycaemic response, on metabolic and inflammatory responses in subjects with metabolic risk factors. Methods and results : Twenty healthy subjects with metabolic risk consumed a cereal product either high in Slowly Digestible Starch (HSDS) or low in SDS (LSDS) at breakfast daily for 3 weeks, in a cross‐over design. Following each 3‐week session, postprandial glycaemia, insulinaemia, the lipid profile, inflammation and oxidative stress markers were assessed and compared to those induced by ingestion of a glucose solution (as a reference). The 2‐h glycaemic and insulinaemic responses were significantly lower following the HSDS breakfast compared with the LSDS breakfast or glucose. No significant differences between the products were observed in terms of the lipid profile, C‐reactive protein, IL‐6 and tumour necrosis factor alpha. We observed a slight increase in fasting lipid peroxidation markers, including an increase in plasma malondialdehyde (MDA) and a decrease in whole blood glutathione (GSH), without significant alteration of urinary F2‐isoprostanes or plasma glutathione peroxidase (GPx) activity. Conclusion : Consumption of HSDS products for 3 weeks significantly altered both postprandial glycaemia and insulinaemia, but was not sufficient to modify the inflammatory profile. Consumption of both cereal products was associated with a slightly higher fasting oxidative stress profile. 相似文献
50.
Fructose overfeeding in first‐degree relatives of type 2 diabetic patients impacts energy metabolism and mitochondrial functions in skeletal muscle
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