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排序方式: 共有1731条查询结果,搜索用时 15 毫秒
61.
Del Moral ML Esteban FJ Hernández R Blanco S Molina FJ Martínez-Lara E Siles E Viedma G Ruiz A Pedrosa JA Peinado MA 《Microscopy research and technique》2004,64(4):304-311
To ascertain the possible implications of the nitric oxide (NO*) producing system in striatal senescence, and by using immunohistochemistry and image-processing approaches, we describe the presence of the enzyme nitric oxide synthase (NOS), the NADPH-diaphorase (NADPH-d) histochemical marker, and nitrotyrosine-derived complexes (N-Tyr) in the striatum of adult and aged rats. The results showed neuronal NOS immunoreactive (nNOS-IR) aspiny medium-sized neurons and nervous fibres in both age groups, with no variation in the percentage of immunoreactive area but a significant decrease in the intensity and in the number of somata with age, which were not related to the observed increase with age of the striatal bundles of the white matter. In addition, NADPH-d activity was detected in neurons with morphology similar to that of the nNOS-IR cells; a decrease in the percentage of area per field and in the number of cells, but an increase in the intensity of staining for the NADPH-d histochemical marker, were detected with age. The number of neuronal NADPH-d somata was higher than for the nNOS-IR ones in both age groups. Moreover, N-Tyr-IR complexes were observed in cells (neurons and glia) and fibres, with a significant increase in the percentage of the area of immunoreaction, related to the increase of white matter, but a decrease in intensity for the aged group. On the other hand, we did not detect the inducible isoform (iNOS) either in adult or in aged rats. Taken together, these results support the contention that NADPH-d staining is not such an unambiguous marker for nNOS, and that increased protein nitration may participate in striatal aging. 相似文献
62.
Roberto Moreno Soriano Nuria Rojas Emilio Nieto Rosa de Guadalupe González-Huerta Juan Manuel Sandoval-Pineda 《International Journal of Hydrogen Energy》2021,46(51):25944-25953
To keep optimally connected, all electrolysis cell elements is one of the most important design criteria. The optimal distribution of the clamping points is crucial to increasing cell performance. In this work, the compression pressure distribution inside of a 25 cm2 PEM electrolysis cell was evaluated, using different materials: Teflon®, Viton®, ethylene propylene diene monomer rubber (EPDM), and nitrile rubber. Sealing material evaluation was performed taking as performance indicators: total compressed area (%) and compression pressure, for different torques applied. Pressure distribution was obtained by using pressure-sensitive films, analyzing the distribution of pressure points from three-dimensional plots (3D), and quantifying intensities of the images obtained. Results showed that pressure points distribution depends on the stiffness and thickness of the gasket materials. For a tightening torque of 3.70 N m, a pressure of 2.23 MPa is obtained with 85% of the membrane area compressed using nitrile rubber-EPDM gaskets. 相似文献
63.
Abstract Accurate numerical calculations are carried out to investigate the validity of the two-state approximation in the case of resonant interactions between electromagnetic radiation and atoms. Short pulses are considered and the presence of the atomic spectrum is modelled by introducing a third, non-resonant, state. We show that the harmonics of the pulse profile may play a significant role in the dynamics of the process and may cause energy non-conserving transitions between the atomic states. 相似文献
64.
The dependence of the fluorescence quenching of electropolymerized poly(aniline-co-m-chloroaniline) with polymer composition has been investigated. Fluorescence emission in polyaniline is quenched when the polymer is oxidized (brought to emeraldine state); the copolymers exhibit decreasing quenching as chloroaniline contents increases. Quenching shows a strong decrease in the presence of 0.1% m-chloroaniline monomers in the feed. The presence of dichloroaniline units in the copolymer was confirmed by XPS measurements and a terpolymerization reaction scheme was developed, obtaining the kinetic parameters. By Monte Carlo simulation the sequence length distributions for different compositions were obtained and compared; it was found that quenching, for low aniline contents, requires aniline sequences of at least three units. The strong decrease in quenching at low m-chloroaniline contents is attributed to a double effect: breaking of conjugation in the emeraldine form by the presence of the chlorinated unit, and a disruption of the close chain packing in the crystalline domains, preventing state delocalization and thus efficient quenching. 相似文献
65.
66.
In November 2012 the Google Scholar Metrics (GSM) journal rankings were updated, making it possible to compare bibliometric indicators in the ten languages indexed—and their stability—with the April 2012 version. The h-index and h-5 median of 1,000 journals were analysed, comparing their averages, maximum and minimum values and the correlation coefficient within rankings. The bibliometric figures grew significantly. In just seven and a half months the h-index of the journals increased by 15 % and the median h-index by 17 %. This growth was observed for all the bibliometric indicators analysed and for practically every journal. However, we found significant differences in growth rates depending on the language in which the journal is published. Moreover, the journal rankings seem to be stable between April and November, reinforcing the credibility of the data held by Google Scholar and the reliability of the GSM journal rankings, despite the uncontrolled growth of Google Scholar. Based on the findings of this study we suggest, firstly, that Google should upgrade its rankings at least semi-annually and, secondly, that the results should be displayed in each ranking proportionally to the number of journals indexed by language. 相似文献
67.
68.
José Ignacio Gimenez-Nadal José Alberto Molina Jorge Velilla 《Papers in Regional Science》2019,98(4):1667-1684
The research on commuting has emerged in recent decades, but the issue of whether the empirical techniques used are appropriate has not been analysed. Thus, results from prior research could be based on non-accurate models, leading to misleading conclusions. We apply an algorithmic approach based on bootstrapping, variable selection, and mean absolute prediction errors, which is designed to avoid overfitting. Using the American Time Use Survey, we find that models with a reduced set of explanatory variables have similar accuracy to standard econometric models. Our results shed light on the importance of determining whether models can be overfitted. 相似文献
69.
70.
Annamaria Perna Amalia Simonetti Vita Acquaviva Rocco Rossano Giulia Grassi Emilio Gambacorta 《International Journal of Food Science & Technology》2020,55(5):2020-2028
The aim of this study was to estimate the hydrolytic degree and antioxidant activity of purified casein characterised by different haplotypes (αs1-, β- and k-casein) after in vitro digestion with two different enzymatic systems: pepsin from porcine gastric mucosa (EP) and a crude enzymatic extract from the edible mushroom Pleurotus eryngii. The used enzymes showed a different mode of casein catalysis with a consequent production of peptides of different antioxidant activity. The CN haplotype significantly influenced peptides production; in fact, the amino acid substitutions caused by genetic polymorphisms at the αs1-, β- and k-CN loci influenced the sites of enzymatic cleavage and therefore the produced peptides. The above is evidenced by the different antioxidant activity found in the hydrolysates depending on the used enzymatic system, the CN haplotype, and the CN haplotype × enzymatic treatment interaction. The findings of this study are a perspective for the production of specific foods that exert a biological effect in addition to the nutritional one. 相似文献