一株丝状真菌发酵法产红色素培养基的研究

研究了一株丝状真菌Miug-2．7发酵法生产红色素的液体培养基最佳组分，以及液体发酵培养基最佳配方．结果表明，本试验范围内最佳质量配比是淀粉3％，蛋白胨1．0％，诱导物1％，起始pH7．5．     

先进制造技术与我国加入WTO后制造业前瞻

介绍了先进制造技术的发展历程及其系统体系，重点阐述了现代制造系统工程设计技术的重要地位，并对我国加入世贸组织后制造业前景作了简要展望．     

基于快速原型制造技术的产品开发

介绍了适应市场的快速响应设计技术的内容，关键技术和具体实施策略，快速响应设计在企业竞争中的作用．同时说明了快速原型制造技术在快速制造中的作用．     

TiO_2系薄膜型氧敏材料的研究与发展综述

介绍了TiO2薄膜型氧敏材料的特性、氧敏机理及发展优势。就TiO2薄膜型氧敏材料的制备新方法和几种新型TiO2系薄膜以及相应的氧敏性能等重要方面进行了探讨,并对今后的研究与发展方向提出几点建议。     

Identifying critical autonomous systems in the Internet

The Internet not only facilitates our daily activities, such as communication, entertainment and shopping but also serves as the enabling technology for many critical services, including finance, manufacturing, healthcare and transportation. On the other hand, a wide spectrum of attacks targets its communication infrastructure to disable or disrupt the network connectivity and traffic flow until recovery processes take place. Attacking all autonomous systems (ASes) in the Internet is typically beyond the capability of an adversary. Therefore, targeting a small number of ASes which results in the highest impact is the best strategy for attackers. Similarly, it is important for network practitioners to identify, fortify and secure those critical ASes to mitigate the impact of the attacks. In this study we introduce an intuitive and effective measure, IP address spatial path stress centrality, to assess and identify the critical ASes in the Internet. We compare IP address spatial path stress centrality to the three well-known and widely used centrality measures, namely customer-cone size, node degree and betweenness. We demonstrate that the proposed measure incorporates business relations and IP address spaces to achieve a better measure for identifying the critical ASes in the Internet.     

DNA-directed immobilization of horseradish peroxidase onto porous SiO2optical transducers

ABSTRACT: Multifunctional porous Si nanostructure is designed to optically monitor enzymatic activity of Horseradish Peroxidase. First, an oxidized PSi optical nanostructure, a Fabry-Perot thin film, is synthesized and is used as the optical transducer element. Immobilization of the enzyme onto the nanostructure is performed through DNA-Directed Immobilization. Preliminary studies demonstrate high enzymatic activity levels of the immobilized Horseradish Peroxidase, while maintaining its specificity. The catalytic activity of the enzymes immobilized within the porous nanostructure is monitored in real time by reflective interferometric Fourier transform spectroscopy. We show that we can easily regenerate the surface for consecutive biosensing analysis by mild de-hybridization conditions.     

Chemical and microbiological status and volatile profiles of mouldy Civil cheese,a Turkish mould‐ripened variety

The objective of this study is to characterise the gross chemical and microbiological status and identify the volatile compounds of mouldy Civil cheeses. A total of forty‐one samples were surveyed, and gross compositional status of the cheeses was (as mean values): 6.5 for pH, 6.2% for fat‐in‐dry matter, 51.8% for moisture and 15.3% for water‐soluble nitrogen (as% of total nitrogen). Chemical composition of the cheese samples varied widely. Mouldy Civil cheese has similar pH values and moisture contents when compared with blue‐type cheeses, but it has distinct feature for fat contents. The microbiological counts of the samples were found to be high and some samples contained coliform bacteria. A total of 95 volatiles, including esters (28), acids (6), ketones (12), aldehydes (3), alcohols (15), terpenes (10), sulphur compounds (3) and miscellaneous (18), were identified in the volatile fractions of the cheeses, and principal volatile groups were esters, alcohols and ketones.     

Examination of the ECG signal classification technique DEA-ELM using deep convolutional neural network features

Multimedia Tools and Applications - The accurate separation of ECG signals has become crucial to identify heart diseases. Machine learning methods are widely used to separate ECG signals. The aim...     

Self-sufficient Cytochrome P450s and their potential applications in biotechnology

Cytochrome P450s(CYPs) are ubiquitously found in all kingdoms of life, playing important role in various biosynthetic pathways as well as degradative pathways; accordingly find applications in a vast variety of areas from organic synthesis and drug metabolite production to modification of biomaterials and bioremediation.Significantly, CYPs catalyze chemically challenging C—H and C—C activation reactions using a reactive high-valent iron-oxo intermediate generated upon dioxygen activation at their heme center,while the other oxygen atom is reduced to the level of water by electrons provided through a reductase partner protein.Self-sufficient CYPs, encoding their heme domain and reductase protein in a single polypeptide, facilitate increased catalytic efficiency and render a less complicated system to work with.The self-sufficient CYP enzyme from CYP102A family(CYP102A1, BM3) is among the earliest and most-investigated model enzymes for mechanistic and structural studies as well as for biotechnological applications.An increasing number of self-sufficient CYPs from the same CYP102 family and from other families have also been reported in last decade.In this review, we introduce chemistry and biology of CYPs, followed by an overview of the characteristics of self-sufficient CYPs and representative reactions.Enzyme engineering efforts leading to novel self-sufficient CYP variants that can catalyze synthetically useful natural and non-natural(nature-mimicking) reactions are highlighted.Lastly, the strategy and efforts that aim to circumvent the challenges for improved thermostability, regio-and enantioselectivity,and total turnover number; associated with practical use of self-sufficient CYPs are reviewed.     

Preparation of Porous Ca10(PO4)6(OH)2 and β-Ca3(PO4)2 Bioceramics

Submicrometer-sized, pure calcium hydroxyapatite (HA, (Ca₁₀(PO₄)₆(OH)₂)) and β-tricalcium phosphate (β-TCP, Ca₃(PO₄)₂) bioceramic powders, that have been synthesized via chemical precipitation techniques, were used in the preparation of aqueous slurries that contained methyl cellulose to manufacture porous (70%–95% porosity) HA or β-TCP ceramics. The pore sizes in HA bioceramics of this study were 200–400 μm, whereas those of β-TCP bioceramics were 100–300 μm. The pore morphology and total porosity of the HA and β-TCP samples were investigated via scanning electron microscopy, water absorption, and computerized tomography.