Vivien M. Kiyek  Yorick A. Birkhlzer  Yury Smirnov  Martin Ledinsky  Zdenek Remes  Jamo Momand  Bart J. Kooi  Gertjan Koster  Guus Rijnders  Monica Morales‐Masis 《Advanced Materials Interfaces》2020,7(11)

The presence of a nonoptically active polymorph (yellow‐phase) competing with the optically active polymorph (black γ‐phase) at room temperature in cesium tin iodide (CsSnI₃) and the susceptibility of Sn to oxidation represent two of the biggest obstacles for the exploitation of CsSnI₃ in optoelectronic devices. Here room‐temperature single‐source in vacuum deposition of smooth black γ −CsSnI₃ thin films is reported. This is done by fabricating a solid target by completely solvent‐free mixing of CsI and SnI₂ powders and isostatic pressing. By controlled laser ablation of the solid target on an arbitrary substrate at room temperature, the formation of CsSnI₃ thin films with optimal optical properties is demonstrated. The films present a bandgap of 1.32 eV, a sharp absorption edge, and near‐infrared photoluminescence emission. These properties and X‐ray diffraction of the thin films confirm the formation of the orthorhombic (B‐γ ) perovskite phase. The thermal stability of the phase is ensured by applying in situ an Al₂O₃ capping layer. This work demonstrates the potential of pulsed laser deposition as a volatility‐insensitive single‐source growth technique of halide perovskites and represents a critical step forward in the development and future scalability of inorganic lead‐free halide perovskites.  相似文献   

    

Matías N. Hadad  Marcos A. Funes  Patricio G. Donato  Enrique García  Jesús Ureña  Daniel Carrica 《International Journal of Circuit Theory and Applications》2016,44(3):549-561

New coding schemes are increasingly being developed to adapt multisensor and communication systems to the requirements of the current technological environment. In some cases, the practical implementation of these schemes involves a great amount of signal processing operations. In order to achieve and make suitable their use in different platforms, efficient algorithms are required to process coding. In the last years, a new kind of complementary sequences, known as Generalized Pairwise Complementary sequences, has been proposed. These sequences provide high noise immunity when they are applied to multiuser systems, as well as a low cross‐correlation, which is convenient in those applications. The aim of this paper is to propose optimized generation and correlation architectures for Generalized Pairwise Complementary sequences that would allow to process a complete set using a reduced amount of operations. An analytical demonstration is provided, with a final comparison to evaluate the reduction with regard to other algorithms. Copyright © 2015 John Wiley & Sons, Ltd.  相似文献   

    

Chao Yang  Yuanpeng Yao  Yong Huan  Zhiqiang Fu  Fei Chen  Enrique J. Lavernia 《Advanced Engineering Materials》2016,18(11):1936-1943

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Sylwia Nowakowska  Aneliia Wäckerlin  Ignacio Piquero‐Zulaica  Jan Nowakowski  Shigeki Kawai  Christian Wäckerlin  Manfred Matena  Thomas Nijs  Shadi Fatayer  Olha Popova  Aisha Ahsan  S. Fatemeh Mousavi  Toni Ivas  Ernst Meyer  Meike Stöhr  J. Enrique Ortega  Jonas Björk  Lutz H. Gade  Jorge Lobo‐Checa  Thomas A. Jung 《Small (Weinheim an der Bergstrasse, Germany)》2016,12(28):3757-3763

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Sara Garcinuo  Francisco Javier Gil-Etayo  Esther Mancebo  Marta Lpez-Nevado  Antonio Lalueza  Raquel Díaz-Simn  Daniel Enrique Pleguezuelo  Manuel Serrano  Oscar Cabrera-Marante  Luis M. Allende  Estela Paz-Artal  Antonio Serrano 《International journal of molecular sciences》2022,23(12)

NK degranulation plays an important role in the cytotoxic activity of innate immunity in the clearance of intracellular infections and is an important factor in the outcome of the disease. This work has studied NK degranulation and innate immunological profiles and functionalities in COVID-19 patients and its association with the severity of the disease. A prospective observational study with 99 COVID-19 patients was conducted. Patients were grouped according to hospital requirements and severity. Innate immune cell subpopulations and functionalities were analyzed. The profile and functionality of innate immune cells differ between healthy controls and severe patients; CD56dim NK cells increased and MAIT cells and NK degranulation rates decreased in the COVID-19 subjects. Higher degranulation rates were observed in the non-severe patients and in the healthy controls compared to the severe patients. Benign forms of the disease had a higher granzymeA/granzymeB ratio than complex forms. In a multivariate analysis, the degranulation capacity resulted in a protective factor against severe forms of the disease (OR: 0.86), whereas the permanent expression of NKG2D in NKT cells was an independent risk factor (OR: 3.81; AUC: 0.84). In conclusion, a prompt and efficient degranulation functionality in the early stages of infection could be used as a tool to identify patients who will have a better evolution.  相似文献   

    

Carlota Arias-Hidalgo  Pablo Juanes-Velasco  Alicia Landeira-Viuela  Marina L. García-Vaquero  Enrique Montalvillo  Rafael Gngora  ngela-Patricia Hernndez  Manuel Fuentes 《International journal of molecular sciences》2022,23(12)

In single-cell analysis, biological variability can be attributed to individual cells, their specific state, and the ability to respond to external stimuli, which are determined by protein abundance and their relative alterations. Mass spectrometry (MS)-based proteomics (e.g., SCoPE-MS and SCoPE2) can be used as a non-targeted method to detect molecules across hundreds of individual cells. To achieve high-throughput investigation, novel approaches in Single-Cell Proteomics (SCP) are needed to identify and quantify proteins as accurately as possible. Controlling sample preparation prior to LC-MS analysis is critical, as it influences sensitivity, robustness, and reproducibility. Several nanotechnological approaches have been developed for the removal of cellular debris, salts, and detergents, and to facilitate systematic sample processing at the nano- and microfluidic scale. In addition, nanotechnology has enabled high-throughput proteomics analysis, which have required the improvement of software tools, such as DART-ID or DO-MS, which are also fundamental for addressing key biological questions. Single-cell proteomics has many applications in nanomedicine and biomedical research, including advanced cancer immunotherapies or biomarker characterization, among others; and novel methods allow the quantification of more than a thousand proteins while analyzing hundreds of single cells.  相似文献   

    

María Illescas  María E. Morn-Diez  ngel Emilio Martínez de Alba  Rosa Hermosa  Enrique Monte 《International journal of molecular sciences》2022,23(12)

Eight Trichoderma strains were evaluated for their potential to protect wheat seedlings against severe (no irrigation within two weeks) water stress (WS). Considering the plant fresh weight and phenotype, T. asperellum T140, which displays 1-aminocyclopropane-1-carboxylic acid deaminase activity and which is able to produce several phytohormones, was selected. The molecular and biochemical results obtained from 4-week-old wheat seedlings linked T140 application with a downregulation in the WS-response genes, a decrease in antioxidant activities, and a drop in the proline content, as well as low levels of hydrogen peroxide and malondialdehyde in response to severe WS. All of these responses are indicative of T140-primed seedlings having a higher tolerance to drought than those that are left untreated. A greenhouse assay performed under high nitrogen fertilization served to explore the long-term effects of T140 on wheat plants subjected to moderate (halved irrigation) WS. Even though all of the plants showed acclimation to moderate WS regardless of T140 application, there was a positive effect exerted by T. asperellum on the level of tolerance of the wheat plants to this stress. Strain T140 modulated the expression of a plant ABA-dependent WS marker and produced increased plant superoxide dismutase activity, which would explain the positive effect of Trichoderma on increasing crop yields under moderate WS conditions. The results demonstrate the effectiveness of T. asperellum T140 as a biostimulant for wheat plants under WS conditions, making them more tolerant to drought.  相似文献   

    

María ngeles Peinado  David Ovelleiro  María Luisa del Moral  Raquel Hernndez  Esther Martínez-Lara  Eva Siles  Jos Rafael Pedrajas  María Luisa García-Martín  Carlos Caro  Sebastin Peralta  María Encarnacin Morales  María Adolfina Ruiz  Santos Blanco 《International journal of molecular sciences》2022,23(1)

Exogenous neuroprotective protein neuroglobin (Ngb) cannot cross the blood–brain barrier. To overcome this difficulty, we synthesized hyaluronate nanoparticles (NPs), able to deliver Ngb into the brain in an animal model of stroke (MCAO). These NPs effectively reached neurons, and were microscopically identified after 24 h of reperfusion. Compared to MCAO non-treated animals, those treated with Ngb-NPs showed survival rates up to 50% higher, and better neurological scores. Tissue damage improved with the treatment, but no changes in the infarct volume or in the oxidative/nitrosative values were detected. A proteomics approach (p-value < 0.02; fold change = 0.05) in the infarcted areas showed a total of 219 proteins that significantly changed their expression after stroke and treatment with Ngb-NPs. Of special interest, are proteins such as FBXO7 and NTRK2, which were downexpressed in stroke, but overexpressed after treatment with Ngb-NPs; and ATX2L, which was overexpressed only under the effect of Ngb. Interestingly, the proteins affected by the treatment with Ngb were involved in mitochondrial function and cell death, endocytosis, protein metabolism, cytoskeletal remodeling, or synaptic function, and in regenerative processes, such as dendritogenesis, neuritogenesis, or sinaptogenesis. Consequently, our pharmaceutical preparation may open new therapeutic scopes for stroke and possibly for other neurodegenerative pathologies.  相似文献   

    

Camila Reyes  Estefanía Nova-Lamperti  Daniel Duran-Sandoval  Daniela Rojas  Jorge Gajardo  Enrique Guzman-Gutierrez  Camila Bustos-Ruiz  Valeska Ormazbal  Felipe A. Zúiga  Carlos Escudero  Claudio Aguayo 《International journal of molecular sciences》2022,23(13)

Oxidized low-density lipoprotein (ox-LDL) is the most harmful form of cholesterol associated with vascular atherosclerosis and hepatic injury, mainly due to inflammatory cell infiltration and subsequent severe tissue injury. Lox-1 is the central ox-LDL receptor expressed in endothelial and immune cells, its activation regulating inflammatory cytokines and chemotactic factor secretion. Recently, a Lox-1 truncated protein isoform lacking the ox-LDL binding domain named LOXIN has been described. We have previously shown that LOXIN overexpression blocked Lox-1-mediated ox-LDL internalization in human endothelial progenitor cells in vitro. However, the functional role of LOXIN in targeting inflammation or tissue injury in vivo remains unknown. In this study, we investigate whether LOXIN modulated the expression of Lox-1 and reduced the inflammatory response in a high-fat-diet mice model. Results indicate that human LOXIN blocks Lox-1 mediated uptake of ox-LDL in H4-II-E-C3 cells. Furthermore, in vivo experiments showed that overexpression of LOXIN reduced both fatty streak lesions in the aorta and inflammation and fibrosis in the liver. These findings were associated with the down-regulation of Lox-1 in endothelial cells. Then, LOXIN prevents hepatic and aortic tissue damage in vivo associated with reduced Lox-1 expression in endothelial cells. We encourage future research to understand better the underlying molecular mechanisms and potential therapeutic use of LOXIN.  相似文献   

    

Yi Zhao  Eliud Morales Dvila  Xue Li  Beiyu Tang  Ariana I. Rabinowitsch  Jose Manuel Perez-Aguilar  Carl P. Blobel 《International journal of molecular sciences》2022,23(21)

The metalloprotease ADAM17 is a key regulator of the TNFα, IL-6R and EGFR signaling pathways. The maturation and function of ADAM17 is controlled by the seven-membrane-spanning proteins iRhoms1 and 2. The functional properties of the ADAM17/iRhom1 and ADAM17/iRhom2 complexes differ, in that stimulated shedding of most ADAM17 substrates tested to date can be supported by iRhom2, whereas iRhom1 can only support stimulated shedding of very few ADAM17 substrates, such as TGFα. The first transmembrane domain (TMD1) of iRhom2 and the sole TMD of ADAM17 are important for the stimulated shedding of ADAM17 substrates by iRhom2. However, little is currently known about how the iRhoms interact with different substrates to control their stimulated shedding by ADAM17. To provide new insights into this topic, we tested how various chimeras between iRhom1 and iRhom2 affect the stimulated processing of the EGFR-ligands TGFα (iRhom1- or 2-dependent) and EREG (iRhom2-selective) by ADAM17. This uncovered an important role for the TMD7 of the iRhoms in determining their substrate selectivity. Computational methods utilized to characterize the iRhom1/2/substrate interactions suggest that the substrate selectivity is determined, at least in part, by a distinct accessibility of the substrate cleavage site to stimulated ADAM17. These studies not only provide new insights into why the substrate selectivity of stimulated iRhom2/ADAM17 differs from that of iRhom1/ADAM17, but also suggest new approaches for targeting the release of specific ADAM17 substrates.  相似文献